Human IL-8/CXCL8 PE-conjugated Antibody

Catalog #: IC208P
4 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
IC208P

Detection of CXCL8/IL‑8 in Human Blood Monocytes by Flow Cytometry.

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All IL-8/CXCL8 Antibodies

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Citations (4)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-8/CXCL8 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXCL8/IL‑8 in ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant porcine CXCL8/IL‑8 and no cross-reactivity with rrCXCL3/CINC-2 alpha.

Source

Monoclonal Mouse IgG₁ Clone # 6217

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CXCL8/IL‑8
Ser28-Ser99
Accession # P10145

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of CXCL8/IL-8 antibody in Human Blood Monocytes antibody by Flow Cytometry.

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Detection of CXCL8/IL‑8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CXCL8/IL-8 PE-conjugated Monoclonal Antibody (Catalog # IC208P) and Anti-Human CD14 Fluorescein-conjugated Antibody (panel A). Inhibition of IC208P staining by the addition of excess Recombinant Human IL-8 (Catalog # 208-IL) is shown in panel B. Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-8/CXCL8

CXCL8, also known as IL-8, is a CXC chemokine that is a potent chemoattractant for neutrophils and has a wide range of other pro-inflammatory effects.

Long Name

Interleukin 8

Entrez Gene IDs

3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)

Alternate Names

3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human IL-8/CXCL8 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Downregulation of Pro-Inflammatory and Pro-Angiogenic Pathways in Prostate Cancer Cells by a Polyphenol-Rich Extract from Olive Mill Wastewater
Authors: D Baci, M Gallazzi, C Cascini, M Tramacere, D De Stefano, A Bruno, DM Noonan, A Albini
Int J Mol Sci, 2019-01-14;20(2):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
TGFb1 suppresses the activation of distinct dNK subpopulations in preeclampsia
Authors: J Zhang, CE Dunk, O Shynlova, I Caniggia, SJ Lye
EBioMedicine, 2018-12-19;39(0):531-539.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Gene and protein characteristics reflect functional diversity of CD56dim and CD56bright NK cells.
Authors: Wendt K, Wilk E, Buyny S, Buer J, Schmidt RE, Jacobs R
J. Leukoc. Biol., 2006-09-11;80(6):1529-41.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Different domains of Pseudomonas aeruginosa exoenzyme S activate distinct TLRs.
Authors: Epelman S, Stack D, Bell C, Wong E, Neely GG, Krutzik S, Miyake K, Kubes P, Zbytnuik LD, Ma LL, Xie X, Woods DE, Mody CH
J. Immunol., 2004-08-01;173(3):2031-40.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry