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Human ICAM-1/CD54 Alexa Fluor® 532-conjugated Antibody

Catalog #: BBA3X Datasheet
Catalog # Availability Size / Price Qty
BBA3X-100UG
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Human ICAM-1/CD54 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity
Human
Specificity
This antibody was selected for its ability to stain human ICAM-1/CD54 transfected COS cells. It does not stain COS cells transfected with human E-Selectin, VCAM-1, or PECAM-1.
Source
Monoclonal Mouse IgG1 Clone # BBIG-I1 (11C81)
Purification
Protein A or G purified
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
 
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
 
Adhesion Blockade
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ICAM-1/CD54

ICAM-1 is a member of the immunoglobulin superfamily whose expression is upregulated on leukocytes, epithelial cells and resting endothelial cells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.

Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

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