Human IL-17/IL-17A Antibody Summary
Ile20-Ala155
Accession # Q16552
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human IL‑17 by Western Blot. Western blot shows lysates of human primary naïve CD4+ T cells, along with whole cell lysates (WCL) and conditioned-media supernatant (Supe) of human primary differentiated Th17 cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-17 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IL‑17/IL‑17A in Human Tonsil. IL-17/IL-17A was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-17/IL-17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Immunoprecipitation of Human IL‑17. Human IL-17 was immunoprecipitated from 100 µg of human primary differentiated Th17 cell lysate following incubation with 3 µg Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) or control antibody (AB-108-C) overnight at 4 °C. IL-17-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.
Detection of IL‑17 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca 2+ionomycin for 16 hours, then stained with Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA, dark orange filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody. Recombinant Human IL-17 (317-ILB) stimulates IL-6 secretion in NIH/3T3 mouse embryonic fibroblasts in a dose-dependent manner (orange line), as measured by the mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Human IL-17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). The ND50 is typically 0.02‑0.12 µg/mL.
Detection of Human IL-17/IL-17A by Immunohistochemistry Expression of psoriasis‐related cytokines in paradoxical psoriasiform reactions. Immunohistochemistry analysis performed on paradoxical skin lesions obtained from patients 1 (Pt1) and 2 (Pt2) shows similar values of IL‐17A+ cells, a reduction of dermal IFN‐ gamma + cells and an increase of IL‐22+ or IL‐36 gamma + cells, when compared with psoriatic skin lesions. LS and NLS skin of the same psoriatic patient (n = 3) was analyzed. Graphs show the mean of number of positive cells + SD per three sections. One out of three representative stainings is shown. *p < 0.01, **p < 0.05, versus classical psoriasis. Scale bars, 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31577850), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IL-17/IL-17A by Immunohistochemistry Inflammatory cell infiltrates of early acne lesions in immunohistochemistry.Representative immunohistochemical staining showing IL-17A+ cells in early acne lesion (a). Staining of IL-17A+ and CD3+ cells shows that IL-17A (in green) and CD3 (in red) were detected in acne vulgaris lesions. Cell nuclei were counterstained in blue. Arrows indicate IL-17A and CD3 double positive cells (b). T-bet+ cells (c) were more numerous than IL-17A+ cells or Foxp3+ cells (d) in the lesional acne skin. Large number of CD4+ cells (e), mostly lymphocytes, was seen around pilosebaceous unit and perivascularly. CD8+ cells (f) were fewer in number than CD4+ cells. Large number of CD68+ macrophages (g) and a few CD83+ cells (h), which are mature dendritic cells, were detected around sebaceous follicles. (Bar = 200 µm; in the insets bar = 50 µm). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25153527), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IL-17/IL-17A by Immunohistochemistry Inflammatory cell infiltrates of early acne lesions in immunohistochemistry.Representative immunohistochemical staining showing IL-17A+ cells in early acne lesion (a). Staining of IL-17A+ and CD3+ cells shows that IL-17A (in green) and CD3 (in red) were detected in acne vulgaris lesions. Cell nuclei were counterstained in blue. Arrows indicate IL-17A and CD3 double positive cells (b). T-bet+ cells (c) were more numerous than IL-17A+ cells or Foxp3+ cells (d) in the lesional acne skin. Large number of CD4+ cells (e), mostly lymphocytes, was seen around pilosebaceous unit and perivascularly. CD8+ cells (f) were fewer in number than CD4+ cells. Large number of CD68+ macrophages (g) and a few CD83+ cells (h), which are mature dendritic cells, were detected around sebaceous follicles. (Bar = 200 µm; in the insets bar = 50 µm). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25153527), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17/IL-17A
Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has recently been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation.
Product Datasheets
Citations for Human IL-17/IL-17A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance
Authors: Zheng N, Fang J, Xue G et al.
Cancer cell
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A systematic review and critical evaluation of immunohistochemical associations in hidradenitis suppurativa.
