Human IGF-I R/IGF1R Fluorescein-conjugated Antibody

Catalog #: FAB391F
3 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
FAB391F-100
FAB391F-025

Detection of IGF‑I R in MCF‑7 Human Cell Line by Flow Cytometry.

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All IGF-I R/IGF1R Antibodies

Product Details

Citations (3)

FAQs

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGF-I R/IGF1R Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IGF-I R in sandwich immunoassays and Western blots. In sandwich immunoassays, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF-II, rhIL-3 R alpha, rhIL-9 R, and rhTGF-beta RII.

Source

Monoclonal Mouse IgG₁ Clone # 33255

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I R
Glu31-Asn932
Accession # P08069

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IGF‑I R in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human IGF-I R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB391F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IGF-I R/IGF1R

Insulin-like Growth Factor I Receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.

Long Name

Insulin-like Growth Factor I Receptor

Entrez Gene IDs

3480 (Human)

Alternate Names

CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human IGF-I R/IGF1R Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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IGFBP6 controls the expansion of chemoresistant glioblastoma through paracrine IGF2/IGF-1R signaling
Authors: Claudia R. Oliva, Brian Halloran, Anita B. Hjelmeland, Ana Vazquez, Shannon M. Bailey, Jann N. Sarkaria et al.
Cell Communication and Signaling
Insulin-like growth factor 1 receptor and response to anti-IGF1R antibody therapy in osteosarcoma.
Authors: Cao, Yu, Roth, Michael, Piperdi, Sajida, Montoya, Kristofe, Sowers, Rebecca, Rao, Pulivart, Geller, David, Houghton, Peter, Kolb, E Anders, Gill, Jonathan, Gorlick, Richard
PLoS ONE, 2014-08-29;9(8):e106249.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, Flow Cytometry Control
Growth hormone-induced stimulation of multilineage human hematopoiesis.
Authors: Hanley MB, Napolitano LA, McCune JM
Stem Cells, 2005-06-13;23(8):1170-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry