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Human IL-4 Antibody

Catalog #: AF-204-NA
12 Citations Datasheet
Catalog # Availability Size / Price Qty
AF-204-NA
AF-204-SP
Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody.
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Citations (12)
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Human IL-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-4 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL‑4
His25-Ser153
Accession # NP_000580
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑4 (Catalog # 204-IL)
Neutralization
Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.02-0.1µg/mL in the presence of 0.5 ng/mL Recombinant Human IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody. View Larger

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody. Recombinant Human IL-4 (204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-204-NA). The ND50 is typically 0.02-0.1 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure. Human IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities. IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma.

Long Name
Interleukin 4
Entrez Gene IDs
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

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Citations for Human IL-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. A Patient with Erythema Nodosus Leprosum and Chagas Cardiopathy: Challenges in Patient Management and Review of the Literature
    Authors: Maria Ângela B. Trindade, Noemia B Carvalho, Maria A. Shikanai-Yasuda, Erika Pagliari, Noemia B. Carvalho, Neusa Y Sakai-Valente et al.
    The American Journal of Tropical Medicine and Hygiene
  2. Severe Leptospirosis Features in the Spleen Indicate Cellular Immunosuppression Similar to That Found in Septic Shock
    Authors: Amaro Nunes Duarte-Neto, Julio Croda, Carla Pagliari, Francisco Garcia Soriano, Antonio Carlos Nicodemo, Maria Irma Seixas Duarte
    Frontiers in Immunology
  3. Humoral and Cellular Immune Responses Induced by Bivalent DNA Vaccines Expressing Fusion Capsid Proteins of Porcine Circovirus Genotypes 2a and 2b
    Authors: Meas, S;Chaimongkolnukul, K;Narkpuk, J;Mekvichitsaeng, P;Poomputsa, K;Wanasen, N;Roshorm, YM;
    Vaccines
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Western Blot, Immunocytochemistry
  4. Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice
    Authors: E Conde, R Bertrand, B Balbino, J Bonnefoy, J Stackowicz, N Caillot, F Colaone, S Hamdi, R Houmadi, A Loste, JBJ Kamphuis, F Huetz, L Guillemina, N Gaudenzio, A Mougel, D Hardy, JN Snouwaert, BH Koller, V Serra, P Bruhns, G Grouard-Vo, LL Reber
    Nature Communications, 2021-05-11;12(1):2574.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Western Blot
  5. Inhibition of G-protein betagamma signaling enhances T cell receptor-stimulated interleukin 2 transcription in CD4+ T helper cells.
    Authors: Yost E, Hynes T, Hartle C, Ott B, Berlot C
    PLoS ONE, 2015-01-28;10(1):e0116575.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
    Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
    Blood, 2010-06-10;116(12):2061-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
    Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK
    Immunol. Lett., 2009-11-04;127(2):150-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008-04-01;38(4):1012-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
    Authors: Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC
    Blood, 2007-02-13;109(11):4856-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Peripheral expansion of circulating T-helper 1 cells predicts coronary endothelial dysfunction after cardiac transplantation.
    Authors: Methe H, Wiegand D, Welsch U, Meiser B, Nabauer M, Koglin J
    J. Heart Lung Transplant., 2005-07-01;24(7):833-40.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression.
    Authors: Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G
    Eur. J. Immunol., 2004-12-01;34(12):3485-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
    Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
    J. Allergy Clin. Immunol., 2002-10-01;110(4):624-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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