Human IL-1ra/IL-1F3 Antibody

Catalog #: AF-280-NA
12 Citations 2 Reviews Datasheet

Catalog #	Availability	Size / Price	Qty
AF-280-NA
AF-280-SP

Detection of Human IL‑1ra/IL‑1F3 by Western Blot.

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All IL-1ra/IL-1F3 Antibodies

Product Details

Citations (12)

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Supplemental Products

Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-1ra/IL-1F3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑1ra/IL-1F3 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 15% cross-reactivity with recombinant mouse IL-1ra is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IL‑1ra/IL-1F3
Arg26-Glu177
Accession # P18510

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

20 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize IL‑1ra/IL‑1F3 inhibition of IL‑1 alpha /IL‑1F1-dependent proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically ≤4 µg/mL in the presence of 50 ng/mL Recombinant Human IL‑1ra/IL‑1F3 and 50 pg/mL Recombinant Human IL‑1 alpha /IL‑1F1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL‑1ra/IL‑1F3 by Western Blot. Western blot shows lysates of RT-4 human bladder carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1ra/IL-1F3 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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IL‑1ra/IL‑1F3 in Human Skin. IL-1ra/IL-1F3 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human and Mouse IL‑1ra/IL‑1F3 by Simple Western^TM. Simple Western lane view shows lysates of RT-4 human bladder carcinoma cell line, A431 human epithelial carcinoma cell line, human skin cancer tissue, and mouse skin cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for IL-1ra/IL-1F3 at approximately 24-26 kDa (as indicated) using 20 µg/mL of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Neutralization

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IL‑1ra/IL‑1F3 Inhibition of IL‑1 alpha /IL‑1F1-dependent Cell Proliferation and Neutralization by Human IL‑1ra/IL‑1F3 Antibody. Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA). The ND₅₀ is typically ≤ 4 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1ra/IL-1F3

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

Long Name

Interleukin 1 Receptor Antagonist

Entrez Gene IDs

3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)

Alternate Names

DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human IL-1ra/IL-1F3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Islet Interleukin-1beta Immunoreactivity Is an Early Feature of Cystic Fibrosis That May Contribute to beta-Cell Failure.
Authors: Hull RL, Gibson RL, McNamara S et al.
Diabetes Care
Interleukin-1?/Interleukin (IL)-1-Receptor-Antagonist (IL1-RA) Axis in Invasive Bladder Cancer-An Exploratory Analysis of Clinical and Tumor Biological Significance
Authors: Vukovic, M;Chamlati, JM;Hennenlotter, J;Todenhöfer, T;Lütfrenk, T;Jersinovic, S;Tsaur, I;Stenzl, A;Rausch, S;
International journal of molecular sciences
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Interleukin 1&beta/1RA axis in colorectal cancer regulates tumor invasion, proliferation and apoptosis via autophagy
Authors: Y Chen, Z Yang, B Deng, D Wu, Y Quan, Z Min
Oncol. Rep., 2020-01-22;43(3):908-918.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Prostaglandin E2 Indicates Therapeutic Efficacy Of Mesenchymal Stem Cells In Experimental Traumatic Brain Injury
Authors: DJ Kota, KS Prabhakara, N Toledano-F, D Bhattarai, Q Chen, B DiCarlo, P Smith, F Triolo, PL Wenzel, CS Cox, SD Olson
Stem Cells, 2017-03-16;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Intranasal Administration of Interleukin-1 Receptor Antagonist in a Transient Focal Cerebral Ischemia Rat Model
Authors: JH Lee, EH Kam, JM Kim, SY Kim, EJ Kim, SY Cheon, BN Koo
Biomol Ther (Seoul), 2017-03-01;25(2):149-157.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC
IL-1 Receptor Antagonist Chimeric Protein: Context-Specific and Inflammation-Restricted Activation.
Authors: Rider P, Carmi Y, Yossef R, Guttman O, Eini H, Azam T, Dinarello C, Lewis E
J Immunol, 2015-07-08;195(4):1705-12.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
IL-4 enhances IFN-lambda1 (IL-29) production by plasmacytoid DCs via monocyte secretion of IL-1Ra.
Authors: Megjugorac NJ, Gallagher GE, Gallagher G
Blood, 2010-03-16;115(21):4185-90.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
Dig. Dis. Sci., 2007-03-30;52(10):2775-83.
Species: Human
Sample Types: Whole Cells
Applications: ICC
P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
Authors: Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC
Br. J. Pharmacol., 2007-03-12;151(1):115-27.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
Immunolocalization of interleukin-1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies.
Authors: Grundtman C, Salomonsson S, Dorph C, Bruton J, Andersson U, Lundberg IE
Arthritis Rheum., 2007-02-01;56(2):674-87.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr

IL-22 increases the innate immunity of tissues.
Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
Immunity, 2004-08-01;21(2):241-54.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
J. Allergy Clin. Immunol., 2002-10-01;110(4):624-33.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization