Human G-CSF Antibody Summary
Thr31-Pro204
Accession # NP_757373
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Recombinant Human G‑CSF by Western Blot. Western blot shows 25 ng of Recombinant Human G-CSF (Catalog # 214-CS) and Recombinant Mouse G-CSF (Catalog # 414-CS). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human G-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-214-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for G-CSF at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
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Cell Proliferation Induced by G‑CSF and Neutralization by Human G‑CSF Antibody. Recombinant Human G-CSF (Catalog # 214-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human G-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-214-NA). The ND50 is typically 8-48 ng/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: G-CSF
G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.
In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.
In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.
Product Datasheets
Citations for Human G-CSF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapy
Authors: J Li, A Wuethrich, AAI Sina, HH Cheng, Y Wang, A Behren, PN Mainwaring, M Trau
Nature Communications, 2021-02-17;12(1):1087.
Species: Human
Sample Types: Serum
Applications: ELISA Capture -
Enhanced non-viral gene delivery by coordinated endosomal release and inhibition of ?-tubulin deactylase
Nucleic Acids Res, 2017-04-07;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock?
Authors: Rojahn A, Brusletto B, Ovstebo R, Haug KB, Kierulf P, Brandtzaeg P
Crit. Care Med., 2008-09-01;36(9):2583-9.
Species: Human
Sample Types: Plasma
Applications: Immunodepletion -
Proinflammatory mediators elicit secretion of the intracellular B-lymphocyte stimulator pool (BLyS) that is stored in activated neutrophils: implications for inflammatory diseases.
Authors: Scapini P, Carletto A, Nardelli B, Calzetti F, Roschke V, Merigo F, Tamassia N, Pieropan S, Biasi D, Sbarbati A, Sozzani S, Bambara L, Cassatella MA
Blood, 2004-09-09;105(2):830-7.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Enhanced circulating half-life and hematopoietic properties of a human granulocyte colony-stimulating factor/immunoglobulin fusion protein.
Authors: Cox GN, Smith DJ, Carlson SJ, Bendele AM, Chlipala EA, Doherty DH
Exp. Hematol., 2004-05-01;32(5):441-9.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot -
Toxoplasma gondii induces granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor secretion by human fibroblasts: implications for neutrophil apoptosis.
Authors: Channon JY, Miselis KA, Minns LA, Dutta C, Kasper LH
Infect. Immun., 2002-11-01;70(11):6048-57.
Species: Human
Sample Types:
Applications: Neutralization
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