Human IRF3 Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: IRF3

Interferon response factor 3 (IRF3) is a 50 kDa, 427 amino acid, member of the IRF family of proteins. Human IRF3 is a transcription factor that binds the interferon-sensitive response element (ISRE) and is activated by Toll-like receptors, TLR3 and TLR4. Viral entry or engagement of TLR3 or TLR4 stimulates IRF3 phosphorylation, nuclear translocation and stimulation of IFN production. There are potential alternate splice forms affecting IRF3 structure, one including a deletion of aa 201-327, a second with the same deletion and an alternate Met(147) start site, and a third with a 125 aa substitution of the C-terminal 100 aa. Over the region used as immunogen, aa 206-427, human IRF3 is 76% and 83% identical to the corresponding mouse and porcine proteins, respectively.

Product Datasheets

Related Research Areas

• Interferon-Regulatory Factor Transcription Factors
• RIG-I-like Receptors
• Toll-Like Receptors
• Transcription Factors in the Akt Pathway
• Transcription Regulators in VSMC