Authors: Frew John W, Hawkes Jason E, Krueger James G
F1000Research
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Sex-dependent APOE4 neutrophil-microglia interactions drive cognitive impairment in Alzheimer's disease
Authors: Rosenzweig, N;Kleemann, KL;Rust, T;Carpenter, M;Grucci, M;Aronchik, M;Brouwer, N;Valenbreder, I;Cooper-Hohn, J;Iyer, M;Krishnan, RK;Sivanathan, KN;Brandão, W;Yahya, T;Durao, A;Yin, Z;Chadarevian, JP;Properzi, MJ;Nowarski, R;Davtyan, H;Weiner, HL;Blurton-Jones, M;Yang, HS;Eggen, BJL;Sperling, RA;Butovsky, O;
Nature medicine
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
NF?B and NLRP3/NLRC4 inflammasomes regulate differentiation, activation and functional properties of monocytes in response to distinct SARS-CoV-2 proteins
Authors: Tsukalov, I;Sánchez-Cerrillo, I;Rajas, O;Avalos, E;Iturricastillo, G;Esparcia, L;Buzón, MJ;Genescà, M;Scagnetti, C;Popova, O;Martin-Cófreces, N;Calvet-Mirabent, M;Marcos-Jimenez, A;Martínez-Fleta, P;Delgado-Arévalo, C;de Los Santos, I;Muñoz-Calleja, C;Calzada, MJ;González Álvaro, I;Palacios-Calvo, J;Alfranca, A;Ancochea, J;Sánchez-Madrid, F;Martin-Gayo, E;
Nature communications
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Immunohistochemistry, Immunocytochemistry -
Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis
Authors: Mick J.M. van Eijs, José J.M. Ter Linde, Matthijs J.D. Baars, Mojtaba Amini, Miangela M. Laclé, Eelco C. Brand et al.
iScience
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Insight into immune profile associated with vitiligo onset and anti-tumoral response in melanoma patients receiving anti-PD-1 immunotherapy
Authors: Maria Luigia Carbone, Alessia Capone, Marika Guercio, Sofia Reddel, Domenico Alessandro Silvestris, Daniela Lulli et al.
Frontiers in Immunology
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Multilevel human secondary lymphoid immune system compartmentalization revealed by complementary imaging approaches
Authors: Oyler BL, Valencia-Dávila JA, Moysi E et al.
iScience
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IL17A mRNA Staining Distinguishes Palmoplantar Psoriasis from Hyperkeratotic Palmoplantar Eczema in Diagnostic Skin Biopsies
Authors: Jennifer S. Chen, Michael J. Murphy, Katelyn Singh, Alice Wang, Ryan D. Chow, Sa Rang Kim et al.
JID Innovations
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Clinical significance of CD155 expression and correlation with cellular components of tumor microenvironment in gastric adenocarcinoma
Authors: Xue Liu, Chenyang Xu, Tianwei Guo, Shenghua Zhan, Qiuying Quan, Mengsi Li et al.
Frontiers in Immunology
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Canakinumab leads to rapid reduction of neutrophilic inflammation and long-lasting response in Schnitzler syndrome
Authors: Simon Bossart, S. Morteza Seyed Jafari, Kristine Heidemeyer, Kexiang Yan, Laurence Feldmeyer, Luca Borradori et al.
Frontiers in Medicine
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Spatial transcriptomics landscape of lesions from non-communicable inflammatory skin diseases
Authors: A Schäbitz, C Hillig, M Mubarak, M Jargosch, A Farnoud, E Scala, N Kurzen, AC Pilz, N Bhalla, J Thomas, M Stahle, T Biedermann, CB Schmidt-We, F Theis, N Garzorz-St, K Eyerich, MP Menden, S Eyerich
Nature Communications, 2022-12-13;13(1):7729.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
The immune cell atlas of human neuroblastoma
Authors: Bronte Manouk Verhoeven, Shenglin Mei, Thale Kristin Olsen, Karin Gustafsson, Anders Valind, Axel Lindström et al.
