Human Granzyme B Antibody

Catalog #: MAB105408 Datasheet

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB105408-100
MAB105408-SP

2 Images

All Granzyme B Antibodies

Product Details

FAQs

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Granzyme B Antibody Summary

Species Reactivity

Human

Specificity

Detects human Granzyme B in sandwich ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2103C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Granzyme B
Gly19-Tyr247
Accession # P10144

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

NK‑92 human natural killer lymphoma cell line

ELISA

This antibody functions as an ELISA detection antibody when paired with Human Anti-Human Granzyme B Monoclonal Antibody (Catalog # MAB105409).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Granzyme B DuoSet ELISA Kit (Catalog # DY2906-05) for convenient development of a sandwich ELISA or the Human Granzyme B Quantikine ELISA Kit (Catalog # DGZB00) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

View Larger

Human Granzyme B ELISA Standard Curve. Recombinant Human Granzyme B protein was serially diluted 2-fold and captured by Human Anti-Human Granzyme B Monoclonal Antibody (MAB105409) coated on a Clear Polystyrene Microplate (DY990). Rabbit Anti-Human Granzyme B Monoclonal Antibody (Catalog # MAB105408) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Western Blot

View Larger

Detection of Human Granzyme B by Western Blot. Western blot shows lysates of NK‑92 human natural killer lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Granzyme B Monoclonal Antibody (Catalog # MAB105408) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Granzyme B at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme B

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Human Granzyme B is synthesized as a precursor (247 residues) with a signal peptide (residues 1-18), a pro peptide (residues 19-20), and a mature chain (residues 21-247) (4-6). The recombinant human (rh) Granzyme B consisting of residues 19-247 was expressed and purified. After being activated by active cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3).

References

Kam, C-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1549.
Schmid, J. and C. Weissman (1987) J. Immunol. 139:250.
Caputo, A. et al. (1988) J. Biol. Chem. 263:6363.
Trapani, J.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6924.