Human IL-8/CXCL8 Fluorescein-conjugated Antibody Summary
Ser28-Ser99
Accession # P10145
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CXCL8/IL‑8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CXCL8/IL-8 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC208F) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P), as shown in panel A. Inhibition of IC208F staining by the addition of excess Recombinant Human IL-8 (Catalog # 208-IL) is shown in panel B. Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Background: IL-8/CXCL8
CXCL8/IL-8 is an 8-9 kDa member of the CXCL subfamily of chemokines. It was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It has also referred to as Neutrophil Activating Protein (NAP), Monocyte-derived Neutrophil Chemotactic Factor (MDNCF) and granulocyte chemotactic protein (GCP). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and mammary plus alveolar epithelium can produce CXCL8 in response to a wide variety of stimuli, such as IL-1b, TNF-a, LPS, MRP-8/14 and viruses. When secreted, it circulates as both a monomer and dimer, with the dimeric forms being a homodimer and heterodimer with CXCL4/PF4. CXCL8 binds to CXCR-1 and -2, with the CXCL8 monomer favoring CXCR-1, and the CXCL8 homodimer favoring CXCR-2 and glycosaminoglycans. CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. It also induces expression of the cell adhesion molecules CD11/CD18, and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes, and has also shown to be an autocrine growth factor for melanoma cells. CXCL8 is also reported to be angiogenic both in vivo and in vitro. There is no direct structural rodent counterpart to human IL-8.
Product Datasheets
Citations for Human IL-8/CXCL8 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Expression of the T cell receptor alpha/beta on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
Authors: Thiel A, Kesselring R, Pries R, Puzik A, Wittkopf N, Wollenberg B
PLoS ONE, 2011-01-11;6(1):e15997.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
J. Clin. Invest., 2010-02-08;120(3):907-23.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Increased expression of inflammation-related co-stimulatory molecules by HUVECs from newborns with a strong family history of myocardial infarction stimulated with TNF-alpha and oxLDL.
Authors: Mendez-Cruz AR, Paez A, Jimenez-Flores R, Reyes-Reali J, Varela E, Cerbulo-Vazquez A, Rodriguez E, Lopez-Marure R, Masso FA, Flores-Romo L, Montano LF
Immunol. Lett., 2007-07-23;111(2):116-23.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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