Cell Reports Medicine
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MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data
Authors: Daniëlle Krijgsman, Neeraj Sinha, Matthijs J.D. Baars, Stephanie van Dam, Mojtaba Amini, Yvonne Vercoulen
STAR Protocols
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Single cell transcriptomic landscape of diabetic foot ulcers
Authors: G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin
Nature Communications, 2022-01-10;13(1):181.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Reduced Interleukin-17-Expressing Cells in Cutaneous Melanoma
Authors: A Tosi, L Nardinocch, ML Carbone, L Capriotti, E Pagani, S Mastroeni, C Fortes, F Scopelliti, C Cattani, F Passarelli, A Rosato, S D'Atri, CM Failla, A Cavani
Biomedicines, 2021-12-16;9(12):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Condomless receptive anal intercourse is associated with markers of mucosal inflammation in a cohort of men who have sex with men in Atlanta, Georgia
Authors: CF Kelley, I Pollack, R Yacoub, Z Zhu, VE Van Doren, S Gumber, RR Amara, V Fedirko, CS Kraft, TJB de Man, YJ Hu, C Grimsley A, PS Sullivan, RM Bostick
Journal of the International AIDS Society, 2021-12-01;24(12):e25859.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
IFN‐ gamma facilitates liver fibrogenesis by CD161+CD4+ T cells through a regenerative IL‐23/IL‐17 axis in chronic hepatitis B virus infection
Authors: Jing Li, Lisha Cheng, Haoyu Jia, Chun Liu, Siqi Wang, Yun Liu et al.
Clinical & Translational Immunology
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Chronic Hepatitis C Pathogenesis: Immune Response in the Liver Microenvironment and Peripheral Compartment
Authors: Daniela Alejandra Rios, Paola Cecilia Casciato, María Soledad Caldirola, María Isabel Gaillard, Cecilia Giadans, Beatriz Ameigeiras et al.
Frontiers in Cellular and Infection Microbiology
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ROR&gammat inhibitors block both IL-17 and IL-22 conferring a potential advantage over anti-IL-17 alone to treat severe asthma
Authors: D Lamb, D De Sousa, K Quast, K Fundel-Cle, JS Erjefält, C Sandén, HJ Hoffmann, M Kästle, R Schmid, K Menden, D Delic
Respiratory Research, 2021-05-22;22(1):158.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Hepatic lymphocytes involved in the pathogenesis of pediatric and adult non-alcoholic fatty liver disease
Authors: V Cairoli, E De Matteo, D Rios, C Lezama, M Galoppo, P Casciato, E Mullen, C Giadans, G Bertot, MV Preciado, P Valva
Scientific Reports, 2021-03-04;11(1):5129.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Interleukin-17A derived from mast cells contributes to fibrosis in gastric cancer with peritoneal dissemination
Authors: Katsuya Gunjigake, Jun Kinoshita, Takahisa Yamaguchi, Hiroto Saito, Daisuke Fujimori, Toshihide Horiike et al.
Gastric Cancer
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CD4 T cell-intrinsic role for the T helper 17 signature cytokine IL-17: Effector resistance to immune suppression
Authors: Michael P. Crawford, Sushmita Sinha, Pranav S. Renavikar, Nicholas Borcherding, Nitin J. Karandikar
Proceedings of the National Academy of Sciences
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Safe and Successful Treatment of Acute Cellular Rejection of an Intestine and Abdominal Wall Transplant With Vedolizumab
Authors: Guido Trentadue, Gursah Kats-Ugurlu, Tjasso Blokzijl, Gilles FH Diercks, Jan Willem Haveman, Klaas Nico Faber et al.
