Human Flt-3/Flk-2 Antibody Summary
Asn27-Asn541
Accession # AAA18947
Applications
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Flt‑3/Flk‑2 Monoclonal Antibody (Catalog # MAB8121).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Flt-3/Flk-2 DuoSet ELISA Kit (Catalog # DY812-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Human Flt‑3/Flk‑2 ELISA Standard Curve. Recombinant Human Flt‑3/Flk‑2 protein was serially diluted 2-fold and captured by Mouse Anti-Human Flt‑3/Flk‑2 Monoclonal Antibody (MAB8121) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Flt‑3/Flk‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF812) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
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Detection of Flt‑3/Flk‑2 in THP‑1 cells by Flow Cytometry THP‑1 cells were stained with Goat Anti-Human Flt‑3/Flk‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF812, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Flt-3/Flk-2
The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Mouse Flt-3 has also been cloned and shown to share 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.
Product Datasheets
Citations for Human Flt-3/Flk-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Soluble phosphorylated fms-like tyrosine kinase III. FLT3 protein in patients with acute myeloid leukemia (AML).
Authors: Ravandi F, Jilani I, Estey E, Kantarjian H, Dey A, Aguilar C, Jitkaroon C, Giles F, O'Brien S, Keating M, Albitar M
Leuk. Res., 2006-12-06;31(6):791-7.
Species: Human
Sample Types: Plasma
Applications: Immunoprecipitation -
Inhibition of FLT3 in MLL. Validation of a therapeutic target identified by gene expression based classification.
Authors: Armstrong SA, Kung AL, Mabon ME, Silverman LB, Stam RW, Den Boer ML, Pieters R, Kersey JH, Sallan SE, Fletcher JA, Golub TR, Griffin JD, Korsmeyer SJ
Cancer Cell, 2003-02-01;3(2):173-83.
Species: Human
Sample Types: Whole Cells
Applications: Immunoprecipitation
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