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Human GPER/GPR30 Antibody

Catalog #: MAB5534
1 Citation Datasheet
Catalog # Availability Size / Price Qty
MAB5534
MAB5534-SP
GPER in Human Brain.
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Citations (1)
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Human GPER/GPR30 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPER in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 730737
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GPER
Met1-Ser62
Accession # Q99527
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry GPER antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

GPER in Human Brain. GPER was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human GPER Monoclonal Antibody (Catalog # MAB5534) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPER/GPR30

GPER (G-Protein Coupled Estrogen Receptor 1; also GPR30, DRY12 and mER) is a 44 kDa, seven transmembrane (TM) member of the GPR-1 family of molecules. It is expressed on/in neurons, monocytes and endothelial cells. Its exact location is unclear; it has been described in both the cell membrane and ER. Human GPER is 375 amino acids (aa) in length. It contains an N-terminal extracellular region (aa 1‑62), a series of seven TM domains (aa 63‑327), and a C-terminal cytoplasmic tail (aa 328‑375). The initial function attributed to GPER was that of a membrane receptor for estrogen. There are two potential splice variants for GPER. One shows a deletion of aa 32-49, while a second shows a 99 aa substitution for aa 308‑375. Over aa 1‑62, human GPER shares 57% aa identity with mouse GPER.

Long Name
G Protein-coupled Estrogen Receptor 1
Entrez Gene IDs
2852 (Human); 76854 (Mouse); 171104 (Rat)
Alternate Names
CEPR; CEPRG-protein coupled receptor 30; Chemoattractant receptor-like 2; chemokine receptor-like 2; CMKRL2; CMKRL2MGC99678; constitutively expressed peptide-like receptor; DRY12; DRY12IL8-related receptor DRY12; FEG-1; FEG-1mER; Flow-induced endothelial G-protein coupled receptor 1; G protein-coupled estrogen receptor 1; G protein-coupled receptor 30; GPCR-BR; GPER; GPR30; GPR30GPCR-Br; G-protein coupled estrogen receptor 1; heptahelix receptor; LERGU; LERGU2; leucine rich protein in GPR30 3'UTR; LyGPR; Lymphocyte-derived G-protein coupled receptor; Membrane estrogen receptor

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Citation for Human GPER/GPR30 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Tissue-specific gene expression during productive HPV16 infection of cervical, foreskin and tonsil epithelium
    Authors: Chatterjee S, Kang SD, Alam S et al.
    J. Virol.

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