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Human ICAM-1/CD54 Fluorescein-conjugated Antibody

Catalog #: BBA20
29 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
BBA20
Detection of ICAM‑1/CD54 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (29)
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Reviews (1)
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Human ICAM-1/CD54 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human ICAM-1/CD54 on human ICAM-1 transfected COS cells. It does not bind COS cells transfected with E-Selectin, VCAM-1, or PECAM-1.
Source
Monoclonal Mouse IgG1 Clone # BBIG-I1 (11C81)
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ICAM-1/CD54 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of ICAM‑1/CD54 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human ICAM-1/CD54 Fluorescein-conjugated Monoclonal Antibody (Catalog # BBA20) or (B) Mouse IgG1Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Human ICAM-1/CD54 by Flow Cytometry View Larger

Detection of Human ICAM-1/CD54 by Flow Cytometry EC and PC ICAM-1 expression and effects of CD18 blocking on neutrophil adhesion.ICAM-1 expression on (A) EC or (D) PC monolayers before and after 4 hr IL-1 beta activation was assessed by flow cytometry (IgG labeled control white). ICAM-1 expression on (B,C) EC and (E,F) PC following 4 hr IL-1 beta activation was imaged using confocal microscopy. Scale bars are 20 µm. (G) Neutrophil adhesion inhibition to IL-1 beta activated EC or PC monolayers. Freshly isolated neutrophils were pre-incubated with anti-Mac-1 or anti-LFA-1 antibodies and seeded onto EC or PC monolayers (activated for 4 with IL-1 beta ) in Sykes-Moore chambers and allowed to adhere prior to counting. Bars represent average neutrophil adhesion ± SEM. *P<0.05, when compared to the no block control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0060025), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Human ICAM-1/CD54 by Flow Cytometry View Larger

Detection of Human ICAM-1/CD54 by Flow Cytometry EC and PC ICAM-1 expression and effects of CD18 blocking on neutrophil adhesion.ICAM-1 expression on (A) EC or (D) PC monolayers before and after 4 hr IL-1 beta activation was assessed by flow cytometry (IgG labeled control white). ICAM-1 expression on (B,C) EC and (E,F) PC following 4 hr IL-1 beta activation was imaged using confocal microscopy. Scale bars are 20 µm. (G) Neutrophil adhesion inhibition to IL-1 beta activated EC or PC monolayers. Freshly isolated neutrophils were pre-incubated with anti-Mac-1 or anti-LFA-1 antibodies and seeded onto EC or PC monolayers (activated for 4 with IL-1 beta ) in Sykes-Moore chambers and allowed to adhere prior to counting. Bars represent average neutrophil adhesion ± SEM. *P<0.05, when compared to the no block control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0060025), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human ICAM-1/CD54 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human ICAM-1/CD54 by Immunocytochemistry/Immunofluorescence EC and PC ICAM-1 expression and effects of CD18 blocking on neutrophil adhesion.ICAM-1 expression on (A) EC or (D) PC monolayers before and after 4 hr IL-1 beta activation was assessed by flow cytometry (IgG labeled control white). ICAM-1 expression on (B,C) EC and (E,F) PC following 4 hr IL-1 beta activation was imaged using confocal microscopy. Scale bars are 20 µm. (G) Neutrophil adhesion inhibition to IL-1 beta activated EC or PC monolayers. Freshly isolated neutrophils were pre-incubated with anti-Mac-1 or anti-LFA-1 antibodies and seeded onto EC or PC monolayers (activated for 4 with IL-1 beta ) in Sykes-Moore chambers and allowed to adhere prior to counting. Bars represent average neutrophil adhesion ± SEM. *P<0.05, when compared to the no block control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0060025), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human ICAM-1/CD54 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human ICAM-1/CD54 by Immunocytochemistry/Immunofluorescence EC and PC ICAM-1 expression and effects of CD18 blocking on neutrophil adhesion.ICAM-1 expression on (A) EC or (D) PC monolayers before and after 4 hr IL-1 beta activation was assessed by flow cytometry (IgG labeled control white). ICAM-1 expression on (B,C) EC and (E,F) PC following 4 hr IL-1 beta activation was imaged using confocal microscopy. Scale bars are 20 µm. (G) Neutrophil adhesion inhibition to IL-1 beta activated EC or PC monolayers. Freshly isolated neutrophils were pre-incubated with anti-Mac-1 or anti-LFA-1 antibodies and seeded onto EC or PC monolayers (activated for 4 with IL-1 beta ) in Sykes-Moore chambers and allowed to adhere prior to counting. Bars represent average neutrophil adhesion ± SEM. *P<0.05, when compared to the no block control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0060025), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human ICAM-1/CD54 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human ICAM-1/CD54 by Immunocytochemistry/Immunofluorescence EC and PC ICAM-1 expression and effects of CD18 blocking on neutrophil adhesion.ICAM-1 expression on (A) EC or (D) PC monolayers before and after 4 hr IL-1 beta activation was assessed by flow cytometry (IgG labeled control white). ICAM-1 expression on (B,C) EC and (E,F) PC following 4 hr IL-1 beta activation was imaged using confocal microscopy. Scale bars are 20 µm. (G) Neutrophil adhesion inhibition to IL-1 beta activated EC or PC monolayers. Freshly isolated neutrophils were pre-incubated with anti-Mac-1 or anti-LFA-1 antibodies and seeded onto EC or PC monolayers (activated for 4 with IL-1 beta ) in Sykes-Moore chambers and allowed to adhere prior to counting. Bars represent average neutrophil adhesion ± SEM. *P<0.05, when compared to the no block control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0060025), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human ICAM-1/CD54 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human ICAM-1/CD54 by Immunocytochemistry/Immunofluorescence EC and PC ICAM-1 expression and effects of CD18 blocking on neutrophil adhesion.ICAM-1 expression on (A) EC or (D) PC monolayers before and after 4 hr IL-1 beta activation was assessed by flow cytometry (IgG labeled control white). ICAM-1 expression on (B,C) EC and (E,F) PC following 4 hr IL-1 beta activation was imaged using confocal microscopy. Scale bars are 20 µm. (G) Neutrophil adhesion inhibition to IL-1 beta activated EC or PC monolayers. Freshly isolated neutrophils were pre-incubated with anti-Mac-1 or anti-LFA-1 antibodies and seeded onto EC or PC monolayers (activated for 4 with IL-1 beta ) in Sykes-Moore chambers and allowed to adhere prior to counting. Bars represent average neutrophil adhesion ± SEM. *P<0.05, when compared to the no block control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0060025), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ICAM-1/CD54

