Human GIF Antibody

Catalog #: AF8060 Datasheet

Catalog #	Availability	Size / Price	Qty
AF8060
AF8060-SP

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Human GIF Antibody Summary

Species Reactivity

Human

Specificity

Detects human GIF in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human GIF
Ser19-Tyr417
Accession # P27352

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GIF by Western Blot. Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human GIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8060) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GIF at approx-imately 48-52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GIF

GIF (Gastric Intrinsic Factor; also better known as IF or Intrinsic Factor) is a 45-55 kDa, secreted glycoprotein member of the eukaryotic cobalamin transport family of proteins. It should not be confused with either GIF/growth inhibitory factor/metallothionein 3, or GIF/glycosylation-inhibiting factor/macrophage migration inhibitory factor/phenylpyruvate tautomerase. GIF shows restricted expression, being essentially limited to parietal and select chief cells of the stomach. GIF binds to haptocorrin-associated cobalamin/VitB12 in the duodenum, whereupon it passes into the ileum and is taken up by a 500 kDa epithelial receptor named cubam, a complex of cubilin and amnionless molecules. Mature human GIF is 399 amino acids (aa) in length (aa 19-417) that contains one principal B12 binding site between aa 365-395. There is one isoform variant that shows a deletion of aa 3-27. Full-length human GIF shares 82% aa sequence identity with mouse GIF.

Long Name

Gastric Intrinsic Factor

Entrez Gene IDs

2694 (Human); 14603 (Mouse); 29319 (Rat)

Alternate Names

gastric intrinsic factor (vitamin B synthesis); GIF; IF; IFIntrinsic factor; IFMH; IFMHgastric intrinsic factor; INF; TCN3

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