Human HB-EGF Biotinylated Antibody Summary
Asp63-Leu148
Accession # Q99075
Applications
Human HB-EGF Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HB-EGF
HB-EGF was originally purified based on its heparin-binding property and mitogenic activity on BALB-3T3 fibroblasts from the conditioned medium of the human U-937 histiocytic lymphoma cell line. The natural protein has an apparent molecular mass of 19 - 23 kDa and exists in multiple forms as a result of heterogenous O‑glycosylation and/or N-terminal truncation. In addition to fibroblasts, HB-EGF is also a potent mitogen for keratinocytes and smooth muscle cells but not for capillary endothelial cells. HB-EGF is produced in monocytes and macrophages. In addition, transcription of HB-EGF can be induced in vascular endothelial cells as well as aortic smooth muscle cells (SMC), suggesting that HB-EGF may have an important role in the pathogenesis of atherosclerosis.
HB-EGF is a member of the EGF family of mitogens which also include transforming growth factor-alpha (TGF-alpha ), amphiregulin (AR), rat schwanoma-derived growth factor (SDGF), vaccinia growth factor (VGF), and the various ligands for the HER2/erbB2/neu receptor. All these cytokines are derived from transmembrane precursors that contain one or several EGF structural units in their extracellular domain. Many of these transmembrane precursors are biologically active and seem to play a role in juxtacrine stimulation of adjacent cells. The cDNA for HB-EGF encodes a 204 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB-EGF. Like EGF, TGF-alpha, and AR; HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. HB-EGF is reported to be a more potent SMC mitogen than EGF. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF. A diphtheria toxin receptor that mediates the endocytosis of the bound toxin has been cloned and found to be identical to the transmembrane HB-EGF precursor.
Product Datasheets
Citations for Human HB-EGF Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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A stromal niche defined by expression of the transcription factor Wilms tumor 1 mediates programming and homeostasis of cavity-resident macrophages
Authors: Matthew B. Buechler, Ki-Wook Kim, Emily J. Onufer, Jesse W. Williams, Christine C. Little, Claudia X. Dominguez et al.
Immunity
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Altered lymph node composition in diphtheria toxin receptor-based mouse models to ablate dendritic cells.
Authors: van Blijswijk J, Schraml B, Rogers N, Whitney P, Zelenay S, Acton S, Reis E Sousa C
J Immunol, 2014-11-19;194(1):307-15.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Ovarian cancer cell heparan sulfate 6-O-sulfotransferases regulate an angiogenic program induced by heparin-binding epidermal growth factor (EGF)-like growth factor/EGF receptor signaling.
Authors: Cole C, Rushton G, Jayson G, Avizienyte E
J Biol Chem, 2014-02-22;289(15):10488-501.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Development (Detection) -
Chronic follicular bronchiolitis requires antigen-specific regulatory T cell control to prevent fatal disease progression.
Authors: Schmitt E, Haribhai D, Jeschke J, Co D, Ziegelbauer J, Yan K, Iwakura Y, Mishra M, Simpson P, Salzman N, Williams C
J Immunol, 2013-10-25;191(11):5460-76.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development -
Regulatory T cells prevent catastrophic autoimmunity throughout the lifespan of mice.
Authors: Kim JM, Rasmussen JP, Rudensky AY
Nat. Immunol., 2006-11-30;8(2):191-7.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The response of human epithelial cells to TNF involves an inducible autocrine cascade.
Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
Cell, 2006-03-24;124(6):1225-39.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Primary germinal center-resident T follicular helper cells are a physiologically distinct subset of CXCR5hiPD-1hi T follicular helper cells
Authors: Chen-Hao Yeh, Joel Finney, Takaharu Okada, Tomohiro Kurosaki, Garnett Kelsoe
Immunity
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