Human IL-23 p19 Antibody Summary
Arg20-Pro189
Accession # Q9NPF7
Applications
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human EBI3 Monoclonal Antibody (Catalog # MAB64561).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human IL‑23 p19 by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line transfected with human IL-23. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-23 p19 Monoclonal Antibody (Catalog # MAB17161) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-23 p19 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Human IL‑39 ELISA Standard Curve. Recombinant Human IL‑39 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL‑23 p19 Monoclonal Antibody (Catalog # MAB17161) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human EBI3 Monoclonal Antibody (MAB64561) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-23
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.
- Oppmann, B. et al. (2000) Immunity 13:715.
- Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
- Parham, C. et al. (2002) J. Immunol. 168:5699.
- Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
- Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
Product Datasheets
Citations for Human IL-23 p19 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Functional analysis of human circulating immune cells based on high-dimensional mass cytometry
Authors: Xiuxing Liu, Jianjie Lv, Huishi Wang, Yingfeng Zheng, Wenru Su
STAR Protocols
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Inherited PD-1 deficiency underlies tuberculosis and autoimmunity in a child
Authors: M Ogishi, R Yang, C Aytekin, D Langlais, M Bourgey, T Khan, FA Ali, M Rahman, OM Delmonte, M Chrabieh, P Zhang, C Gruber, SJ Pelham, AN Spaan, J Rosain, WT Lei, S Drutman, MD Hellmann, MK Callahan, M Adamow, P Wong, JD Wolchok, G Rao, CS Ma, Y Nakajima, T Yaguchi, K Chamoto, SC Williams, JF Emile, F Rozenberg, MS Glickman, F Rapaport, G Kerner, G Allington, I Tezcan, D Cagdas, FO Hosnut, F Dogu, A Ikinciogul, VK Rao, L Kainulaine, V Béziat, J Bustamante, S Vilarinho, RP Lifton, B Boisson, L Abel, D Bogunovic, N Marr, LD Notarangel, SG Tangye, T Honjo, P Gros, S Boisson-Du, JL Casanova
Nature Medicine, 2021-06-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Monocyte-derived dendritic cells from Crohn's disease patients exhibit decreased ability to activate T helper type 17 responses in memory cells.
Authors: Nieminen J, Sipponen T, Farkkila M, Vaarala O
Clin Exp Immunol, 2014-07-01;177(1):190-202.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Induction and activation of human Th17 by targeting antigens to dendritic cells via dectin-1.
Authors: Duluc D, Joo H, Ni L, Yin W, Upchurch K, Li D, Xue Y, Klucar P, Zurawski S, Zurawski G, Oh S
J Immunol, 2014-05-16;192(12):5776-88.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, Functional Assay
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