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Human IL-4 Antibody

Catalog #: MAB204
27 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
MAB204-SP
MAB204-500
MAB204-100
Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody.
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Product Details
Citations (27)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse IL‑4, recombinant porcine IL‑4, recombinant cotton rat IL‑4, recombinant rat IL‑4, or recombinant human IL‑13 is observed.
Source
Monoclonal Mouse IgG2B Clone # 34019
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL‑4
His25-Ser153
Accession # P05112
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑4 (Catalog # 204-IL)
Neutralization
Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 0.5 ng/mL Recombinant Human IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody. View Larger

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody. Recombinant Human IL-4 (Catalog # 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB204). The ND50 is typically 0.5-1.5 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

References
  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  14. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name
Interleukin 4
Entrez Gene IDs
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

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Citations for Human IL-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. RNA-sequencing and mass-spectrometry proteomic time-series analysis of T-cell differentiation identified multiple splice variants models that predicted validated protein biomarkers in inflammatory diseases
    Authors: Rasmus Magnusson, Olof Rundquist, Min Jung Kim, Sandra Hellberg, Chan Hyun Na, Mikael Benson et al.
    Frontiers in Molecular Biosciences
  2. The Cytokine IL-17A Limits Th17 Pathogenicity via a Negative Feedback Loop Driven by Autocrine Induction of IL-24
    Authors: Wai Po Chong, Mary J. Mattapallil, Kumarkrishna Raychaudhuri, So Jin Bing, Sihan Wu, Yajie Zhong et al.
    Immunity
  3. Long Intergenic Noncoding RNA MIAT as a Regulator of Human Th17 Cell Differentiation
    Authors: Mohd Moin Khan, Meraj Hasan Khan, Ubaid Ullah Kalim, Sofia Khan, Sini Junttila, Niklas Paulin et al.
    Frontiers in Immunology
  4. A dynamic single cell-based framework for digital twins to prioritize disease genes and drug targets
    Authors: Xinxiu Li, Eun Jung Lee, Sandra Lilja, Joseph Loscalzo, Samuel Schäfer, Martin Smelik et al.
    Genome Medicine
  5. Bilirubin suppresses Th17 immunity in colitis by upregulating CD39
    Authors: MS Longhi, M Vuerich, A Kalbasi, JE Kenison, A Yeste, E Csizmadia, B Vaughn, L Feldbrugge, S Mitsuhashi, B Wegiel, L Otterbein, A Moss, FJ Quintana, SC Robson
    JCI Insight, 2017-05-04;2(9):.
  6. Vitamin D Counteracts Mycobacterium tuberculosis-Induced Cathelicidin Downregulation in Dendritic Cells and Allows Th1 Differentiation and IFN gamma Secretion
    Authors: Anna K. O. Rode, Martin Kongsbak, Marie M. Hansen, Daniel Villalba Lopez, Trine B. Levring, Anders Woetmann et al.
    Frontiers in Immunology
  7. Multi-omics protein-coding units as massively parallel Bayesian networks: Empirical validation of causality structure
    Authors: Alberto Zenere, Olof Rundquist, Mika Gustafsson, Claudio Altafini
    iScience
  8. Interferon subverts an AHR-JUN axis to promote CXCL13+ T cells in lupus
    Authors: Law, C;Wacleche, VS;Cao, Y;Pillai, A;Sowerby, J;Hancock, B;Horisberger, A;Bracero, S;Skidanova, V;Li, Z;Adejoorin, I;Dillon, E;Benque, IJ;Nunez, DP;Simmons, DP;Keegan, J;Chen, L;Baker, T;Brohawn, PZ;Al-Mossawi, H;Hao, LY;Jones, B;Rao, N;Qu, Y;Alves, SE;Accelerating Medicines Partnership: RA/SLE Network, ;Jonsson, AH;Shaw, KS;Vleugels, RA;Massarotti, E;Costenbader, KH;Brenner, MB;Lederer, JA;Hultquist, JF;Choi, J;Rao, DA;
    Nature
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Anti-Inflammatory Effect of Specialized Proresolving Lipid Mediators on Mesenchymal Stem Cells: An In Vitro Study
    Authors: S AlZahrani, Z Shinwari, A Gaafar, A Alaiya, A Al-Kahtani
    Cells, 2022-12-28;12(1):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Induced Human Regulatory T Cells Express the Glucagon-like Peptide-1 Receptor
    Authors: AKO Rode, TB Buus, V Mraz, FAH Al-Jaberi, DV Lopez, SL Ford, S Hennen, IP Eliasen, IV Klewe, L Gharehdagh, A Dragan, MM Rosenkilde, A Woetmann, L Skov, N Ødum, CM Bonefeld, M Kongsbak-W, C Geisler
    Cells, 2022-08-19;11(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Stimulation
