Human IkB-epsilon Antibody

Catalog #: AF4300 Datasheet

Catalog #	Availability	Size / Price	Qty
AF4300
AF4300-SP

1 Image

All IkB-epsilon Antibodies

Product Details

FAQs

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IkB-epsilon Antibody Summary

Species Reactivity

Human

Specificity

Detects human IkB-epsilon in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse IkB-epsilon is observed and less than 1% cross-reactivity with recombinant human IkB-beta is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IkB-epsilon
Ser141-Asp500
Accession # O00221

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

IkB‑ epsilon in Human Astrocytoma. IkB-e was detected in immersion fixed paraffin-embedded sections of human astrocytoma using Goat Anti-Human IkB-e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4300) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IkB-epsilon

NF kappa B inhibitor epsilon (IkB-epsilon ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-epsilon degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.

Long Name

I-kappa-B-epsilon

Entrez Gene IDs

4794 (Human); 18037 (Mouse)

Alternate Names

epsilon; ikappaBepsilon; I-kappa-B-epsilon; IkB-E; IKBENF-kappa-B inhibitor epsilon; IkBepsilon; IkB-epsilon; NF-kappa-BIE; NFKBIE; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor