Human Granzyme B Antibody

Catalog #: MAB105409 Datasheet

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB105409-100
MAB105409-SP

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All Granzyme B Antibodies

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Human Granzyme B Antibody Summary

Species Reactivity

Human

Specificity

Detects human Granzyme B in sandwich ELISAs.

Source

Recombinant Monoclonal Human IgG Clone # 2103D

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Granzyme B
Gly19-Tyr247
Accession # P10144

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Human Granzyme B Monoclonal Antibody (Catalog # MAB105408).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Granzyme B DuoSet ELISA Kit (Catalog # DY2906-05) for convenient development of a sandwich ELISA or the Human Granzyme B Quantikine ELISA Kit (Catalog # DGZB00) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Granzyme B ELISA Standard Curve. Recombinant Human Granzyme B protein was serially diluted 2-fold and captured by Human Anti-Human Granzyme B Monoclonal Antibody (Catalog # MAB105409) coated on a Clear Polystyrene Microplate (DY990). Rabbit Anti-Human Granzyme B Monoclonal Antibody(MAB105408) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme B

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Human Granzyme B is synthesized as a precursor (247 residues) with a signal peptide (residues 1-18), a pro peptide (residues 19-20), and a mature chain (residues 21-247) (4-6). The recombinant human (rh) Granzyme B consisting of residues 19-247 was expressed and purified. After being activated by active cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3).

References

Kam, C-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1549.
Schmid, J. and C. Weissman (1987) J. Immunol. 139:250.
Caputo, A. et al. (1988) J. Biol. Chem. 263:6363.
Trapani, J.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6924.