Human Klotho  beta Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Klotho beta

Klotho beta (b-Klotho or KLB) is a 125-135 kDa member of the KL family, Glycosyl Hydrolase 1 superfamily of proteins.  It plays key role in the biology of the endocrine FGFs (FGF-15/19; 21; 23).  KLB appears to serve as a signaling cofactor for select FGFRs, including FGFR-1c, -2c, -3c and FGFR4.  The cofactor is known to constitutively interact with FGFRs, promote FGF binding to their appropriate FGFRs, and to bind directly to FGF-19 and FGF-21.  Notably, FGF-23 appears to utilize a-Klotho/KL for its FGFR signaling cofactor, and FGF-1, while not dependent upon KLB for signaling, can utilize KBL to potentiate certain activities.  KLB is expressed on select cells, including adipocytes and hepatocytes.  Human KLB is a 1044 amino acids (aa) type III transmembrane (TM) protein (i.e.-a type I TM protein lacking a signal sequence).  It contains a 996 aa extracellular region (aa 1-996) and a 27 aa cytoplasmic domain (aa 1018-1044).  The extracellular region contains two glycosyl hydrolase domains (aa 77-508 and 517-967) plus eleven potential N-linked glycosylation sites. Neither hydrolase domain is active as they lack a key Glu residue needed for activity.  Over aa 53-997, human KLB shares 80% aa sequence identity with mouse KLB.  And relative to KL/a-Klotho, human KLB and KL share just 44% aa sequence identity over the entire length of the molecules.     

Product Datasheets

Related Research Areas

• FGF Family
• Glycobiology-related Enzymes
• Lipid/Cholesterol Metabolism
• Lysosomal Enzymes
• Search Bioprocessing Enzymes
• Search Glycobiology-related Enzymes