Human GDF-15 Quantikine QuicKit ELISA Summary
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (10 uL)
Assay Range
23.4 - 1,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Recognizes natural and recombinant human GDF-15
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the
presence of human GDF-15 in this assay. No medical histories were available for the donors
used in this study.
| Sample Type | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=10) | 556 | 204-1146 | 315 |
| EDTA plasma (n=10) | 511 | 249-1042 | 281 |
| Heparin plasma (n=10) | 539 | 243-1009 | 282 |
| Urine (n=4) | 4783 | 3243-8287 | 2353 |
Cell Culture Supernates:
HepG2 cells were cultured in MEM NEAA Earle's Salts supplemented with 10% fetal bovine
serum (FBS), 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until
nearly confluent. The cell conditioned media was taken off and centrifuged to remove any cells
or debris and stored at ≤ -20 °C. An aliquot of the cell culture supernate was removed, assayed
for human GDF-15, and measured at 47,216 pg/mL.
JAR cells were cultured in DMEM NEAA Earle's Salts supplemented with 10% FBS, 2 mM
L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until nearly confluent.
The cell conditioned media was taken off and centrifuged to remove any cells or debris and
stored at ≤ -20 °C. An aliquot of the cell culture supernate was removed, assayed for human
GDF-15, and measured at 29,417 pg/mL.
JEG-3 cells were cultured in MEM NEAA Earle's Salts supplemented with 10% FBS,
2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until nearly
confluent. The cell conditioned media was taken off and centrifuged to remove any cells or
debris and stored at ≤ -20 °C. An aliquot of the cell culture supernate was removed, assayed for
human GDF-15, and measured at 5651 pg/mL.
Product Summary
The Quantikine™ QuicKit™ Human GDF-15 Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human GDF-15 levels in cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human GDF-15 and antibodies raised against the recombinant protein. Results obtained using natural human GDF-15 showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human GDF-15.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
|
Intra-Assay Precision |
Inter-Assay Precision |
| Sample |
1 |
2 |
1 |
2 |
| n |
20 |
20 |
10 |
10 |
| Mean (pg/mL) |
156 |
882 |
165 |
925 |
| Standard Deviation |
6.12 |
58.8 |
9.49 |
77.6 |
| CV% |
3.9 |
6.7 |
5.7 |
8.4 |
Recovery
The recovery of human GDF-15 spiked to three levels in samples throughout the range of the assay was evaluated.
| Sample Type |
Average % Recovery |
Range % |
| Cell Culture Media (n=4) |
101 |
92-113 |
| EDTA Plasma (n=2) |
74 |
66-83 |
| Heparin Plasma (n=2) |
77 |
66-93 |
| Serum (n=2) |
77 |
66-84 |
| Urine (n=2) |
104 |
94-116 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human GDF-15 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Human GDF-15 Standard Curve
Product Datasheets
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Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: GDF-15
Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.
Long Name:
Growth Differentiation Factor 15
Entrez Gene IDs:
9518 (Human); 23886 (Mouse); 29455 (Rat)
Alternate Names:
GDF15; GDF-15; growth differentiation factor 15; growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC-1NSAID-activated gene 1 protein; MIC1Prostate differentiation factor; NAG-1; NAG-1NSAID-regulated gene 1 protein; NSAID (nonsteroidal inflammatory drug)-activated protein 1; PDF; PDFGDF-15; PLAB; PLABNRG-1; Placental bone morphogenetic protein; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta
Citation for Human GDF-15 Quantikine QuicKit ELISA
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