Human IkB-epsilon Antibody

Catalog #: MAB4300 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB4300
MAB4300-SP

Detection of Human IkB-epsilon by Western Blot.

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All IkB-epsilon Antibodies

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Human IkB-epsilon Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human and mouse IkB-epsilon in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 418515

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IkB-epsilon
Ser64-Asp423
Accession # Q96F31

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IkB-epsilon by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line, PC-3 human prostate cancer cell line, Raji human Burkitt's lymphoma cell line, LNCaP human prostate cancer cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human IkB-e Monoclonal Antibody (Catalog # MAB4300) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IkB-e at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IkB-epsilon

NF kappa B inhibitor epsilon (IkB epsilon ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB epsilon degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.

Long Name

I-kappa-B-epsilon

Entrez Gene IDs

4794 (Human); 18037 (Mouse)

Alternate Names

epsilon; ikappaBepsilon; I-kappa-B-epsilon; IkB-E; IKBENF-kappa-B inhibitor epsilon; IkBepsilon; IkB-epsilon; NF-kappa-BIE; NFKBIE; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor