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Human IGFBP-3 Antibody

Catalog #: MAB305
13 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB305-SP
MAB305-500
MAB305-100
IGFBP‑3 in Human Placenta.
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Product Details
Citations (13)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGFBP-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGFBP-3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.25% cross-reactivity with recombinant human (rh) IGF‑I R, rhIGFBP-1, rhIGFBP-2, rhIGFBP-4, rhIGFBP-5, and rhIGFBP-6 is observed.
Source
Monoclonal Mouse IgG2B Clone # 84728
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGFBP‑3
Gly28-Lys291
Accession # CAA46087
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IGFBP‑3 (Catalog # 675-B3)
Immunohistochemistry
8-25 µg/mL
See below

Human IGFBP-3 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IGFBP‑3 Biotinylated Antibody (Catalog # BAF675)

Standard: Recombinant Human IGFBP-3 Protein, CF (Catalog # 675-B3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry IGFBP-3 antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

IGFBP‑3 in Human Placenta. IGFBP-3 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human IGFBP-3 Monoclonal Antibody (Catalog # MAB305) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Mouse IGFBP-3 by Western Blot View Larger

Detection of Mouse IGFBP-3 by Western Blot The effect of tumour carriage on skeletal muscle Skeletal-muscle samples were obtained from normal athymic mice (group N, n = 14) and from those that carried MiaPaCa2 (group M, n = 10) or AsPC1 cells (group A, n = 13). Western blots were performed to determine atrogin-1 (a), IGFBP-3 (a), myosin (b), and MURF1 (b), using beta -tubulin as a loading control. The blots are representative data. The histograms show the results of all mice (See n in the parentheses). *P < 0.05, NS: not significant Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4271-3), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Human IGFBP-3 Antibody by Western Blot View Larger

Detection of Mouse Human IGFBP-3 Antibody by Western Blot The effect of tumour carriage on skeletal muscle Skeletal-muscle samples were obtained from normal athymic mice (group N, n = 14) and from those that carried MiaPaCa2 (group M, n = 10) or AsPC1 cells (group A, n = 13). Western blots were performed to determine atrogin-1 (a), IGFBP-3 (a), myosin (b), and MURF1 (b), using beta -tubulin as a loading control. The blots are representative data. The histograms show the results of all mice (See n in the parentheses). *P < 0.05, NS: not significant Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29609556), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-3

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Human IGFBP-3 cDNA encodes a 291 amino acid (aa) residue precursor protein with a putative 27 aa residue signal peptide that is processed to generate the 264 aa residue mature protein with three potential N-linked and two potential O-linked glycosylation sites. Human IGFBP-3 is expressed in multiple tissues. The highest expression level is found in the non-paranchymal cells of the liver. Expression levels are also higher during extrauterine life and peak during puberty. Human IGFBP-3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGF-I or IGF-II and the acid-labile subunit (ALS).

References
  1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
  2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
  3. Spagnoli, A. and R.G. Rosenfeld (1997) Curr. Op. Endocrinology and Diabetes 4:1.
Long Name
Insulin-like Growth Factor Binding Protein 3
Entrez Gene IDs
3486 (Human); 16009 (Mouse)
Alternate Names
acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; IBP-3; IBP3BP-53; IGF-binding protein 3; IGFBP3; IGFBP-3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3

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Citations for Human IGFBP-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Putative tumor metastasis-associated genes in human gastric cancer
    Authors: TSUNG-FAN TUAN, CHENG-TA CHUNG, HSIAO-HUI TSOU, FONG-WEN CHEN, HENG-LIANG LIN, YIU-KAY LAI et al.
    International Journal of Oncology
  2. Expanded CUG Repeat RNA Induces Premature Senescence in Myotonic Dystrophy Model Cells
    Authors: Hasuike Y, Mochizuki H, Nakamori M.
    Frontiers in Genetics
  3. Expanded CUG Repeat RNA Induces Premature Senescence in Myotonic Dystrophy Model Cells
    Authors: Hasuike Y, Mochizuki H, Nakamori M.
    Frontiers in Genetics
  4. Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer
    Authors: HJ Shih, HF Chang, CL Chen, PL Torng
    Scientific Reports, 2021-11-25;11(1):22925.
    Species: Human, Xenograft
    Sample Types: Recombinant Proteins, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Neutralization
  5. Enhanced Expression of IGFBP-3 Reduces Radiosensitivity and Is Associated with Poor Prognosis in Oral Squamous Cell Carcinoma
    Authors: J Sakata, A Hirosue, R Yoshida, Y Matsuoka, K Kawahara, H Arita, H Nakashima, T Yamamoto, M Nagata, S Kawaguchi, S Gohara, Y Nagao, K Yamana, R Toya, R Murakami, Y Kuwahara, M Fukumoto, H Nakayama
    Cancers (Basel), 2020-02-20;12(2):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. The participation of insulin-like growth factor-binding protein 3 released by astrocytes in the pathology of Alzheimer's disease.
    Authors: Watanabe K, Uemura K, Asada M, Maesako M, Akiyama H, Shimohama S, Takahashi R, Kinoshita A
    Mol Brain, 2015-12-04;8(1):82.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. IGFBP3 promotes esophageal cancer growth by suppressing oxidative stress in hypoxic tumor microenvironment.
    Authors: Natsuizaka M, Kinugasa H, Kagawa S, Whelan K, Naganuma S, Subramanian H, Chang S, Nakagawa K, Rustgi N, Kita Y, Natsugoe S, Basu D, Gimotty P, Klein-Szanto A, Diehl J, Nakagawa H
    Am J Cancer Res, 2014-01-15;4(1):29-41.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. Plasminogen activator inhibitor 1--insulin-like growth factor binding protein 3 cascade regulates stress-induced senescence.
    Authors: Elzi, David J, Lai, Yanlai, Song, Meihua, Hakala, Kevin, Weintraub, Susan T, Shiio, Yuzuru
    Proc Natl Acad Sci U S A, 2012-07-09;109(30):12052-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Human IGF Binding Protein-3 Overexpression Impairs Glucose Regulation in Mice via an Inhibition of Insulin Secretion.
    Authors: Nguyen KH, Yao XH, Moulik S, Mishra S, Nyomba BL
    Endocrinology, 2011-03-29;152(6):2184-96.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  10. Epidermal growth factor receptor regulates aberrant expression of insulin-like growth factor-binding protein 3.
    Authors: Takaoka M, Harada H, Andl CD, Oyama K, Naomoto Y, Dempsey KL, Klein-Szanto AJ, El-Deiry WS, Grimberg A, Nakagawa H
    Cancer Res., 2004-11-01;64(21):7711-23.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  11. Mutual regulation between IGF‐1R and IGFBP‐3 in human corneal epithelial cells
    Authors: Rossella Titone, Meifang Zhu, Danielle M. Robertson
    Journal of Cellular Physiology
  12. IGFBP-3 Nuclear Localization Predicts Human Prostate Cancer Recurrence
    Authors: David B. Seligson, Hong Yu, Sheila Tze, Jonathan Said, Allan J. Pantuck, Pinchas Cohen et al.
    Hormones and Cancer
  13. Insulin-like growth factor-independent insulin-like growth factor binding protein 3 promotes cell migration and lymph node metastasis of oral squamous cell carcinoma cells by requirement of integrin beta 1
    Authors: Yi-Chen Yen, Jenn-Ren Hsiao, Shih Sheng Jiang, Jeffrey S. Chang, Ssu-Han Wang, Ying-Ying Shen et al.
    Oncotarget

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