Human IL-8/CXCL8 Antibody

Catalog #: AF-208-NA
23 Citations 5 Reviews Datasheet

Catalog #	Availability	Size / Price	Qty
AF-208-NA
AF-208-SP

Detection of Human IL-8/CXCL8 by Western Blot.

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All IL-8/CXCL8 Antibodies

Product Details

Citations (23)

FAQs

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Reviews (5)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-8/CXCL8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-8/CXCL8 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IL-8/CXCL8
Ser28-Ser99
Accession # P10145

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

THP‑1 human acute monocytic leukemia cell line treated (+) with PMA and LPS

Simple Western

25 µg/mL

THP‑1 human acute monocytic leukemia cell line treated with PMA and LPS and PBMC continioned media treated with LPS

Immunohistochemistry

5-15 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.1-0.5 µg/mL in the presence of 20 ng/mL Recombinant Human IL-8/CXCL8.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL-8/CXCL8 by Western Blot. Western blot shows lysates and conditioned media of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA and 10 ug/mL LPS for 24 hours and 3 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of Human IL-8/CXCL8 by Simple Western^TM. Simple Western lane view shows PBMC conditioned media untreated (-) or treated (+) with 1 μg/mL LPS for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for IL-8/CXCL8 at approximately 8 kDa (as indicated) using 25 µg/mL of Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Simple Western

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Detection of Human IL-8/CXCL8 by Simple Western^TM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hrs and 10ug/mL LPS for 3 hrs, loaded at 0.2 mg/mL. A specific band was detected for IL-8/CXCL8 at approximately 8 kDa (as indicated) using 25 µg/mL of Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Neutralization

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Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody. Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA). The ND₅₀ is typically 0.1-0.5 µg/mL.

Immunocytochemistry

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IL-8/CXCL8 in Human PBMCs. IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry

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IL-8/CXCL8 in Human Tonsil. IL-8/CXCL8 was detected in immersion fixed paraffin-embedded sections of human tonsil (surgically removed due to severe EBV-induced mononucleosis) using Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑208‑NA) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-8/CXCL8

CXCL8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.

CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivo and in vitro.

Long Name

Interleukin 8

Entrez Gene IDs

3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)

Alternate Names

3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human IL-8/CXCL8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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High interleukin‑8 and/or extracellular signal‑regulated kinase 2 expression predicts poor prognosis in patients with hepatocellular carcinoma
Authors: Youyuan Deng, Zhijie Ning, Zhiya Hu, Qianle Yu, Bin He, Guohuang Hu
Oncology Letters
NF-kappa B non-cell-autonomously regulates cancer stem cell populations in the basal-like breast cancer subtype
Authors: Mizuki Yamamoto, Yuu Taguchi, Taku Ito-Kureha, Kentaro Semba, Noritaka Yamaguchi, Jun-ichiro Inoue
Nature Communications
Human pituitary tumor-transforming gene 1 overexpression reinforces oncogene-induced senescence through CXCR2/p21 signaling in breast cancer cells
Authors: Jhen-Wei Ruan, Yi-Chu Liao, Ingrid Lua, Ming-Hsun Li, Chih-Yi Hsu, Ji-Hshiung Chen
Breast Cancer Research
Chemokines and chemokine receptors expression in the lesions of patients with American cutaneous leishmaniasis
Authors: Nilka Luisa Díaz, Olga Zerpa, Félix Jacobo Tapia
Mem. Inst. Oswaldo Cruz
A positive feedback loop between gastric cancer cells and tumor-associated macrophage induces malignancy progression
Authors: Haiyan Piao, Lingfeng Fu, Yuxin Wang, Yang Liu, Yue Wang, Xiangyu Meng et al.
Journal of Experimental & Clinical Cancer Research
Therapeutic potency of compound RMY-205 for pulmonary fibrosis induced by SARS-CoV-2 nucleocapsid protein
Authors: Zhi-yuan Zhang, Cui-yu Ju, Liu-zheng Wu, Han Yan, Wen-bin Hong, Hang-zi Chen et al.
Cell Chemical Biology
Characterization of Innate Immunity in an Extended Whole Blood Model of Human Islet Allotransplantation
Authors: Maria Hårdstedt, Susanne Lindblom, Alex Karlsson-Parra, Bo Nilsson, Olle Korsgren
Cell Transplantation
2-Methoxyestradiol-3,17-O,O-bis-sulfamate (STX140) Inhibits Proliferation and Invasion via Senescence Pathway Induction in Human BRAFi-Resistant Melanoma Cells
Authors: Franco, YA;de Moraes, MO;Carvalho, LAC;Dohle, W;da Silva, RO;Noma, IHY;Lima, K;Potter, BVL;Machado-Neto, JA;Maria-Engler, SS;
International journal of molecular sciences
Species: Human
Sample Types:
Exosomal IL-8 derived from Lung Cancer and Colon Cancer cells induced adipocyte atrophy via NF-kappaB signaling pathway
Authors: H Xiong, J Ye, K Xie, W Hu, N Xu, H Yang
Lipids in Health and Disease, 2022-12-29;21(1):147.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line
Authors: P Ortiz-Mont, A Londoño-Va, JP Vernot
Cell Commun. Signal, 2017-05-04;15(1):17.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Cigarette smoking increases bronchial mucosal IL-17A expression in asthmatics, which acts in concert with environmental aeroallergens to engender neutrophilic inflammation
Authors: LQ Siew, SY Wu, S Ying, CJ Corrigan
Clin. Exp. Allergy, 2017-03-13;0(0):.
Species: Human
Sample Types: Tissue Homogenates
Applications: IHC