Transplantation Direct
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Ex vivo culture of lesional psoriasis skin for pharmacological testing
Authors: ML Tiirikaine, A Woetmann, H Norsgaard, LF Santamaria, P Lovato
J. Dermatol. Sci., 2019-12-27;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: Tissue Culture -
Paradoxical psoriasis induced by Tnf-alpha blockade shows immunological features typical of the early phase of psoriasis development
Authors: L Fania, M Morelli, C Scarponi, L Mercurio, F Scopelliti, C Cattani, GL Scaglione, T Tonanzi, MA Pilla, G Pagnanelli, C Mazzanti, G Girolomoni, A Cavani, S Madonna, C Albanesi
J Pathol Clin Res, 2019-10-29;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Interleukin (IL)-13 and IL-17A contribute to neo-osteogenesis in chronic rhinosinusitis by inducing RUNX2
Authors: R Khalmurato, HW Shin, DW Kim, JW Park
EBioMedicine, 2019-07-19;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Interleukin-17 Promotes Migration and Invasion of Human Cancer Cells Through Upregulation of MTA1 Expression
Authors: Na Guo, Ge Shen, Ying Zhang, Ahmed A. Moustafa, Dongxia Ge, Zongbing You
Frontiers in Oncology
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A systematic review and critical evaluation of inflammatory cytokine associations in hidradenitis suppurativa
Authors: John W. Frew, Jason E. Hawkes, James G. Krueger
F1000Research
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Composition and Clinical Impact of the Immunologic Tumor Microenvironment in Oral Squamous Cell Carcinoma
Authors: M Boxberg, L Leising, K Steiger, M Jesinghaus, A Alkhamas, M Mielke, N Pfarr, C Götz, KD Wolff, W Weichert, A Kolk
J. Immunol., 2018-12-10;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Lutzomyia longipalpis Saliva Drives Interleukin-17-Induced Neutrophil Recruitment Favoring Leishmania infantum Infection
Authors: Clarissa R. Teixeira, Claire da S. Santos, Deboraci B. Prates, Rafael T. dos Santos, Théo Araújo-Santos, Sebastião M. de Souza-Neto et al.
Frontiers in Microbiology
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A Reappraisal on the Potential Ability of Human Neutrophils to Express and Produce IL-17 Family Members In Vitro: Failure to Reproducibly Detect It
Authors: Nicola Tamassia, Fabio Arruda-Silva, Federica Calzetti, Silvia Lonardi, Sara Gasperini, Elisa Gardiman et al.
Frontiers in Immunology
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Increased interleukin-17A levels promote rituximab resistance by suppressing p53 expression and predict an unfavorable prognosis in patients with diffuse large B cell lymphoma
Authors: Weijie Zhong, Xin Xu, Zhigang Zhu, Li Yang, Hong Du, Zhongjun Xia et al.
International Journal of Oncology
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Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis
Authors: DA Rios, P Valva, PC Casciato, S Frias, M Soledad Ca, MI Gaillard, L Bezrodnik, J Bandi, O Galdame, B Ameigeiras, D Krasniansk, C Brodersen, E Mullen, EN Matteo, MV Preciado
Sci Rep, 2017-10-16;7(1):13283.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Zoonotic intestinal helminths interact with the canine immune system by modulating T cell responses and preventing dendritic cell maturation
Authors: J Junginger, K Raue, K Wolf, E Janecek, VM Stein, A Tipold, AR Günzel-Ape, C Strube, M Hewicker-T
Sci Rep, 2017-09-04;7(1):10310.
Species: Canine
Sample Types: Whole Cells
Applications: Flow Cytometry -
Expression of IL-23/Th17-related cytokines in basal cell carcinoma and in the response to medical treatments
Authors: C Pellegrini, A Orlandi, G Costanza, A Di Stefani, A Piccioni, A Di Cesare, A Chiricozzi, A Ferlosio, K Peris, MC Fargnoli
PLoS ONE, 2017-08-22;12(8):e0183415.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Idiopathic subglottic stenosis is associated with activation of the inflammatory IL-17A/IL-23 axis
Authors: Alexander Gelbard, Nicolas-George Katsantonis, Masanobu Mizuta, Dawn Newcomb, Joseph Rotsinger, Bernard Rousseau et al.
The Laryngoscope
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Characterization of IL-17AA and IL-17FF in rheumatoid arthritis and multiple sclerosis
Authors: Cheryl Schofield, Saloumeh K Fischer, Michael J Townsend, Sofia Mosesova, Kun Peng, Alvernia Francesca Setiadi et al.
