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Human ICAM-1/CD54 Biotinylated Antibody

Catalog #: BBA9
6 Citations Datasheet
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BBA9
Product Details
Citations (6)
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Human ICAM-1/CD54 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAM-1 in Western blots. In this format, this antibody does not cross‑react with rhCEACAM-1, rhICAM-2, -3, -5, rmICAM-2, rrICAM-1, rmMAdCAM, or rhVCAM-1.
Source
Monoclonal Mouse IgG1 Clone # BBIG-I1 (11C81)
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated human umbilical vein endothelial cells (HUVEC)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ICAM‑1/CD54 Fc Chimera (Catalog # 720-IC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

ICAM-1 is a member of the immunoglobulin superfamily whose expression is upregulated on leukocytes, epithelial cells, and resting endothelial cells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.

Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

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Citations for Human ICAM-1/CD54 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Modified two-step emulsion solvent evaporation technique for fabricating biodegradable rod-shaped particles in the submicron size range
    Authors: H Safari, R Adili, M Holinstat, O Eniola-Ade
    J Colloid Interface Sci, 2018-02-09;518(0):174-183.
    Species: Human
    Sample Types: Microparticles, Whole Cells
    Applications: Flow Cytometry, Functional Assay
  2. Annexin A2 Limits Neutrophil Transendothelial Migration by Organizing the Spatial Distribution of ICAM-1.
    Authors: Heemskerk N, Asimuddin M, Oort C, van Rijssel J, van Buul J
    J Immunol, 2016-02-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Plasma protein corona modulates the vascular wall interaction of drug carriers in a material and donor specific manner.
    Authors: Sobczynski D, Charoenphol P, Heslinga M, Onyskiw P, Namdee K, Thompson A, Eniola-Adefeso O
    PLoS ONE, 2014-09-17;9(9):e107408.
    Applications: Bioassay
  4. Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
    Authors: Arnold R, Neumann M, Konig W
    Immunology, 2007-05-01;121(1):71-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Adhesion Blockade
  5. Endothelium properties of a tissue-engineered blood vessel for small-diameter vascular reconstruction.
    Authors: Remy-Zolghadri M, Laganiere J, Oligny JF, Germain L, Auger FA
    J. Vasc. Surg., 2004-03-01;39(3):613-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling
    Authors: Niels Heemskerk, Lilian Schimmel, Chantal Oort, Jos van Rijssel, Taofei Yin, Bin Ma et al.
    Nature Communications

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