Human IRF3 APC-conjugated Antibody

Catalog #: IC4019A Datasheet

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IC4019A

Detection of IRF3 in Daudi Human Cell Line by Flow Cytometry.

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Human IRF3 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IRF3 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 482205

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IRF3
aa 206-427
Accession # Q14653

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of IRF3 antibody in Daudi Human Cell Line antibody by Flow Cytometry.

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Detection of IRF3 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IRF3 APC-conjugated Monoclonal Antibody (Catalog # IC4019A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IRF3

IRF3 (interferon response factor 3) is a 60 kDa member of the IRF family of proteins. Human IRF3 contains one DNA binding domain (aa 7‑107), a nuclear export signal (aa 139‑149) and multiple phosphorylation sites (aa 395‑407). Viral infection stimulates IRF3 phosphorylation, nuclear translocation and stimulation of IFN production. Alternate splice forms may exist. One will show a deletion of aa 201‑327, a second will show the same deletion plus an alternate start site at Met147, and a third will show a 125 aa substitution for the C-terminal 100 aa (aa 328‑427). Over aa 206‑427, human IRF3 is 76% and 83% aa identical to mouse and pig IRF3, respectively.

Long Name

Interferon Regulatory Factor 3

Entrez Gene IDs

3661 (Human); 54131 (Mouse)

Alternate Names

interferon regulatory factor 3; IRF3; IRF-3