Human KGF/FGF-7 Biotinylated Antibody Summary
Cys32-Thr194
Accession # Q6FGV5
Applications
Human KGF/FGF-7 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: KGF/FGF-7
KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1 - 4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.
- Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
- Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
- Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
- Mason, I.J. et al. (1994) Mech. Dev. 45:15.
- Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
- Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
- Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
- Qiao, J. et al. (1999) Development 126:547.
- Guo, L. et al. (1996) Genes Dev. 10:165.
- de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
- Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
- Belleudi, F. et al. (2007) Traffic 8:1854.
Product Datasheets
Citation for Human KGF/FGF-7 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Induction of keratinocyte growth factor 1 Expression by lipopolysaccharide is regulated by CD-14 and toll-like receptors 2 and 4.
Authors: Putnins EE, Sanaie AR, Wu Q
Infect. Immun., 2002-12-01;70(12):6541-8.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human KGF/FGF-7 Biotinylated Antibody
Average Rating: 4 (Based on 1 Review)
Have you used Human KGF/FGF-7 Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
