Human IL-7 Antibody

Catalog #: AF-207-NA
4 Citations 3 Reviews Datasheet

Catalog #	Availability	Size / Price	Qty
AF-207-NA
AF-207-SP

Detection of Recombinant Human and Mouse IL‑7 by Western Blot.

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All IL-7 Antibodies

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Citations (4)

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Reviews (3)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-7 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IL-7
Asp26-His177
Accession # P13232

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Neutralization

Measured by its ability to neutralize IL‑7-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The Neutralization Dose (ND₅₀) is typically ≤ 0.5 µg/mL in the presence of 2.5 ng/mL Recombinant Human IL‑7.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Recombinant Human and Mouse IL‑7 by Western Blot. Western blot shows 25 ng of Recombinant Human IL-7 (207-IL), Recombinant Mouse IL-7 (407-ML) and Recombinant Rat IL-7 (7857-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody HAF109). A specific band was detected for IL-7 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Neutralization

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Cell Proliferation Induced by IL‑7 and Neutralization by Human IL‑7 Antibody. Recombinant Human IL-7 (207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA). The ND₅₀ is typically ≤ 0.5µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7

IL-7, previously known as pre-B cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4⁺ and CD8⁺ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumoricidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues.

Human IL-7 cDNA encodes a precursor protein of 177 amino residues containing a 25 amino acid residue signal peptide. Mouse IL-7 has approximately 65% amino acid sequence identity with human IL-7 and both proteins exhibit cross-species activity.

IL-7 bioactivites are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells and bone marrow cells.

Long Name

Interleukin 7

Entrez Gene IDs

3574 (Human); 16196 (Mouse); 25647 (Rat)

Alternate Names

IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human IL-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Interleukin 7 plays a role in T lymphocyte apoptosis inhibition driven by mesenchymal stem cell without favoring proliferation and cytokines secretion.
Authors: Normanton M, Alvarenga H, Hamerschlak N, Ribeiro A, Kondo A, Rizzo L, Marti L
PLoS ONE, 2014-09-03;9(9):e106673.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells.
Authors: Johnson SE, Shah N, Panoskaltsis-Mortari A, Lebien TW
J. Immunol., 2005-12-01;175(11):7325-31.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
J. Immunol., 2005-02-15;174(4):2376-86.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Structure function analysis of interleukin 7: requirement for an aromatic ring at position 143 of helix D.
Authors: vanderSpek JC, Sutherland JA, Gill BM, Gorgun G, Foss FM, Murphy JR
Cytokine, 2002-03-07;17(5):227-33.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot