Human IL-1 alpha /IL-1F1 (Research Grade Bermekimab Biosimilar) Antibody

Catalog #: MAB10568 Datasheet

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB10568-100
MAB10568-SP

Detection of IL‑1 alpha /IL‑1F1 (Bermekimab) in LPS-treated Human PBMCs by Flow Cytometry.

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Human IL-1 alpha /IL-1F1 (Research Grade Bermekimab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑1 alpha /IL‑1F1 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Bermekimab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu146

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human IL‑1 alpha /IL‑1F1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

LPS-treated Human PBMC

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

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Detection of IL‑1 alpha /IL‑1F1 (Bermekimab) in LPS-treated Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were (A) treated overnight with 1 µg/mL LPS or (B) untreated, then stained with Human IL-1a/IL-1F1 (Bermekimab) Monoclonal Antibody (Catalog # MAB10568, filled histogram) or unstained (open histogram), followed by Anti-Human IgG Allophycoerythrin-conjugated Secondary Antibody (F0135). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 alpha/IL-1F1

Research grade Bermekimab is a biosimilar that binds and blocks binds to IL-1 alpha/IL-1F1. This product can be used in flow cytometry or various assay formats to block or measure IL-1 alpha/IL-1F1

Long Name

Interleukin 1 alpha

Entrez Gene IDs

3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)

Alternate Names

BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha

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