Home / Isocitrate Dehydrogenase 1/IDH1 / Human Isocitrate Dehydrogenase 1/IDH1 Antibody

Human Isocitrate Dehydrogenase 1/IDH1 Antibody

Catalog #: MAB7049
6 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB7049
MAB7049-SP
Detection of Human, Mouse, and Rat Isocitrate Dehydrogenase 1/IDH1 by Western Blot.
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Citations (6)
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Human Isocitrate Dehydrogenase 1/IDH1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Isocitrate Dehydrogenase 1/IDH1 in ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 843219
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Isocitrate Dehydrogenase 1/IDH1
Ser2-Leu414
Accession # O75874
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
10 µg/mL
HepG2 human hepatocellular carcinoma cell line
Immunohistochemistry
8-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below
Knockout Validated
Isocitrate Dehydrogenase 1/IDH1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Isocitrate Dehydrogenase 1/IDH1 knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Isocitrate Dehydrogenase 1/IDH1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Isocitrate Dehydrogenase 1/IDH1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Isocitrate Dehydrogenase 1/IDH1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Isocitrate Dehydrogenase 1/IDH1 antibody in SK-BR-3 Human Cell Line by Immunocytochemistry (ICC). View Larger

Isocitrate Dehydrogenase 1/IDH1 in SK‑BR‑3 Human Cell Line. Isocitrate Dehydrogenase 1/IDH1 was detected in immersion fixed SK-BR-3 human breast cancer cell line using Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Isocitrate Dehydrogenase 1/IDH1 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Isocitrate Dehydrogenase 1/IDH1 in Human Brain. Isocitrate Dehydrogenase 1/IDH1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to astrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Isocitrate Dehydrogenase 1/IDH1 antibody in Rat Brain by Immunohistochemistry (IHC-Fr). View Larger

Isocitrate Dehydrogenase 1/IDH1 in Rat Brain. Isocitrate Dehydrogenase 1/IDH1 was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to glial cell cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Knockout Validated Western Blot Shows Human Isocitrate Dehydrogenase 1/IDH1 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Isocitrate Dehydrogenase 1/IDH1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Isocitrate Dehydrogenase 1/IDH1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Isocitrate Dehydrogenase 1/IDH1 at approximately 46 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human Isocitrate Dehydrogenase 1/IDH1 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Isocitrate Dehydrogenase 1/IDH1 at approximately 51 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Isocitrate Dehydrogenase 1/IDH1 Monoclonal Antibody (Catalog # MAB7049). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Isocitrate Dehydrogenase 1/IDH1

Isocitrate Dehydrogenase 1 (IDH1) catalyzes the oxidative decarboxylation of isocitrate to alpha ‑ketoglutarate. There are two subclasses in the IDH family, one of them utilizing NADP+ as the electron acceptor and the other using NAD+ (1). The protein encoded by this gene is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. In peroxisomes, IDH1 generates the NADPH required for intraperoxisomal reduction reactions. Mutations of Arg132 of human IDH1 result in a reduced ability of the enzyme to convert isocitrate to alpha ‑ketoglutarate, but the enzyme acquires the ability to generate 2-hydroxyglutarate (2HG) from  alpha ‑ketoglutarate (2). Elevated levels of the metabolite 2HG are associated with a high risk of malignant brain tumors. Arg132 mutations of IDH1 are common in high‑grade gliomas, but not in other types of tumors (3).

References
  1. Nekrutenko, A. et al. (1998) Mol. Biol. Evol. 15:1674.
  2. Dang, L. et al. (2009) Nature 462:739.
  3. Bleeker, F.E. et al. (2009) Hum. Mutat. 30:7.
Entrez Gene IDs
3417 (Human); 15926 (Mouse); 24479 (Rat)
Alternate Names
Cytosolic NADP-isocitrate dehydrogenase; EC 1.1.1.42; IDCD; IDH; IDH1; IDP; IDPC; isocitrate dehydrogenase [NADP] cytoplasmic; isocitrate dehydrogenase 1 (NADP+), soluble; Isocitrate Dehydrogenase 1; NADP(+)-specific ICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; Oxalosuccinate decarboxylase; PICD

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Citations for Human Isocitrate Dehydrogenase 1/IDH1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Lysine acetylation restricts mutant IDH2 activity to optimize transformation in AML cells
    Authors: Chen D, Xia S, Zhang R Et Al.
    Molecular cell
  2. TNFalpha-induced IDH1 hyperacetylation reprograms redox homeostasis and promotes the chemotherapeutic sensitivity
    Authors: H Yang, X Zhao, J Liu, M Jin, X Liu, J Yan, X Yao, X Mao, N Li, B Liang, W Xie, K Zhang, J Zhao, L Liu, G Huang
    Oncogene, 2022-11-10;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  3. Cellular signals converge at the NOX2-SHP-2 axis to induce reductive carboxylation in cancer cells
    Authors: Rukang Zhang, Dong Chen, Hao Fan, Rong Wu, Jiayi Tu, Freya Q. Zhang et al.
    Cell Chemical Biology
  4. Exosomal IDH1 increases the resistance of colorectal cancer cells to 5-Fluorouracil
    Authors: H Yang, S Xie, B Liang, Q Tang, H Liu, D Wang, G Huang
    Journal of Cancer, 2021-06-11;12(16):4862-4872.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Metabolic Diversity in Human Non-Small Cell Lung Cancer Cells
    Authors: Pei-Hsuan Chen, Ling Cai, Kenneth Huffman, Chendong Yang, Jiyeon Kim, Brandon Faubert et al.
    Molecular Cell
  6. Mutant and wild-type isocitrate dehydrogenase 1 share enhancing mechanisms involving distinct tyrosine kinase cascades in cancer
    Authors: D Chen, S Xia, M Wang, R Lin, Y Li, H Mao, M Aguiar, CA Famulare, AH Shih, CW Brennan, X Gao, Y Pan, S Liu, J Fan, L Jin, L Song, A Zhou, J Mukherjee, RO Pieper, A Mishra, J Peng, M Arellano, WG Blum, S Lonial, TJ Boggon, RL Levine, J Chen
    Cancer Discov, 2019-03-12;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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