Human Flt-3/Flk-2 Antibody Summary
Asn27-Asn541
Accession # P36888
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Flt-3/Flk-2 Binding to Flt-3 Ligand/FLT3L is Blocked by Flt-3/Flk-2 Antibody. In a functional ELISA binding assay, 0.075-0.75 ug/mL of this antibody will block 50% of the binding of 100 ng/mL of recombinant human rhFlt-3 ligand (308-FK) to immobilized recombinant human Flt-3 (10284-F3) coated at 50 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Flt-3/Flk-2
The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a 130-155 kDa member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF (1). The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) type I membrane protein with a 26 aa signal peptide, a 515 aa extracellular domain (ECD) with 10 potential N-linked glycosylation sites, a 21 aa transmembrane domain and a 431 aa cytoplasmic domain. Within ECD human Flt-3 shares 85% and 82% aa sequence identity with mouse and rat Flt-3, respectively. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist (2).
Product Datasheets
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