Human IL-13 PE-conjugated Antibody Summary
Gly35-Asn146
Accession # P35225
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑13 in NS0 Mouse Cell Line Transfected with Human IL-13 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human IL-13 was stained with Mouse Anti-Human IL-13 PE-conjugated Monoclonal Antibody (Catalog # IC2131P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Background: IL-13
IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature human IL-13 shares 57%, 59%, and 94% amino acid sequence identity with mouse, rat, and rhesus macaque IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of pro-inflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is over-expressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).
- Wills-Karp, M. (2004) Immunol. Rev. 202:175.
- Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
- McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3735.
- Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
- Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
- Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
- Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
- Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
- Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
- Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
- Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
- Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
- Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
- Tabata, Y. et al. (2007) J. Immunol. 177:7905.
- Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
- Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
- Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.
Product Datasheets
Citations for Human IL-13 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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A novel population of human CD56+ human leucocyte antigen D-related (HLA-DR+) colonic lamina propria cells is associated with inflammation in ulcerative colitis.
Authors: Ng SC, Plamondon S, Al-Hassi HO, English N, Gellatly N, Kamm MA, Knight SC, Stagg AJ
Clin. Exp. Immunol., 2009-08-12;158(2):205-18.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
T-cell activation during exacerbations: a longitudinal study in refractory asthma.
Authors: Mamessier E, Nieves A, Lorec AM, Dupuy P, Pinot D, Pinet C, Vervloet D, Magnan A
Allergy, 2008-09-01;63(9):1202-10.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
J. Immunol., 2008-04-01;180(7):5109-17.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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