Human Fibronectin Protein, CF

Catalog #: 1918-FN
35 Citations 5 Reviews Datasheet

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1918-FN-02M

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All Fibronectin Proteins and Enzymes

Product Details

Citations (35)

FAQs

Reviews (5)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Human Fibronectin Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. The ED₅₀ for this effect is 35.0-420 ng/mL.

Source

Human plasma-derived Fibronectin protein
The human plasma used for the isolation of this product was certified by the supplier to be HIV-1 and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.

Product Datasheets

1918-FN

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1918-FN

Formulation	Supplied as a 0.2 μm filtered solution in Tris-HCl, NaCl and Urea.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, 2 to 8 °C as supplied. 3 months, 2 to 8 °C under sterile conditions after opening. Avoid vortexing and excessive agitation.

Scientific Data

Bioactivity

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Human Fibronectin (Catalog # 1918-FN) supports the adhesion of B16‑F1 mouse melanoma cells. The ED₅₀ for this effect is 35.0-420 ng/mL.

Background: Fibronectin

Fibronectin (Fn) is a large modular glycoprotein that is found as a polymeric fibrillar network in the extracellular matirx (ECM) and as soluble disulfide-linked dimeric protomers in plasma and other body fluids. The protein subunit is made up of three types of homologous structural repeats termed Fn type I, type II, and type III repeats. Multiple isoforms of the protein formed by alternative splicing at numerous sites, resulting in insertions of extra type III domains (EDA and ECB) or parts of the variable type III connecting segment (V/IIICS), have been identified. Fibronectin is a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, and many integrin receptors. It is involved in multiple cellular processes including cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling. Fibronectin functions are mediated by the insoluble polymeric fibrils in the ECM. Conversion of the non-functional soluble Fibronectin to Fibronectin fibrils in the ECM is tightly regulated. The process is initiated by binding of Fibronectin to cell surface integrins, resulting in conformational changes and exposure of cryptic epitopes necessary for polymerization (1, 2).

References

Johansson, S. et al. (1997) Frontiers in Bioscience 2:126.
Geiger, B. et al. (2001) Nature Reviews Molecular Cell Biology 2:793.

Entrez Gene IDs

2335 (Human); 280794 (Bovine)

Alternate Names

CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF

Related Research Areas

Citations for Human Fibronectin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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Chronic Glaucoma Induced in Rats by a Single Injection of Fibronectin-Loaded PLGA Microspheres: IOP-Dependent and IOP-Independent Neurodegeneration
Authors: Munuera, I;Aragon-Navas, A;Villacampa, P;Gonzalez-Cela, MA;Subías, M;Pablo, LE;Garcia-Feijoo, J;Herrero-Vanrell, R;Garcia-Martin, E;Bravo-Osuna, I;Rodrigo, MJ;
International journal of molecular sciences
Species: Rat
Sample Types: In Vivo
Applications: In vivo assay
A distal lung organoid model to study interstitial lung disease, viral infection and human lung development
Authors: Matkovic Leko, I;Schneider, RT;Thimraj, TA;Schrode, N;Beitler, D;Liu, HY;Beaumont, K;Chen, YW;Snoeck, HW;
Nature protocols
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells
Authors: F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber
bioRxiv : the preprint server for biology, 2023-02-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Neuropilin 1 and its inhibitory ligand mini-tryptophanyl-tRNA synthetase inversely regulate VE-cadherin turnover and vascular permeability
Authors: N Gioelli, LJ Neilson, N Wei, G Villari, W Chen, B Kuhle, M Ehling, F Maione, S Willox, S Brundu, D Avanzato, G Koulouras, M Mazzone, E Giraudo, XL Yang, D Valdembri, S Zanivan, G Serini
Nature Communications, 2022-07-20;13(1):4188.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
A Step-by-Step Refined Strategy for Highly Efficient Generation of Neural Progenitors and Motor Neurons from Human Pluripotent Stem Cells
Authors: J Ren, C Li, M Zhang, H Wang, Y Xie, Y Tang
Cells, 2021-11-09;10(11):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Adipocytes disrupt the translational programme of acute lymphoblastic leukaemia to favour tumour survival and persistence
Authors: Q Heydt, C Xintaropou, A Clear, M Austin, I Pislariu, F Miraki-Mou, P Cutillas, K Korfi, M Calaminici, W Cawthorn, K Suchacki, A Nagano, JG Gribben, M Smith, JD Cavenagh, H Oakervee, A Castleton, D Taussig, B Peck, A Wilczynska, L McNaughton, D Bonnet, F Mardakheh, B Patel
Nature Communications, 2021-09-17;12(1):5507.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
Artificial Protein Coronas Enable Controlled Interaction with Corneal Epithelial Cells: New Opportunities for Ocular Drug Delivery
Authors: C Astarita, S Palchetti, M Massaro-Gi, M Di Domenic, F Petrillo, S Boffo, G Caracciolo, A Giordano
Pharmaceutics, 2021-06-12;13(6):.
Species: N/A
Sample Types: Small Molecule
Applications: Bioassay
Induction of cyclophilin A by influenza A virus infection facilitates group A Streptococcus coinfection
Authors: X Bai, W Yang, X Luan, H Li, H Li, D Tian, W Fan, J Li, B Wang, W Liu, L Sun
Cell Reports, 2021-05-18;35(7):109159.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
The in vitro multilineage differentiation and maturation of lung and airway cells from human pluripotent stem cell-derived lung progenitors in 3D
Authors: AL Rodrigues, HY Liu, YW Chen, M Porotto, A Moscona, HW Snoeck
Nature Protocols, 2021-03-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions
Authors: S Bhat, P Viswanatha, S Chandanala, SJ Prasanna, RN Seetharam
Scientific Reports, 2021-02-09;11(1):3403.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Identification of drivers of breast cancer invasion by secretome analysis: insight into CTGF signaling
Authors: JW Hellinger, F Schömel, JV Buse, C Lenz, G Bauerschmi, G Emons, C Gründker
Sci Rep, 2020-10-21;10(1):17889.
Species: Human
Sample Types: Organoid
Applications: Bioassay

