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Human IL-17/IL-17A PE-conjugated Antibody

Catalog #: IC317P
1 Citation Datasheet
Catalog # Availability Size / Price Qty
IC317P
Detection of IL‑17/IL‑17A in Th17-differentiated Human CD4+PBMCs by Flow Cytometry.
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Human IL-17/IL-17A PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17/IL-17A in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17/IL-17A is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17/IL-17A, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
Source
Monoclonal Mouse IgG2B Clone # 41809
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human IL-17/IL-17A
Ile20-Ala155
Accession # Q16552
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-17/IL-17A antibody in Th17-differentiated Human CD4<SUP>+</SUP>PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑17/IL‑17A in Th17-differentiated Human CD4+PBMCs by Flow Cytometry. Human CD4+peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 50 ng/mL PMA, 200 ng/mL Calcium Ionomycin, 200 ng/mL LPS, 20 ng/mL Recombinant Human IL-23 (Catalog # 1290-IL), and 40 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) overnight to induce Th17 development were stained with Mouse Anti-Human IL-17/IL-17A PE-conjugated Monoclonal Antibody (Catalog # IC317P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17/IL-17A

IL-17/IL-17A is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.

Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

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Citation for Human IL-17/IL-17A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Enhancing the toolbox to study IL-17A in cattle and sheep
    Authors: SR Wattegeder, Y Corripio-M, Y Pang, D Frew, TN McNeilly, J Palarea-Al, CJ McInnes, JC Hope, EJ Glass, G Entrican
    Vet. Res., 2017-04-08;48(1):20.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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