Necrotic cells influence migration and invasion of glioblastoma via NF-?B/AP-1-mediated IL-8 regulation
Authors: SH Ahn, H Park, YH Ahn, S Kim, MS Cho, JL Kang, YH Choi
Sci Rep, 2016-04-14;6(0):24552.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Neuroblastoma-derived TGF-beta1 modulates the chemokine receptor repertoire of human resting NK cells.
Authors: Castriconi R, Dondero A, Bellora F, Moretta L, Castellano A, Locatelli F, Corrias M, Moretta A, Bottino C
J Immunol, 2013-04-10;190(10):5321-8.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

The presence of a matrix-derived neutrophil chemoattractant in bronchiolitis obliterans syndrome after lung transplantation.
Authors: Hardison MT, Galin FS, Calderon CE, Djekic UV, Parker SB, Wille KM, Jackson PL, Oster RA, Young KR, Blalock JE, Gaggar A
J. Immunol., 2009-04-01;182(7):4423-31.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Estradiol increases IL-8 secretion of normal human breast tissue and breast cancer in vivo.
Authors: Bendrik C, Dabrosin C
J. Immunol., 2009-01-01;182(1):371-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Functional characterization of IL-17F as a selective neutrophil attractant in psoriasis.
Authors: Watanabe H, Kawaguchi M, Fujishima S, Ogura M, Matsukura S, Takeuchi H, Ohba M, Sueki H, Kokubu F, Hizawa N, Adachi M, Huang SK, Iijima M
J. Invest. Dermatol., 2008-10-02;129(3):650-6.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
Photochem. Photobiol., 2004-09-01;80(2):231-5.
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
Authors: Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
J. Virol., 2003-02-01;77(3):1727-37.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Neutralization

Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
J. Immunol., 2002-12-01;169(11):6515-21.
Species: Human
Sample Types: BALF, Whole Tissue
Applications: ELISA Development, IHC-P

Isolation methodology is essential to the evaluation of the extracellular vesicle component of the senescence‐associated secretory phenotype
Authors: Ryan Wallis, Natasa Josipovic, Hannah Mizen, Arturo Robles‐Tenorio, Eleanor J. Tyler, Argyris Papantonis et al.
Journal of Extracellular Vesicles

Assessment of chemokine profiles in human skin biopsies by an immunoaffinity capillary electrophoresis chip
Authors: Heather Kalish, Terry M. Phillips
Methods

Interleukin-17 induces angiogenesis inï¿½vitro via CXCL8 and CCL2 in retinal pigment epithelium
Authors: Y Chen, M Zhong, G Yuan, H Peng
Mol Med Rep, 2018-01-18;0(0):.

Interleukin-8 Can Reduce Post-Surgical Lymphedema Formation by Promoting Lymphatic Vessel Regeneration
Authors: Inho Choi, Yong Suk Lee, Hee Kyoung Chung, Dongwon Choi, Tatiana Ecoiffier, Ha Neul Lee et al.
Angiogenesis

FAQs

Are mouse or rat CXCL8/IL-8 products offered?
- Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.

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