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Human IL-17/IL-17A APC-conjugated Antibody

Catalog #: IC3171A
5 Citations Datasheet
Catalog # Availability Size / Price Qty
IC3171A-100
IC3171A-025
Detection of IL‑17/IL‑17A in Human PBMCs by Flow Cytometry.
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Citations (5)
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Human IL-17/IL-17A APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17 in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, recombinant canine Il-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
Source
Monoclonal Mouse IgG1 Clone # 41802
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-17/IL-17A antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑17/IL‑17A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA and Calcium Ionomycin were stained with Mouse Anti-Human IL-17/IL-17A APC-conjugated Monoclonal Antibody (Catalog # IC3171A) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17/IL-17A

Interleukin-17 (IL-17) is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.

Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

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Citations for Human IL-17/IL-17A APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. The Expression of IL-17, in Chronic Spontaneous Urticaria Is Linked to Semaphorin5A
    Authors: M Lobna, E Nasren, S Adi, B Jacob, GA Maria, M Marcus, V Zahava
    Biomolecules, 2021-03-02;11(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. TLR7/8 adjuvant overcomes newborn hyporesponsiveness to pneumococcal conjugate vaccine at birth
    Authors: DJ Dowling, SD van Haren, A Scheid, I Bergelson, D Kim, CJ Mancuso, W Foppen, A Ozonoff, L Fresh, TB Theriot, AA Lackner, RN Fichorova, D Smirnov, JP Vasilakos, JM Beaurline, MA Tomai, CC Midkiff, X Alvarez, JL Blanchard, MH Gilbert, PP Aye, O Levy
    JCI Insight, 2017-03-23;2(6):e91020.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Age-Specific Adjuvant Synergy: Dual TLR7/8 and Mincle Activation of Human Newborn Dendritic Cells Enables Th1 Polarization
    Authors: Ofer Levy
    J. Immunol., 2016-10-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Sunitinib indirectly enhanced anti-tumor cytotoxicity of cytokine-induced killer cells and CD3(+)CD56(+) subset through the co-culturing dendritic cells.
    Authors: Wongkajornsilp A, Wamanuttajinda V, Kasetsinsombat K, Duangsa-ard S, Sa-ngiamsuntorn K, Hongeng S, Maneechotesuwan K
    PLoS ONE, 2013-11-13;8(11):e78980.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. IL-21 and TGF-beta are required for differentiation of human T(H)17 cells.
    Authors: Yang L, Anderson DE, Baecher-Allan C, Hastings WD, Bettelli E, Oukka M, Kuchroo VK, Hafler DA
    Nature, 2008-05-11;454(7202):350-2.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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