Human IFN-alpha / beta R1 Antibody

Catalog #: MAB2451 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB2451-100
MAB2451-SP

Detection of Human IFN‑ alpha / beta R1 by Western Blot.

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Human IFN-alpha / beta R1 Antibody Summary

Specificity

Detects human IFN-alpha / beta R1 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1056928

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IFN-alpha / beta R1
Lys28-Lys436
Accession # AAA52730

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

HeLa human cervical epithelial carcinoma cell line.

Flow Cytometry

25 µg/mL

U937 human histiocytic lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IFN‑ alpha / beta R1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IFN‑ alpha / beta R1 Monoclonal Antibody (Catalog # MAB2451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IFN‑ alpha / beta R1 at approximately 110-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry

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Detection of IFN‑ alpha / beta R1 in U937 human histiocytic lymphoma cell line by Flow Cytometry. U937 human histiocytic lymphoma cells were stained with Mouse Anti-Human IFN‑ alpha / beta R1 Monoclonal Antibody (Catalog # MAB2451, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background:

Type I interferons (IFN-alpha, IFN-beta, IFN-omega ) bind to the type I IFN receptor, also called the IFN alpha/beta receptor. This receptor is composed of two chains, IFN-alpha / beta R1 and R2.

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