ICAM-1 is a member of the immunoglobulin superfamily whose expression is up‑regulated on leukocytes, epithelial cells and resting endothelial cells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.

Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

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Citations for Human ICAM-1/CD54 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. APOL1 promotes endothelial cell activation beyond the glomerulus
    Authors: Miguel Carracedo, Elke Ericson, Rasmus Ågren, Anna Forslöw, Katja Madeyski-Bengtson, Anna Svensson et al.
    iScience
  2. Decidua Parietalis Mesenchymal Stem/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In Vitro
    Authors: Y Basmaeil, E Bahattab, A Al Subayyi, HB Kulayb, M Alrodayyan, M Abumaree, T Khatlani
    Cells, 2021-12-10;10(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Epigenetic modulation of immune synaptic-cytoskeletal networks potentiates &gamma&delta T cell-mediated cytotoxicity in lung cancer
    Authors: RR Weng, HH Lu, CT Lin, CC Fan, RS Lin, TC Huang, SY Lin, YJ Huang, YH Juan, YC Wu, ZC Hung, C Liu, XH Lin, WC Hsieh, TY Chiu, JC Liao, YL Chiu, SY Chen, CJ Yu, HC Tsai
    Nature Communications, 2021-04-12;12(1):2163.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Anti-inflammatory Effects of Alcohol Are Associated with JNK-STAT3 Downregulation in an In Vitro Inflammation Model in HepG2 Cells
    Authors: K Mörs, R Sturm, JA Hörauf, S Kany, P Cavalli, J Omari, M Powerski, A Surov, I Marzi, AJ Nowak, B Relja
    Disease Markers, 2021-03-18;2021(0):6622701.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. LasR-deficient Pseudomonas aeruginosa variants increase airway epithelial mICAM-1 expression and enhance neutrophilic lung inflammation
    Authors: LC Hennemann, SL LaFayette, JK Malet, P Bortolotti, T Yang, GA McKay, D Houle, D Radzioch, S Rousseau, D Nguyen
    PloS Pathogens, 2021-03-10;17(3):e1009375.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnusrhamnoides (L.) A. Nelson-The Implications for Human Barrier Cells
    Authors: B Sadowska, J Rywaniak, A Cichocka, K Cichocka, J ?uchowski, U Wójcik-Boj, M Wi?ckowska, B Ró?alska
    Molecules, 2020-05-09;25(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Effects of cisplatin on the proliferation, invasion and apoptosis of breast cancer cells following beta ‑catenin silencing
    Authors: Xidan Zhu, Jia Feng, Wenguang Fu, Xiaojia Shu, Xue Wan, Jinbo Liu
    International Journal of Molecular Medicine
  8. Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps
    Authors: L Tengroth, J Arebro, O Larsson, C Bachert, SK Georén, LO Cardell
    Sci Rep, 2018-01-24;8(1):1561.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. SIRT1 inhibition causes oxidative stress and inflammation in patients with coronary artery disease
    Authors: SH Chan, CH Hung, JY Shih, PM Chu, YH Cheng, HC Lin, KL Tsai
    Redox Biol, 2017-06-02;13(0):301-309.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Endothelial CD2AP Binds the Receptor ICAM-1 To Control Mechanosignaling, Leukocyte Adhesion, and the Route of Leukocyte Diapedesis In Vitro
    Authors: A Schaefer, TJ van Duijn, J Majolee, K Burridge, PL Hordijk
    J. Immunol., 2017-05-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Tranexamic acid and the gut barrier: Protection by inhibition of trypsin uptake and activation of downstream intestinal proteases
    Authors: ME Diebel, LN Diebel, DM Liberati
    Am. J. Surg, 2016-12-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Prenylated Polyphenols from Clusiaceae and Calophyllaceae with Immunomodulatory Activity on Endothelial Cells
    PLoS ONE, 2016-12-01;11(12):e0167361.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles
    Authors: KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck
    J. Biol. Chem., 2016-03-22;291(20):10586-601.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Ethanol, ethyl and sodium pyruvate decrease the inflammatory responses of human lung epithelial cells via Akt and NF-kappa B in vitro but have a low impact on hepatocellular cells
    Authors: B. RELJA, N. OMID, N. WAGNER, K. MÖRS, I. WERNER, E. JUENGEL et al.
    International Journal of Molecular Medicine
