Human IFN-gamma ELISpot Development Module, 5 Plate Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
PRODUCT SUMMARY
Complete ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Kits are available for detection and enumeration of a single analyte or two analytes simultaneously. Complete ELISpot kits are ready-to-run and require no assay development or refinement. ELISpot Development Modules contain the basic components required to develop an ELISpot assay. They offer an economical alternative to buying separate antibodies.
ELISpot development modules are an alternative to ELISpot kits. A basic understanding of ELISpot assay development is required for the successful use of these reagents. Each investigator should optimize the coating conditions, the assay sensitivity, the type of enzyme and substrate, as well as the concentrations of the capture and detection antibodies to achieve desired results. The analyte-specific ELISpot Development Module and the ELISpot Blue Color Module contain the necessary components for analyte detection and visualization, respectively. These modules can be used together but are sold separately. Each module contains enough reagents for at least five 96-well microplates.
PRODUCT FEATURES
- An economical alternative to ELISpot Kits
- Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
- Generic development protocols provide direction to start an optimization protocol
- Customize the assay to your specific needs
MODULE CONTENTS
- Human IFN-gamma Capture Antibody
- Human IFN-gamma Biotinylated-conjugated Detection Antibody
OTHER REAGENTS REQUIRED
- ELISpot Blue Color Module or equivalent (R&D Systems, Catalog # SEL002)
- PBS - 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
- Wash Buffer - 0.05% Tween® 20 in PBS
- Blocking Buffer - 1% BSA, 5% Sucrose in PBS
- Reagent Diluent - 1% BSA in PBS, pH 7.2 - 7.4, 0.2 µm filtered
- 2 °C – 8 °C refrigerator
- 37 °C CO2 incubator
- Positive Control - Use Recombinant Human IFN-gamma or cells known to secrete Human IFN-gamma
- 96-well plates - Nitrocellulose-bottom plates, PVDF-bottom Immunospot® plates, or flat-bottom polystyrene Immulon® ELISA plates
- Multi-channel pipette, squirt bottle, manifold dispenser, or automated microplate washer
- Dissection microscope or an automated ELISpot Reader
- Deionized H2O
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Product Datasheets
Preparation and Storage
Background: IFN-gamma
IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
Citations for Human IFN-gamma ELISpot Development Module, 5 Plate
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Cranioencephalic functional lymphoid units in glioblastoma
Authors: Dobersalske, C;Rauschenbach, L;Hua, Y;Berliner, C;Steinbach, A;Grüneboom, A;Kokkaliaris, KD;Heiland, DH;Berger, P;Langer, S;Tan, CL;Stenzel, M;Landolsi, S;Weber, F;Darkwah Oppong, M;Werner, RA;Gull, H;Schröder, T;Linsenmann, T;Buck, AK;Gunzer, M;Stuschke, M;Keyvani, K;Forsting, M;Glas, M;Kipnis, J;Steindler, DA;Reinhardt, HC;Green, EW;Platten, M;Tasdogan, A;Herrmann, K;Rambow, F;Cima, I;Sure, U;Scheffler, B;
Nature medicine
Species: Human
Sample Types: Whole Cells
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Unstable EBV latency drives inflammation in multiple sclerosis patient derived spontaneous B cells
Authors: S Soldan, C Su, MC Monaco, N Brown, A Clauze, F Andrada, A Feder, P Planet, A Kossenkov, D Schäffer, J Ohayon, N Auslander, S Jacobson, P Lieberman
Research square, 2023-02-01;0(0):.
Species: Human
Sample Types: Whole Cells
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Immunogenicity and Efficacy of a Novel Multi-Antigenic Peptide Vaccine Based on Cross-Reactivity between Feline and Human Immunodeficiency Viruses
Authors: B Sahay, AM Aranyos, M Mishra, AC McAvoy, MM Martin, R Pu, S Shiomitsu, K Shiomitsu, MJ Dark, MP Sanou, SR Roff, MH Rathore, JK Yamamoto
Viruses, 2019-02-03;11(2):.
Species: Human
Sample Types: Whole Cells
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Autologous melanoma vaccine induces antitumor and self-reactive immune responses that affect patient survival and depend on MHC class II expression on vaccine cells.
Authors: Lotem M, Machlenkin A, Hamburger T, Nissan A, Kadouri L, Frankenburg S, Gimmon Z, Elias O, David IB, Kuznetz A, Shiloni E, Peretz T
Clin. Cancer Res., 2009-07-14;15(15):4968-77.
Species: Human
Sample Types: Whole Cells
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A neoadjuvant/adjuvant randomized trial of colorectal cancer patients vaccinated with an anti-idiotypic antibody, 105AD7, mimicking CD55.
Authors: Ullenhag GJ, Spendlove I, Watson NF, Indar AA, Dube M, Robins RA, Maxwell-Armstrong C, Scholefield JH, Durrant LG
Clin. Cancer Res., 2006-11-22;12(24):7389-96.
Species: Human
Sample Types: Whole Cells
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Intratumoral CD4+CD25+ regulatory T-cell-mediated suppression of infiltrating CD4+ T cells in B-cell non-Hodgkin lymphoma.
Authors: Yang ZZ, Witzig TE
Blood, 2006-01-10;107(9):3639-46.
Species: Human
Sample Types: Whole Cells
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Frequency of measles virus-specific CD4+ and CD8+ T cells in subjects seronegative or highly seropositive for measles vaccine.
Authors: Ovsyannikova IG, 107289, Dhiman N, Jacobson RM, Vierkant RA, Poland GA
SH005S7 Overcomes Primary and Acquired Resistance of Non-Small Cell Lung Cancer by Combined MET/EGFR/HER3 Inhibition, 2003-05-01;10(3):411-6.
Species: Human
Sample Types: Whole Cells
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