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Human IFN-gamma Fluorescein-conjugated Antibody

Catalog #: IC285F
9 Citations Datasheet
Catalog # Availability Size / Price Qty
IC285F-100
Detection of IFN‑ gamma in Human PBMCs by Flow Cytometry.
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Citations (9)
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Human IFN-gamma Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma, recombinant rat IFN-gamma, or recombinant porcine IFN-gamma is observed.
Source
Monoclonal Mouse IgG2B Clone # 25723
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IFN-gamma
Gln24-Gln166
Accession # AAP20098.1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IFN-? antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IFN‑ gamma in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA, 1 µg/mL Ionomycin, and 3 µM Monensin overnight were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human IFN-gamma Fluorescein-conjugated Monoclonal Antibody (Catalog # IC285F) or (B) Mouse IgG2BFluorescein Isotype Control (Catalog # IC0041F). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IFN-gamma

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

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Citations for Human IFN-gamma Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism
    Authors: Fernando, V;Zheng, X;Sharma, V;Furuta, S;
    bioRxiv : the preprint server for biology
  2. Trichinella spiralis Excretory-Secretory Products Induce Tolerogenic Properties in Human Dendritic CellsviaToll-Like Receptors 2 and 4
    Authors: N Ilic, A Gruden-Mov, J Cvetkovic, S Tomic, DB Vucevic, C Aranzamend, M Colic, E Pinelli, L Sofronic-M
    Front Immunol, 2018-01-24;9(0):11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
    Sci Rep, 2016-09-02;6(0):32320.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. B Lymphocytes in Multiple Sclerosis: Bregs and BTLA/CD272 Expressing-CD19+ Lymphocytes Modulate Disease Severity
    Sci Rep, 2016-07-14;6(0):29699.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Expansion of anti-mesothelin specific CD4+ and CD8+ T cell responses in patients with pancreatic carcinoma.
    Authors: Chen Y, Ayaru L, Mathew S, Morris E, Pereira S, Behboudi S
    PLoS ONE, 2014-02-10;9(2):e88133.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. IL-10-producing regulatory B cells in the pathogenesis of chronic hepatitis B virus infection.
    Authors: Das, Abhishek, Ellis, Gidon, Pallant, Celeste, Lopes, A Ross, Khanna, Pooja, Peppa, Dimitra, Chen, Antony, Blair, Paul, Dusheiko, Geoffrey, Gill, Upkar, Kennedy, Patrick, Brunetto, Maurizia, Lampertico, Pietro, Mauri, Claudia, Maini, Mala K
    J Immunol, 2012-09-12;189(8):3925-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. NF-kappaB modulates sensitivity to apoptosis, proinflammatory and migratory potential in short- versus long-term cultured human gammadelta lymphocytes.
    Authors: Ferrarini M, Delfanti F, Gianolini M, Rizzi C, Alfano M, Lazzarin A, Biswas P
    J. Immunol., 2008-11-01;181(9):5857-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Overlapping human leukocyte antigen class I/II binding peptide vaccine for the treatment of patients with stage IV melanoma: evidence of systemic immune dysfunction.
    Authors: Celis E, Celis E
    Cancer, 2007-07-01;110(1):203-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
    Authors: D'Ambrosio D
    Eur. J. Immunol., 2002-10-01;32(10):2934-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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