  15. Pre- or post-treatment with ethanol and ethyl pyruvate results in distinct anti-inflammatory responses of human lung epithelial cells triggered by interleukin-6
    Authors: BORNA RELJA, NINA OMID, ALEXANDER SCHAIBLE, MARIO PERL, SIMON MEIER, ELSIE OPPERMANN et al.
    Molecular Medicine Reports
  16. Molecular mechanisms regulating the synergism between IL-32gamma and NOD for the activation of eosinophils.
    Authors: Wong C, Dong J, Lam C
    J Leukoc Biol, 2013-12-02;95(4):631-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Transendothelial migration enables subsequent transmigration of neutrophils through underlying pericytes.
    Authors: Ayres-Sander C, Lauridsen H, Maier C, Sava P, Pober J, Gonzalez A
    PLoS ONE, 2013-03-26;8(3):e60025.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Distinct binding affinities of Mac-1 and LFA-1 in neutrophil activation.
    Authors: Li N, Mao D, Lu S, Tong C, Zhang Y, Long M
    J Immunol, 2013-03-20;190(8):4371-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Anti-inflammatory and anti-allergic activities of Pentaherb formula, Moutan Cortex (Danpi) and gallic acid.
    Authors: Liu K, Hu S, Chan B, Wat E, Lau C, Hon K, Fung K, Leung P, Hui P, Lam C, Wong C
    Molecules, 2013-02-25;18(3):2483-500.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. TWEAK/Fn14 pathway modulates properties of a human microvascular endothelial cell model of blood brain barrier.
    Authors: Stephan D, Sbai O, Wen J, Couraud P, Putterman C, Khrestchatisky M, Desplat-Jego S
    J Neuroinflammation, 2013-01-15;10(0):9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. IL-22 suppresses IFN-gamma-mediated lung inflammation in asthmatic patients.
    Authors: Pennino D, Bhavsar P, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham S, Cavani A, Eyerich K, Chung K, Schmidt-Weber C, Eyerich S
    J Allergy Clin Immunol, 2012-11-19;131(2):562-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Activation of endothelial TLR2 by bacterial lipoprotein upregulates proteins specific for the neutrophil response
    Authors: Kevin Wilhelmsen, Kailin R Mesa, Arun Prakash, Fengyun Xu, Judith Hellman
    Innate Immunity
  23. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
    Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
    J Biol Chem, 2012-06-15;287(32):26478-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Endothelial inflammation correlates with subject triglycerides and waist size after a high-fat meal
    Authors: Ying I. Wang, John Schulze, Nadine Raymond, Tyler Tomita, Kayan Tam, Scott I. Simon et al.
    American Journal of Physiology-Heart and Circulatory Physiology
  25. Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
    Authors: Haarmann A, Deiss A, Prochaska J, Foerch C, Weksler B, Romero I, Couraud PO, Stoll G, Rieckmann P, Buttmann M
    PLoS ONE, 2010-10-21;5(10):e13568.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. IL-6 regulates MCP-1, ICAM-1 and IL-6 expression in human myoblasts.
    Authors: Marino M, Scuderi F, Provenzano C, Scheller J, Rose-John S, Bartoccioni E
    J. Neuroimmunol., 2008-04-08;196(1):41-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Dehydroepiandrosterone inhibits the TNF-alpha-induced inflammatory response in human umbilical vein endothelial cells.
    Authors: Gutierrez G, Mendoza C, Zapata E, Montiel A, Reyes E, Montano LF, Lopez-Marure R
    Atherosclerosis, 2006-03-30;190(1):90-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. NF-kappaB-dependent lymphocyte hyperadhesiveness to synovial fibroblasts by hypoxia and reoxygenation: potential role in rheumatoid arthritis.
    Authors: Han MK, Kim JS, Park BH, Kim JR, Hwang BY, Lee HY, Song EK, Yoo WH
    J. Leukoc. Biol., 2003-04-01;73(4):525-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. Triglyceride-rich lipoprotein modulates endothelial vascular cell adhesion molecule (VCAM)-1 expression via differential regulation of endoplasmic reticulum stress.
    Authors: Wang YI, Bettaieb A, Sun C et al.
    PLoS One.

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Human ICAM-1/CD54 Fluorescein-conjugated Antibody
By Anonymous on 07/07/2017
Application: Flow Sample Tested: Peripheral blood cells Species: Human