Human IL-23 p19 Antibody

Catalog #: MAB12901 Datasheet

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB12901-100
MAB12901-SP

Detection of Human IL‑23 p19 by Western Blot.

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Human IL-23 p19 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-23 p19 in direct ELISAs and Western blots.

Source

Recombinant Monoclonal Rabbit IgG_2B Clone # 1193B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human IL-23 p19
Arg20-Pro189
Accession # Q9NPF7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Neutralization

Measured by its ability to neutralize IL‑23-induced IL‑17 secretion in mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The Neutralization Dose (ND₅₀) is typically 0.15-0.9 µg/mL in the presence of 0.3 ng/mL Recombinant Human IL‑23.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL‑23 p19 by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with human IL-23 p19. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human IL-23 p19 Monoclonal Antibody (Catalog # MAB12901) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IL-23 p19 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization

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IL‑17 Secretion Induced by IL‑23 and Neutralization by Human IL‑23 Antibody. Recombinant Human IL-23 (Catalog # 1290-IL) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-23 p19 Monoclonal Antibody (Catalog # MAB1290). The ND₅₀ is typically 0.15-0.9 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Human p19 cDNA encodes a 189 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 170 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8^- and CD8⁺ subtypes, however only IL-23 can act directly on CD8⁺ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

References

Oppmann, B. et al. (2000) Immunity 13:715.
Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
Parham, C. et al. (2002) J. Immunol. 168:5699.
Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Long Name

Interleukin 23

Entrez Gene IDs

51561 (Human); 83430 (Mouse); 155140 (Rat); 102128555 (Cynomolgus Monkey)

Alternate Names

IL-23 p19/IL-12 p40; IL23; IL-23; IL-23A; IL-23-A; IL-23p19; IL-23p19/IL-12p40; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha