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Human IL-8/CXCL8 Antibody

Catalog #: AB-208-NA
13 Citations Datasheet
Catalog # Availability Size / Price Qty
AB-208-NA
Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody.
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Citations (13)
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Human IL-8/CXCL8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) GRO alpha, rhGRO beta, and rhGRO gamma is observed, and less than 1% cross-reactivity with recombinant feline IL-8/CXCL8, recombinant porcine IL-8/CXCL8, and recombinant canine IL-8/CXCL8 is observed.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human IL-8/CXCL8
Ser28-Ser99
Accession # P10145
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL-8/CXCL8 (Catalog # 208-IL)
Neutralization
Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 20 ng/mL Recombinant Human IL-8/CXCL8.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody. View Larger

Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody. Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-8/CXCL8 Polyclonal Antibody (Catalog # AB-208-NA). The ND50 is typically 5-20 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-8/CXCL8

CXCL8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.

CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivo and in vitro.

Long Name
Interleukin 8
Entrez Gene IDs
3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names
3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

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Citations for Human IL-8/CXCL8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Inflammatory and Proliferative Pathway Activation in Human Esophageal Myofibroblasts Treated with Acidic Bile Salts
    Authors: M Patankar, M Li, A Khalatbari, JD Castle, L Hu, C Zhang, A Shaker
    International Journal of Molecular Sciences, 2022-09-08;23(18):.
    Species: Human
    Sample Types: Cell Culture Supernatants
  2. PERK signaling through C/EBPdelta contributes to ER stress-induced expression of immunomodulatory and tumor promoting chemokines by cancer cells
    Authors: N Sheshadri, DK Poria, S Sharan, Y Hu, C Yan, VN Koparde, K Balamuruga, E Sterneck
    Cell Death & Disease, 2021-11-01;12(11):1038.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  3. Directed evolution of broadly crossreactive chemokine-blocking antibodies efficacious in arthritis
    Authors: A Angelini, Y Miyabe, D Newsted, BH Kwan, C Miyabe, RL Kelly, MN Jamy, AD Luster, KD Wittrup
    Nat Commun, 2018-04-13;9(1):1461.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Salmonella enteritidis Effector AvrA Stabilizes Intestinal Tight Junctions via the JNK Pathway
    Authors: Zhijie Lin
    J. Biol. Chem, 2016-11-15;291(52):26837-26849.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  5. Evidence for chemokine synergy during neutrophil migration in ARDS
    Thorax, 2016-08-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Ultratrace level determination and quantitative analysis of kidney injury biomarkers in patient samples attained by zinc oxide nanorods
    Authors: Manpreet Singh, Anginelle Alabanza, Lorelis E. Gonzalez, Weiwei Wang, W. Brian Reeves, Jong-in Hahm
    Nanoscale
  7. Autocrine signaling is a key regulatory element during osteoclastogenesis
    Authors: Paul Kopesky, Kerstin Tiedemann, Dahlia Alkekhia, Christoph Zechner, Bjorn Millard, Birgit Schoeberl et al.
    Biology Open
  8. Autoantibodies to angiotensin and endothelin receptors in systemic sclerosis induce cellular and systemic events associated with disease pathogenesis
    Authors: Angela Kill, Christoph Tabeling, Reinmar Undeutsch, Anja A Kühl, Jeannine Günther, Mislav Radic et al.
    Arthritis Research & Therapy
  9. Crimean-Congo Hemorrhagic Fever Virus-Infected Hepatocytes Induce ER-Stress and Apoptosis Crosstalk
    Authors: Raquel Rodrigues, Gláucia Paranhos-Baccalà, Guy Vernet, Christophe N. Peyrefitte
    PLoS ONE
  10. Expression and regulation of chemokines in murine and human type 1 diabetes.
    Authors: Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D
    Diabetes, 2011-12-30;61(2):436-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  11. Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
    Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E
    Nat. Med., 2011-10-30;17(11):1498-503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Tumor-derived chemokine MCP-1/CCL2 is sufficient for mediating tumor tropism of adoptively transferred T cells.
    Authors: Brown CE, Vishwanath RP, Aguilar B, Starr R, Najbauer J, Aboody KS, Jensen MC
    J. Immunol., 2007-09-01;179(5):3332-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. The effect of respiratory synctial virus on chemokine release by differentiated airway epithelium.
    Authors: Mellow TE, Murphy PC, Carson JL, Noah TL, Zhang L, Pickles RJ
    Exp. Lung Res., 2004-01-01;30(1):43-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

  1. Are mouse or rat CXCL8/IL-8 products offered?

    • Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.

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