Targeted gene correction of human hematopoietic stem cells for the treatment of Wiskott -ï¿½Aldrich Syndrome
Authors: R Rai, M Romito, E Rivers, G Turchiano, G Blattner, W Vetharoy, D Ladon, G Andrieux, F Zhang, M Zinicola, D Leon-Rico, G Santilli, AJ Thrasher, A Cavazza
Nat Commun, 2020-08-12;11(1):4034.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Aerobic and resistance training enhances endothelial progenitor cell function via upregulation of caveolin-1 in mice with type 2 diabetes
Authors: L Zhai, Y Liu, W Zhao, Q Chen, T Guo, W Wei, Z Luo, Y Huang, C Ma, F Huang, X Dai
Stem Cell Res Ther, 2020-01-03;11(1):10.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells
Authors: NV Ayala-Nune, G Follain, F Delalande, A Hirschler, E Partiot, GL Hale, BC Bollweg, J Roels, M Chazal, F Bakoa, M Carocci, S Bourdoulou, O Faklaris, SR Zaki, A Eckly, B Uring-Lamb, F Doussau, S Cianferani, C Carapito, FMJ Jacobs, N Jouvenet, JG Goetz, R Gaudin
Nat Commun, 2019-09-27;10(1):4430.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tyroxine Hydroxylase-Positive Neuronal Cell Population is Increased by Temporal Dioxin Exposure at Early Stage of Differentiation from Human Embryonic Stem Cells
Authors: S Nath Sarma, R Nagano, S Ohsako
Int J Mol Sci, 2019-05-31;20(11):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells
Authors: D Karpova, MP Rettig, J Ritchey, D Cancilla, S Christ, L Gehrs, E Chendamara, MO Evbuomwan, M Holt, J Zhang, G Abou-Ezzi, H Celik, E Wiercinska, W Yang, F Gao, LG Eissenberg, RF Heier, SD Arnett, MJ Meyers, MJ Prinsen, DW Griggs, A Trumpp, PG Ruminski, DM Morrow, HB Bonig, DC Link, JF DiPersio
J. Clin. Invest., 2019-05-14;129(7):2745-2759.
Species: Human
Sample Types: Protein
Applications: Bioassay

