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Human GPER/GPR30 Antibody

Catalog #: AF5534
9 Citations Datasheet
Catalog # Availability Size / Price Qty
AF5534
AF5534-SP
Detection of Human GPER by Western Blot.
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Product Details
Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPER/GPR30 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPER in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human GPR144, GPR115, and GPR124 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GPER
Met1-Ser62
Accession # Q99527
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human GPER antibody by Western Blot. View Larger

Detection of Human GPER by Western Blot. Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GPER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5534) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GPER at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry Detection of GPER/GPR30 antibody in HEK293 Human Cell Line Transfected with human GPER/GRP30 and eGFP antibody by Flow Cytometry. View Larger

Detection of GPER/GPR30 in HEK293 Human Cell Line Transfected with human GPER/GRP30 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human GPER/GRP30 or (B) irrelevant transfectants, and eGFP was stained with Goat Anti-Human GPER/GPR30 Affinity-Purified Polyclonal Antibody (Catalog # AF5534) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). Quadrants were set based on Goat IgG Flow Cytometry Isotype Control (Catalog # AB-108-C, data not shown). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry GPER antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

GPER in Human Brain. GPER was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Goat Anti-Human GPER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5534) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPER/GPR30

GPER (G-Protein Coupled Estrogen Receptor 1; also GPR30, DRY12 and mER) is a 44 kDa, seven transmembrane (TM) member of the GPR-1 family of molecules. It is ubiquitously expressed, appearing on/in neurons, monocytes and endothelial cells. Its exact location is unclear; it has been described in both the cell membrane and ER, but not by all investigators. Human GPER is 375 amino acids (aa) in length. It contains an N-terminal extracellular region (aa 1‑62), a series of seven TM domains (aa 63‑327), and a C-terminal cytoplasmic tail (aa 328‑375). The initial function attributed to GPER was that of a membrane receptor for estrogen. This is in dispute. There are two potential splice variants for GPER. One shows a deletion of aa 32-49, while a second shows a 99 aa substitution for aa 308‑375. Over aa 1‑62, human GPER shares 57% aa identity with mouse GPER.

Long Name
G Protein-coupled Estrogen Receptor 1
Entrez Gene IDs
2852 (Human); 76854 (Mouse); 171104 (Rat)
Alternate Names
CEPR; CEPRG-protein coupled receptor 30; Chemoattractant receptor-like 2; chemokine receptor-like 2; CMKRL2; CMKRL2MGC99678; constitutively expressed peptide-like receptor; DRY12; DRY12IL8-related receptor DRY12; FEG-1; FEG-1mER; Flow-induced endothelial G-protein coupled receptor 1; G protein-coupled estrogen receptor 1; G protein-coupled receptor 30; GPCR-BR; GPER; GPR30; GPR30GPCR-Br; G-protein coupled estrogen receptor 1; heptahelix receptor; LERGU; LERGU2; leucine rich protein in GPR30 3'UTR; LyGPR; Lymphocyte-derived G-protein coupled receptor; Membrane estrogen receptor

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Citations for Human GPER/GPR30 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Single-Molecule Imaging Reveals Rapid Estradiol Action on the Surface Movement of AMPA Receptors in Live Neurons
    Authors: Soma Godó, Klaudia Barabás, Ferenc Lengyel, Dávid Ernszt, Tamás Kovács, Miklós Kecskés et al.
    Frontiers in Cell and Developmental Biology
  2. Estrogen Promotes Pro-resolving Microglial Behavior and Phagocytic Cell Clearance Through the Actions of Annexin A1
    Authors: Rodrigo Azevedo Loiola, Edward S. Wickstead, Egle Solito, Simon McArthur
    Front Endocrinol (Lausanne)
  3. The G protein-coupled estrogen receptor 1 (GPER/GPR30) does not predict survival in patients with ovarian cancer.
    Authors: Kolkova Z, Casslen V, Henic E, Ahmadi S, Ehinger A, Jirstrom K, Casslen B
    J Ovarian Res, 2012-03-18;5(0):9.
  4. Assessment of G Protein-Coupled Oestrogen Receptor Expression in Normal and Neoplastic Human Tissues Using a Novel Rabbit Monoclonal Antibody
    Authors: M Bubb, AL Beyer, P Dasgupta, D Kaemmerer, J Sänger, K Evert, RM Wirtz, S Schulz, A Lupp
    International Journal of Molecular Sciences, 2022-05-06;23(9):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Plasma membrane expression of G protein-coupled estrogen receptor (GPER)/G protein-coupled receptor 30 (GPR30) is associated with worse outcome in metachronous contralateral breast cancer
    Authors: J Tutzauer, M Sjöström, PO Bendahl, L Rydén, M Fernö, LMF Leeb-Lundb, S Alkner
    PLoS ONE, 2020-04-17;15(4):e0231786.
    Species: Human
    Sample Types: Cell Lysates
  6. G protein-coupled receptor 30 (GPR30) forms a plasma membrane complex with membrane-associated guanylate kinases (MAGUKs) and protein kinase A-anchoring protein 5 (AKAP5) that constitutively inhibits cAMP production.
    Authors: Broselid S, Berg K, Chavera T, Kahn R, Clarke W, Olde B, Leeb-Lundberg L
    J Biol Chem, 2014-06-24;289(32):22117-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr, Western Blot
  7. Cytoplasmic G Protein-Coupled Estrogen Receptor 1 as a Prognostic Indicator of Breast Cancer: A Meta-Analysis
    Authors: Duo Zhang, Jinpeng Wang, Hong Chen, Shunchao Yan
    Technol Cancer Res Treat
  8. GPER mediates differential effects of estrogen on colon cancer cell proliferation and migration under normoxic and hypoxic conditions
    Authors: Viviana Bustos, Áine M. Nolan, Anke Nijhuis, Harry Harvey, Alexandra Parker, Richard Poulsom et al.
    Oncotarget
  9. G protein-coupled estrogen receptor 1 (GPER, GPR 30) in normal human endometrium and early pregnancy decidua
    Authors: Z. Kolkova, V. Noskova, A. Ehinger, S. Hansson, B. Casslen
    Molecular Human Reproduction

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