Human Klotho Antibody Summary
Glu34-Ser981
Accession # Q9UEF7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Cell Proliferation Induced by Klotho and Neutralization by Human Klotho Antibody. Recombinant Human Klotho (Catalog # 5334-KL) induces proliferation in the BaF3 mouse pro-B cell line transfected with human FGF RIIIc in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Klotho (0.3 µg/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Human Klotho Monoclonal Antibody (Catalog # MAB5334). The ND50 is typically 0.15-0.6 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Klotho
Klotho, also called Klotho-alpha, is the founding member of the Klotho family within the glycosidase-1 superfamily (1, 2). Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid (1). The 1012 amino acid (aa) type I transmembrane protein contains a 32 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and an 11 aa intracellular domain. Within the ECD, human Klotho shares 87%, 90%, 90% and 86% aa identity with mouse, rat, bovine and equine Klotho, respectively. Although a truncated 549 aa isoform predicts a soluble 70 kDa form, the form found in plasma and cerebrospinal fluid is a 130 kDa form produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho (3, 4). A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes (4). Klotho is named for the Greek goddess who spins the thread of life. The phenotype of Klotho‑deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema and premature death (2). Conversely, excess Klotho extends lifespan (5). Klotho acts as a cofactor for interaction of FGF23 with FGF R1 (6). This interaction negatively regulates 1 alpha -hydroxylase, the rate‑limiting enzyme in the synthesis of 1,25(OH)2D3 (vitamin D)(7). Klotho‑deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D (2, 7). Both Klotho and Klotho-beta are cofactors for FGF19 binding (8). Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium (9). Klotho has been reported to downregulate insulin or IGF-1 signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone (10-12).
- Nabeshima, Y. (2006) Sci. Aging Knowl. Environ. 8:pe11.
- Kuro-o, M. et al. (1997) Nature 390:45.
- Shiraki-Iida, T. et al. (1998) FEBS Lett. 424:6.
- Imura, A. et al. (2004) FEBS Lett. 565:143.
- Kurosu, H. et al. (2005) Science 309:1829.
- Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
- Tsujikawa, H. et al. (2003) Mol. Endocrinol. 17:2393.
- Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
- Chang, Q. et al. (2005) Science 310:490.
- Yamamoto, M. et al. (2005) J. Biol. Chem. 280:38029.
- Liu, H. et al. (2007) Science 317:803.
- Imura, A. et al. (2007) Science 316:1615.
Product Datasheets
Citations for Human Klotho Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
Molecular Interaction of Soluble Klotho with FGF23 in the Pathobiology of Aortic Valve Lesions Induced by Chronic Kidney Disease
Authors: The, E;Zhai, Y;Yao, Q;Ao, L;Fullerton, DA;Meng, X;
International journal of biological sciences
Species: Mouse, Transgenic Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Protective effects of klotho on palmitate-induced podocyte injury in diabetic nephropathy
Authors: J Suk Kang, SS Son, JH Lee, SW Lee, AR Jeong, ES Lee, SK Cha, CH Chung, EY Lee
PLoS ONE, 2021-04-23;16(4):e0250666.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease
Authors: X Zhong, S Jagarlapud, Y Weng, M Ly, JC Rouse, K McClure, T Ishino, Y Zhang, E Sousa, J Cohen, B Tzvetkova, K Cote, JJ Scarcelli, K Johnson, J Palandra, JR Apgar, S Yaddanapud, RG Villalobos, AC Opsahl, K Lam, Q Yao, W Duan, A Sievers, J Zhou, D Ferguson, A D'Antona, R Zollner, HL Zhu, R Kriz, L Lin, V Clerin
J. Biol. Chem., 2020-01-31;0(0):.
Species: Rat
Sample Types: Serum
Applications: Immunoprecipitation
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Klotho Antibody
There are currently no reviews for this product. Be the first to review Human Klotho Antibody and earn rewards!
Have you used Human Klotho Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