Bioanalysis
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Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells
Authors: CT Seebauer, S Brunner, G Glockzin, P Piso, P Ruemmele, HJ Schlitt, EK Geissler, S Fichtner-F, R Kesselring
Oncoimmunology, 2016-10-14;5(12):e1242543.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Atopy and Inhaled Corticosteroid Use Associate with Fewer IL-17+ Cells in Asthmatic Airways
PLoS ONE, 2016-08-23;11(8):e0161433.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Impact of early cART in the gut during acute HIV infection
Authors: Claire Deleage, Alexandra Schuetz, W. Gregory Alvord, Leslie Johnston, Xing-Pei Hao, David R. Morcock et al.
JCI Insight
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Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues
Lab Invest, 2016-06-27;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Cardiac myosin-Th17 responses promote heart failure in human myocarditis
Authors: Jennifer M. Myers, Leslie T. Cooper, David C. Kem, Stavros Stavrakis, Stanley D. Kosanke, Ethan M. Shevach et al.
JCI Insight
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IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy
Authors: Neal L Millar
Sci Rep, 2016-06-06;6(0):27149.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
MRSA Infections in HIV-Infected People Are Associated with Decreased MRSA-Specific Th1 Immunity
Authors: Netanya S. Utay, Annelys Roque, J. Katherina Timmer, David R. Morcock, Claire DeLeage, Anoma Somasunderam et al.
PLOS Pathogens
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Development of Type 2, But Not Type 1, Leprosy Reactions is Associated with a Severe Reduction of Circulating and In situ Regulatory T-Cells
Authors: Ana Paula Vieira, Maria Ângela Bianconcini Avancini, Carla Pagliari, João Avancini, Alberto José da Silva Sakai-Valente, Alberto José da Silva Sakai-Valente et al.
The American Journal of Tropical Medicine and Hygiene
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A beneficial tumor microenvironment in oropharyngeal squamous cell carcinoma is characterized by a high T cell and low IL-17(+) cell frequency
Authors: Simone Punt, Emilie A. C. Dronkers, Marij J. P. Welters, Renske Goedemans, Senada Koljenović, Elisabeth Bloemena et al.
Cancer Immunology, Immunotherapy
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Association of Choroidal Interleukin-17-Producing T Lymphocytes and Macrophages with Geographic Atrophy
Authors: Serge Camelo, Sophie Lavelette, Xavier Guillonneau, William Raoul, Florian Sennlaub
Ophthalmologica
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Neuroendocrine tumors of the pancreas: a retrospective single-center analysis using the ENETS TNM-classification and immunohistochemical markers for risk stratification
Authors: Stefan M Brunner, Florian Weber, Jens M Werner, Ayman Agha, Stefan A Farkas, Hans J Schlitt et al.
BMC Surgery
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Selective inhibition of the p38 alternative activation pathway in infiltrating T cells inhibits pancreatic cancer progression
Authors: Muhammad S. Alam, Matthias M. Gaida, Frank Bergmann, Felix Lasitschka, Thomas Giese, Nathalia A. Giese et al.
Nature Medicine
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Association of Lung Inflammatory Cells with Small Airways Function and Exhaled Breath Markers in Smokers - Is There a Specific Role for Mast Cells?
Authors: Nussbaumer-Ochsner Y, Stolk J, Ferraz da Silva L, van Schadewijk A, de Jeu R, Prins F, Mauad T, Rabe K, Hiemstra P
PLoS ONE, 2015-06-12;10(6):e0129426.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
FoxP3(+) and IL-17(+) cells are correlated with improved prognosis in cervical adenocarcinoma
Authors: Simone Punt, Marjolein E. van Vliet, Vivian M. Spaans, Cornelis D. de Kroon, Gert Jan Fleuren, Arko Gorter et al.
Cancer Immunology, Immunotherapy
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RORgammat(+) hematopoietic cells are necessary for tumor cell proliferation during colitis-associated tumorigenesis in mice.
Authors: Martin M, Kesselring R, Saidou B, Brunner S, Schiechl G, Mouris V, Wege A, Rummele P, Schlitt H, Geissler E, Fichtner-Feigl S
Eur J Immunol, 2015-05-15;45(6):1667-79.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Correlations between immune response and vascularization qRT-PCR gene expression clusters in squamous cervical cancer.
Authors: Punt S, Houwing-Duistermaat J, Schulkens I, Thijssen V, Osse E, de Kroon C, Griffioen A, Fleuren G, Gorter A, Jordanova E
Mol Cancer, 2015-03-31;14(0):71.
