Human Fucosyltransferase 3/5 (FUT3/5) Lumenal Domain Antibody

Catalog #: AF4068 Datasheet

Catalog #	Availability	Size / Price	Qty
AF4068
AF4068-SP

All Fucosyltransferase 3/5 (FUT3/5) Antibodies

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Human Fucosyltransferase 3/5 (FUT3/5) Lumenal Domain Antibody Summary

Species Reactivity

Human

Specificity

Detects human FUT3 and human FUT5 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FUT5
Arg35-Thr374
Accession # Q11128

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # 4950-GT)
Recombinant Human Fucosyltransferase 5/FUT5 (Catalog # 4949-GT)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # 4950-GT), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fucosyltransferase 3/5 (FUT3/5)

FUT5 ( alpha -1, 3-fucosyltransferase 5; also Fuc-TV) is a 43 kDa member of the glycosyltransferase 10 family. It is responsible for the terminal step in the synthesis of Le^x, sialyl‑Le^x, and Le^a antigens. This is accomplished by the transfer of fucose to preexisting LacNAc or sialyl-LacNAc structures. Human FUT5 is 374 amino acids (aa) in length. It is a type II transmembrane Golgi glycoprotein with a C-terminal lumenal domain (aa’s 35-374). The catalytic domain spans aa’s 77-374, while the acceptor-binding motif includes aa’s 120-126. FUT5 is the product of a single exon gene. Thus, there are no splice variants. FUT5 only occurs in primates; there is no rodent ortholog.

Entrez Gene IDs

2525 (Human)

Alternate Names

CD174; FT3B; Fucosyltransferase 3/5; FucT-III; LE; Les