  11. IFNgamma-primed periodontal ligament cells regulate T-cell responses via IFNgamma-inducible mediators and ICAM-1-mediated direct cell contact
    Authors: W Singhatana, S Kitpakorns, M Toso, P Pavasant
    Royal Society open science, 2022-07-27;9(7):220056.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells.
    Authors: Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B
    iScience, 2021-11-14;24(12):103433.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Early-Life Stress Regulates Cardiac Development through an IL-4-Glucocorticoid Signaling Balance
    Authors: DC Apaydin, PAM Jaramillo, L Corradi, F Cosco, FG Rathjen, T Kammertoen, A Filosa, S Sawamiphak
    Cell Rep, 2020-11-17;33(7):108404.
    Species: Zebrafish
    Sample Types: In Vivo
    Applications: In Vivo
  14. Melatonin ameliorates necrotizing enterocolitis by preventing Th17/Treg imbalance through activation of the AMPK/SIRT1 pathway
    Authors: F Ma, H Hao, X Gao, Y Cai, J Zhou, P Liang, J Lv, Q He, C Shi, D Hu, B Chen, L Zhu, X Xiao, S Li
    Theranostics, 2020-06-19;10(17):7730-7746.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Homeostatic Milieu Induces Production of Deoxyribonuclease 1-like 3 from Myeloid Cells
    Authors: S Inokuchi, H Mitoma, S Kawano, S Nakano, M Ayano, Y Kimoto, M Akahoshi, Y Arinobu, H Tsukamoto, K Akashi, T Horiuchi, H Niiro
    J. Immunol., 2020-03-18;204(8):2088-2097.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Urothelial bladder cancer may suppress perforin expression in CD8+ T cells by an ICAM-1/TGF?2 mediated pathway
    Authors: CA Hartana, E Ahlén Berg, AA Zirakzadeh, D Krantz, ME Winerdal, M Winerdal, M Johansson, F Alamdari, T Jakubczyk, H Glise, K Riklund, A Sherif, O Winqvist
    PLoS ONE, 2018-07-02;13(7):e0200079.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Vitamin D up-regulates the vitamin D receptor by protecting it from proteasomal degradation in human CD4+ T cells.
    Authors: Kongsbak M, von Essen M, Boding L, Levring T, Schjerling P, Lauritsen J, Woetmann A, Odum N, Bonefeld C, Geisler C
    PLoS ONE, 2014-05-02;9(5):e96695.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Impaired development of human Th1 cells in patients with deficient expression of STAT4.
    Authors: Chang HC, Han L, Goswami R, Nguyen ET, Pelloso D, Robertson MJ, Kaplan MH
    Blood, 2009-04-09;113(23):5887-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation, Differentiation
  19. Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus.
    Authors: Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V
    J. Exp. Med., 2009-03-16;206(4):793-805.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  20. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
    Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2007-08-05;8(9):942-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006-08-17;241(1):7-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006-06-16;120(2):220-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Cytokine milieu of atopic dermatitis skin subverts the innate immune response to vaccinia virus.
    Authors: Howell MD, Gallo RL, Boguniewicz M, Jones JF, Wong C, Streib JE, Leung DY
    Immunity, 2006-03-01;24(3):341-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Cutting edge: immune cells as sources and targets of the IL-10 family members?
    Authors: Wolk K, Kunz S, Asadullah K, Sabat R
    J. Immunol., 2002-06-01;168(11):5397-402.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  25. Progesterone Inhibits the Establishment of Activation-Associated Chromatin During TH1 Differentiation
    Authors: Olof Rundquist, Colm E. Nestor, Maria C. Jenmalm, Sandra Hellberg, Mika Gustafsson
    Frontiers in Immunology
  26. Protein interactome of the Cancerous Inhibitor of protein phosphatase 2A (CIP2A) in Th17 cells
    Authors: Mohd Moin Khan, Tommi Välikangas, Meraj Hasan Khan, Robert Moulder, Ubaid Ullah, Santosh Dilip Bhosale et al.
    Current Research in Immunology
  27. Preclinical Characterization of GLPG0634, a Selective Inhibitor of JAK1, for the Treatment of Inflammatory Diseases
    Authors: Luc Van Rompaey, René Galien, Ellen M. van der Aar, Philippe Clement-Lacroix, Luc Nelles, Bart Smets et al.
    The Journal of Immunology

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Human IL-4 Antibody
By Anonymous on 12/06/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Microarray Human IL-4 Antibody MAB204
Human IL-4 Antibody
By Priyank raje on 12/03/2017
Application: ELISA Sample Tested: T cells Species: Human and Rat