Species: Human
Sample Types: Whole Cells
Applications: IHC-P -
Preferential recruitment of Th17 cells to cervical cancer via CCR6-CCL20 pathway.
Authors: Yu, Qing, Lou, Xiang-mi, He, Yan
PLoS ONE, 2015-03-13;10(3):e0120855.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Upregulation of IL-17A/F from human lung tissue explants with cigarette smoke exposure: implications for COPD.
Authors: Chang Y, Al-Alwan L, Alshakfa S, Audusseau S, Mogas A, Chouiali F, Nair P, Baglole C, Hamid Q, Eidelman D
Respir Res, 2014-11-27;15(1):145.
Species: Human
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot -
Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients.
Authors: Liu, Xiaosun, Jin, Hailong, Zhang, Geer, Lin, Xianke, Chen, Chao, Sun, Jianyi, Zhang, Yu, Zhang, Qing, Yu, Jiren
PLoS ONE, 2014-09-08;9(9):e106834.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
IL-17/Th17 pathway is activated in acne lesions.
Authors: Kelhala H, Palatsi R, Fyhrquist N, Lehtimaki S, Vayrynen J, Kallioinen M, Kubin M, Greco D, Tasanen K, Alenius H, Bertino B, Carlavan I, Mehul B, Deret S, Reiniche P, Martel P, Marty C, Blume-Peytavi U, Voegel J, Lauerma A
PLoS ONE, 2014-08-25;9(8):e105238.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Allergic contact dermatitis in psoriasis patients: typical, delayed, and non-interacting.
Authors: Quaranta M, Eyerich S, Knapp B, Nasorri F, Scarponi C, Mattii M, Garzorz N, Harlfinger A, Jaeger T, Grosber M, Pennino D, Mempel M, Schnopp C, Theis F, Albanesi C, Cavani A, Schmidt-Weber C, Ring J, Eyerich K
PLoS ONE, 2014-07-24;9(7):e101814.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Interleukin-17A promotes MUC5AC expression and goblet cell hyperplasia in nasal polyps via the Act1-mediated pathway.
Authors: Xia, Wentong, Bai, Jing, Wu, Xingmei, Wei, Yi, Feng, Shaoyan, Li, Lei, Zhang, Jia, Xiong, Guanxia, Fan, Yunping, Shi, Jianbo, Li, Huabin
PLoS ONE, 2014-06-03;9(6):e98915.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Sera from remitting and secondary progressive multiple sclerosis patients disrupt the blood-brain barrier.
Authors: Shimizu F, Tasaki A, Sano Y, Ju M, Nishihara H, Oishi M, Koga M, Kawai M, Kanda T
PLoS ONE, 2014-03-31;9(3):e92872.
Species: Human
Sample Types: Serum
Applications: Neutralization -
Th1 response and systemic treg deficiency in inclusion body myositis.
Authors: Allenbach Y, Chaara W, Rosenzwajg M, Six A, Prevel N, Mingozzi F, Wanschitz J, Musset L, Charuel J, Eymard B, Salomon B, Duyckaerts C, Maisonobe T, Dubourg O, Herson S, Klatzmann D, Benveniste O
PLoS ONE, 2014-03-04;9(3):e88788.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
The effect of adalimumab on key drivers in the pathogenesis of psoriasis.
Authors: Hendriks A, van der Velden H, Wolberink E, Seyger M, Schalkwijk J, Zeeuwen P, de Jong E, Pasch M, van Erp P, van de Kerkhof P
Br J Dermatol, 2014-03-01;170(3):571-80.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
IL-17A induces endothelial inflammation in systemic sclerosis via the ERK signaling pathway.
Authors: Xing X, Yang J, Yang X, Wei Y, Zhu L, Gao D, Li M
PLoS ONE, 2013-12-23;8(12):e85032.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: IHC-P, Neutralization -
Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A.
