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Human IGFBP-2 Antibody

Catalog #: AF674
12 Citations Datasheet
Catalog # Availability Size / Price Qty
AF674
AF674-SP
Detection of Human IGFBP‑2 by Western Blot.
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Product Details
Citations (12)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGFBP-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGFBP-2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGFBP‑2
Glu40-Gln328
Accession # CAA34373
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
25 µg/mL
See below
Neutralization
Measured by its ability to neutralize IGFBP‑2 inhibition of IGF‑II-dependent proliferation in the MCF‑7 human breast cancer cell line. The Neutralization Dose (ND50) is typically 2.5-7.5 µg/mL in the presence of 0.2 µg/mL Recombinant Human IGFBP‑2 and 14 ng/mL Recombinant Human IGF‑II.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IGFBP-2 antibody by Western Blot. View Larger

Detection of Human IGFBP‑2 by Western Blot. Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IGFBP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IGFBP-2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human IGFBP-2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human IGFBP‑2 by Simple WesternTM. Simple Western lane view shows lysates of T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for IGFBP-2 at approximately 42 kDa (as indicated) using 25 µg/mL of Goat Anti-Human IGFBP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Neutralization IGFBP‑2 Inhibition of IGF‑II-dependent Cell Proliferation and Neutralization by Human IGFBP‑2 Antibody. View Larger

IGFBP‑2 Inhibition of IGF‑II-dependent Cell Proliferation and Neutralization by Human IGFBP‑2 Antibody. Recombinant Human IGFBP-2 (Catalog # 674-B2) inhibits Recombinant Human IGF-II (Catalog # 292-G2) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF-II (14 ng/mL) activity elicited by Recombinant Human IGFBP-2 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGFBP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674). The ND50 is typically 2.5-7.5 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-2

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Human IGFBP-2 cDNA encodes a 328 amino acid (aa) residue precursor protein with a putative 39 aa residue signal peptide that is processed to generate the 289 aa residue mature protein. IGFBP-2 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues. IGFBP-2 binds preferentially to IGF-II, exhibiting a 2-10 fold higher affinity for IGF-II than for IGF-I.

References
  1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
  2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
Long Name
Insulin-like Growth Factor Binding Protein 2
Entrez Gene IDs
3485 (Human); 16008 (Mouse)
Alternate Names
BP2; IBP2; IBP-2; IGF-binding protein 2; IGFBP2; IGFBP-2; IGF-BP53; insulin-like growth factor binding protein 2 (36kD); insulin-like growth factor binding protein 2, 36kDa; insulin-like growth factor-binding protein 2

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Citations for Human IGFBP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma
    Authors: Tan X, Shi L, Banerjee P et al.
    J. Clin. Invest.
  2. IGFBP2 secretion by mammary adipocytes limits breast cancer invasion
    Authors: Conway JRW, Dinç DD, Follain G et al.
    Science advances
  3. Age-related increases in IGFBP2 increase melanoma cell invasion and lipid synthesis
    Authors: Alicea, GM;Portuallo, ME;Patel, P;Fane, ME;Carey, AE;Speicher, D;Tang, HY;Kossenkov, AV;Rebecca, VW;Wirtz, DG;Weeraratna, AT;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. IGFBP2 promotes proliferation and cell migration through STAT3 signaling in Sonic hedgehog medulloblastoma
    Authors: H Kunhiraman, L McSwain, SW Shahab, TR Gershon, TJ MacDonald, AM Kenney
    Acta neuropathologica communications, 2023-04-08;11(1):62.
    Species: Human
    Sample Types: Whole Cell
    Applications: Neutralization
  5. Secreted PGK1 and IGFBP2 contribute to the bystander effect of miR-10b gene editing in glioma
    Authors: Yanhong Zhang, Rosalia Rabinovsky, Zhiyun Wei, Rachid El Fatimy, Evgeny Deforzh, Bai Luan et al.
    Molecular Therapy - Nucleic Acids
  6. Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system
    Authors: A Weigert, X Zheng, A Nenzel, K Turkowski, S Günther, E Strack, E Sirait-Fis, E Elwakeel, IM Kur, VS Nikam, C Valasaraja, H Winter, A Wissgott, R Voswinkel, F Grimminger, B Brüne, W Seeger, SS Pullamsett, R Savai
    Nature Communications, 2022-10-14;13(1):6078.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Metallothionein 2A Expression in Cancer-Associated Fibroblasts and Cancer Cells Promotes Esophageal Squamous Cell Carcinoma Progression
    Authors: M Shimizu, YI Koma, H Sakamoto, S Tsukamoto, Y Kitamura, S Urakami, K Tanigawa, T Kodama, N Higashino, M Nishio, M Shigeoka, Y Kakeji, H Yokozaki
    Cancers, 2021-09-10;13(18):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Intracellular Insulin-like growth factor binding protein 2 (IGFBP2) contributes to the senescence of keratinocytes in psoriasis by stabilizing cytoplasmic p21
    Authors: L Mercurio, D Lulli, F Mascia, E Dellambra, C Scarponi, M Morelli, C Valente, ML Carbone, S Pallotta, G Girolomoni, C Albanesi, S Pastore, S Madonna
    Aging (Albany NY), 2020-04-17;12(8):6823-6851.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  9. Mechanisms utilized by feline adipose-derived mesenchymal stem cells to inhibit T lymphocyte proliferation
    Authors: N Taechangam, SS Iyer, NJ Walker, B Arzi, DL Borjesson
    Stem Cell Res Ther, 2019-06-25;10(1):188.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Glioma progression is mediated by an addiction to aberrant IGFBP2 expression and can be blocked using anti-IGFBP2 strategies
    Authors: Lynette M. Phillips, Xinhui Zhou, David E. Cogdell, Corrine Yingxuan Chua, Anouk Huisinga, Kenneth R. R Hess et al.
    The Journal of Pathology
  11. Insulin-like growth factor-binding protein 2 secreted by a tumorigenic cell line supports ex vivo expansion of mouse hematopoietic stem cells.
    Authors: Huynh H, Iizuka S, Kaba M, Kirak O, Zheng J, Lodish HF, Zhang CC
    Stem Cells, 2008-03-27;26(6):1628-35.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
    Authors: McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
    Gastroenterology, 2006-03-06;130(6):1754-63.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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