Human Granzyme B PE-conjugated Antibody Summary
Gly19-Tyr247
Accession # P10144
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Granzyme B in NK‑92 Human Cell Line by Flow Cytometry. NK-92 human natural killer lymphoma cell line was stained with Mouse Anti-Human Granzyme B PE-conjugated Monoclonal Antibody (Catalog # IC2906P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Granzyme B
Granzyme B is a member of the Granzyme family of the serine proteases found specifically in the cytotoxic granules of Cytotoxic T Lymphocytes (CTL) and Natural Killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various Caspases and cleaves proteins such as Aggrecan (3). Human Granzyme B is synthesized as a 247 amino acid (aa) precursor with a signal peptide (aa 1-18), a pro peptide (aa 19-20), and a mature chain (aa 21-247) (4-6). The recombinant human (rh) Granzyme B consisting of aa 19-247 was expressed and purified. After being activated by active Cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3).
- Kam, C-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
- Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
- Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al., eds., pp. 1549 - 1552.
- Schmid, J. and C. Weissman (1987) J. Immunol. 139:250.
- Caputo, A. et al. (1988) J. Biol. Chem. 263:6363.
- Trapani, J.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6924.
Product Datasheets
Citations for Human Granzyme B PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Transcriptomic analysis of human IL-7 receptor alpha low and high effector memory CD8+ T cells reveals an age-associated signature linked to influenza vaccine response in older adults
Authors: HJ Park, MS Shin, M Kim, JB Bilsborrow, S Mohanty, RR Montgomery, AC Shaw, S You, I Kang
Aging Cell, 2019-05-01;0(0):e12960.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Anti-CD137 monoclonal antibody enhances trastuzumab-induced, natural killer cell-mediated cytotoxicity against pancreatic cancer cell lines with low human epidermal growth factor-like receptor 2 expression
Authors: T Masu, M Atsukawa, K Nakatsuka, M Shimizu, D Miura, T Arai, H Harimoto, C Kondo, K Kaneko, S Futagami, C Kawamoto, H Takahashi, K Iwakiri
PLoS ONE, 2018-12-31;13(12):e0200664.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Humoral and cell-mediated immune responses after a booster dose of HBV vaccine in HIV-infected children, adolescents and young adults
Authors: V Giacomet, M Masetti, P Nannini, F Forlanini, M Clerici, GV Zuccotti, D Trabattoni
PLoS ONE, 2018-02-14;13(2):e0192638.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
Authors: Dorneles E, Lima G, Teixeira-Carvalho A, Araujo M, Martins-Filho O, Sriranganathan N, Al Qublan H, Heinemann M, Lage A
PLoS ONE, 2015-09-09;10(9):e0136696.
Species: Bovine
Sample Types: Whole Cells
Applications: Flow Cytometry -
High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer.
Authors: Peguillet I, Milder M, Louis D, Vincent-Salomon A, Dorval T, Piperno-Neumann S, Scholl S, Lantz O
Cancer Res, 2014-02-17;74(8):2204-16.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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