Authors: Lotti, Fiorenza, Jarrar, Awad M, Pai, Rish K, Hitomi, Masahiro, Lathia, Justin, Mace, Adam, Gantt, Gerald A, Sukhdeo, Kumar, DeVecchio, Jennifer, Vasanji, Amit, Leahy, Patrick, Hjelmeland, Anita B, Kalady, Matthew, Rich, Jeremy N
J Exp Med, 2013-12-09;210(13):2851-72.
Species: Human
Sample Types: Whole Cells
Applications: IHC, Neutralization -
In vivo induction of cutaneous inflammation results in the accumulation of extracellular trap-forming neutrophils expressing RORgammat and IL-17.
Authors: Keijsers R, Hendriks A, van Erp P, van Cranenbroek B, van de Kerkhof P, Koenen H, Joosten I
J Invest Dermatol, 2013-12-06;134(5):1276-84.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
Cytokine, 2013-10-12;64(3):660-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
The Transcription Factor CREB Enhances Interleukin-17A Production and Inflammation in a Mouse Model of Atherosclerosis
Authors: Sivareddy Kotla, Nikhlesh K. Singh, Mark R. Heckle, Gabor J. Tigyi, Gadiparthi N. Rao
Science Signaling
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An interleukin-17–mediated paracrine network promotes tumor resistance to anti-angiogenic therapy
Authors: Alicia S Chung, Xiumin Wu, Guanglei Zhuang, Hai Ngu, Ian Kasman, Jianhuan Zhang et al.
Nature Medicine
-
High IL-17-positive tumor immune cell infiltration is indicative for chemosensitivity of ovarian carcinoma
Authors: Raoul A. Droeser, Uwe Güth, Serenella Eppenberger-Castori, Sylvia Stadlmann, Christian Hirt, Luigi Terracciano et al.
Journal of Cancer Research and Clinical Oncology
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Chemokine (C-C Motif) Ligand 20, a Potential Biomarker for Graves' Disease, Is Regulated by Osteopontin
Authors: Xiaoli Li, Yicheng Qi, Xinran Ma, Fengjiao Huang, Hua Guo, Xiaohua Jiang et al.
PLoS ONE
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Interleukin-17A+ cell counts are increased in systemic sclerosis skin and their number is inversely correlated with the extent of skin involvement.
Authors: Truchetet M, Brembilla N, Montanari E, Lonati P, Raschi E, Zeni S, Fontao L, Meroni P, Chizzolini C
Arthritis Rheum, 2013-05-01;65(5):1347-56.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke.
Authors: Gelderblom M, Weymar A, Bernreuther C, Velden J, Arunachalam P, Steinbach K, Orthey E, Arumugam T, Leypoldt F, Simova O, Thom V, Friese M, Prinz I, Holscher C, Glatzel M, Korn T, Gerloff C, Tolosa E, Magnus T
Blood, 2012-09-13;120(18):3793-802.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Human CCR4+ CCR6+ Th17 cells suppress autologous CD8+ T cell responses
Authors: Fei Zhao, Bastian Hoechst, Jaba Gamrekelashvili, Lars A. Ormandy, Torsten Voigtländer, Heiner Wedemeyer et al.
The Journal of Immunology
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Differential pattern and prognostic significance of CD4+, FOXP3+ and IL-17+ tumor infiltrating lymphocytes in ductal and lobular breast cancers.
Authors: Droeser R, Zlobec I, Kilic E, Guth U, Heberer M, Spagnoli G, Oertli D, Tapia C
BMC Cancer, 2012-04-03;12(0):134.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Interleukin-17 expression in neutrophils and Th17 cells in cutaneous T-cell lymphoma associated with neutrophilic infiltrate of the skin.
Authors: Fontao L, Brembilla NC, Masouye I, Kaya G, Prins C, Dupin N, Saurat JH, Chizzolini C, Piguet V
Br. J. Dermatol., 2012-01-09;166(3):687-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
RASA1 maintains the lymphatic vasculature in a quiescent functional state in mice.
Authors: Lapinski PE, Kwon S, Lubeck BA
J. Clin. Invest., 2012-01-09;122(2):733-47.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: IHC-P, Neutralization -
Induction of endothelial nitric oxide synthase expression by IL-17 in human vascular endothelial cells: implications for vascular remodeling in transplant vasculopathy.
Authors: Liu AC, Lee M, McManus BM, Choy JC
J. Immunol., 2012-01-04;188(3):1544-50.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: IHC, Neutralization -
MIP-3 alpha Expression in Macrophages Is NOD Dependent
Authors: M. Hausmann, C. Zeitler, A. Weber, M. Krebs, S. Kellermeier, P. Rosenstiel et al.
Digestion
-
CD8 positive T cells express IL-17 in patients with chronic obstructive pulmonary disease
Authors: Ying Chang, Jessica Nadigel, Nicholas Boulais, Jean Bourbeau, François Maltais, David H Eidelman et al.
Respiratory Research
-
IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
Clin. Cancer Res., 2011-11-02;17(24):7529-38.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
The Th17 Pathway in Cystic Fibrosis Lung Disease
Authors: Hui-Leng Tan, Nicolas Regamey, Sarah Brown, Andrew Bush, Clare M. Lloyd, Jane C. Davies
American Journal of Respiratory and Critical Care Medicine
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Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
Authors: Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C
Br. J. Dermatol., 2011-05-17;164(6):1228-34.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Delineation of a cellular hierarchy in lung cancer reveals an oncofetal antigen expressed on tumor-initiating cells.
Authors: Damelin M, Geles KG, Follettie MT, Yuan P, Baxter M, Golas J, Dijoseph JF, Karnoub M, Huang S, Diesl V, Behrens C, Choe SE, Rios C, Gruzas J, Sridharan L, Dougher M, Kunz A, Hamann PR, Evans D, Armellino D, Khandke K, Marquette K, Tchistiakova L, Boghaert ER, Abraham RT, Wistuba II, Zhou BB
Cancer Res., 2011-05-03;71(12):4236-46.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Overrepresentation of IL-17A and IL-22 producing CD8 T cells in lesional skin suggests their involvement in the pathogenesis of psoriasis.
Authors: Res PC, Piskin G, de Boer OJ, van der Loos CM, Teeling P, Bos JD, Teunissen MB
PLoS ONE, 2010-11-24;5(11):e14108.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD.
Authors: Doe C, Bafadhel M, Siddiqui S, Desai D, Mistry V, Rugman P, McCormick M, Woods J, May R, Sleeman MA, Anderson IK, Brightling CE
Chest, 2010-06-10;138(5):1140-7.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
J. Immunol., 2010-03-31;184(9):4880-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Engineering of stable bispecific antibodies targeting IL-17A and IL-23
Authors: Robert Mabry, Katherine E. Lewis, Margaret Moore, Patricia A. McKernan, Thomas R. Bukowski, Kristen Bontadelli et al.
Protein Engineering, Design and Selection
-
IL-17 induces monocyte migration in rheumatoid arthritis.
Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
J. Immunol., 2009-03-15;182(6):3884-91.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
J. Allergy Clin. Immunol., 2008-12-03;123(1):59-66.e4.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Increased numbers of circulating polyfunctional Th17 memory cells in patients with seronegative spondylarthritides.
Authors: Jandus C, Bioley G, Rivals JP, Dudler J, Speiser D, Romero P
Arthritis Rheum., 2008-08-01;58(8):2307-17.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-P -
Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers.
Authors: Nistala K, Moncrieffe H, Newton KR, Varsani H, Hunter P, Wedderburn LR
Arthritis Rheum., 2008-03-01;58(3):875-87.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum.
Authors: Witowski J, Ksiazek K, Warnecke C, Kuzlan M, Korybalska K, Tayama H, Wisniewska-Elnur J, Pawlaczyk K, Trominska J, Breborowicz A, Jorres A
Kidney Int., 2007-01-17;71(6):514-25.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
J. Immunol., 2005-11-01;175(9):6177-89.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
J. Immunol., 2005-02-15;174(4):2376-86.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
Authors: Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P
Am. J. Pathol., 2004-02-01;164(2):409-17.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Interleukin-17 induces angiogenesis in�vitro via CXCL8 and CCL2 in retinal pigment epithelium
Authors: Y Chen, M Zhong, G Yuan, H Peng
Mol Med Rep, 2018-01-18;0(0):.
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