DLC2 operates as a tumor suppressor gene in breast cancer via the RhoGTPase pathway
Authors: Z Yang, H Chen, M Shu, Y Zhang, L Xue, Y Lin
Oncol Lett, 2018-12-28;17(2):2107-2116.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Angiopoietin-4-dependent venous maturation and fluid drainage in the peripheral retina
Authors: H Elamaa, M Kihlström, E Kapiainen, M Kaakinen, I Miinalaine, S Ragauskas, M Cerrada-Gi, S Mering, M Nätynki, L Eklund
Elife, 2018-11-16;7(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Effects of Brain-Derived Neurotrophic Factor on MicroRNA Expression Profile in Human Endothelial Progenitor Cells
Authors: T He, R Sun, Y Li, ZS Katusic
Cell Transplant, 2018-06-04;0(0):9636897187616.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zika virus infected primary microglia impairs NPCs proliferation and differentiation
Authors: J Wang, J Liu, R Zhou, X Ding, Q Zhang, C Zhang, L Li
Biochem. Biophys. Res. Commun., 2018-02-15;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Wogonin Suppresses the Activity of Matrix Metalloproteinase-9 and Inhibits Migration and Invasion in Human Hepatocellular Carcinoma
Authors: M Hong, H Cheng, L Song, W Wang, Q Wang, D Xu, W Xing
Molecules, 2018-02-11;23(2):.
Applications: Bioassay

Oncogenic miR-19a and miR-19b co-regulate tumor suppressor MTUS1 to promote cell proliferation and migration in lung cancer
Authors: Y Gu, S Liu, X Zhang, G Chen, H Liang, M Yu, Z Liao, Y Zhou, CY Zhang, T Wang, C Wang, J Zhang, X Chen
Protein Cell, 2017-03-31;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Innate CD8??(+) lymphocytes enhance anti-CD40 antibody-mediated colitis in mice
Authors: AA Kumar, AG Delgado, MB Piazuelo, L Van Kaer, D Olivares-V
Immun Inflamm Dis, 2017-02-20;5(2):109-123.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Senescent peritoneal mesothelium creates a niche for ovarian cancer metastases
Cell Death Dis, 2016-12-29;7(12):e2565.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

PPFIA1 drives active ?5?1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.
Authors: Giulia Mana, Fabiana Clapero, Emiliano Panieri, Valentina Panero, Ralph T Böttcher, Hui-Yuan Tseng, Federico Saltarin, Elena Astanina, Katarzyna I Wolanska, Mark R Morgan, Martin J Humphries, Massimo M Santoro, Guido Serini, Donatella Valdembri
Nature Communications, 2016-11-23;0(0):2041-1723.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

alpha6beta1- and alphaV-integrins are required for long-term self-renewal of murine embryonic stem cells in the absence of LIF.
Authors: Cattavarayane S, Palovuori R, Tanjore Ramanathan J, Manninen A
BMC Cell Biol, 2015-02-27;16(0):3.
Species: Mouse
Sample Types: Whole Cells
Applications: Adhesion Assay, Bioassay

CRK proteins selectively regulate T cell migration into inflamed tissues.
Authors: Huang, Yanping, Clarke, Fiona, Karimi, Mobin, Roy, Nathan H, Williamson, Edward K, Okumura, Mariko, Mochizuki, Kazuhiro, Chen, Emily J, Park, Tae-Ju, Debes, Gudrun F, Zhang, Yi, Curran, Tom, Kambayashi, Taku, Burkhardt, Janis K
J Clin Invest, 2015-01-26;125(3):1019-32.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

miR-193a-3p functions as a tumor suppressor in lung cancer by down-regulating ERBB4.
Authors: Liang H, Liu M, Yan X, Zhou Y, Wang W, Wang X, Fu Z, Wang N, Zhang S, Wang Y, Zen K, Zhang C, Hou D, Li J, Chen X
J Biol Chem, 2014-11-12;290(2):926-40.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

miR-203 suppresses the proliferation and migration and promotes the apoptosis of lung cancer cells by targeting SRC.
Authors: Wang, Nan, Liang, Hongwei, Zhou, Yong, Wang, Chen, Zhang, Suyang, Pan, Yi, Wang, Yanbo, Yan, Xin, Zhang, Junfeng, Zhang, Chen-Yu, Zen, Ke, Li, Donghai, Chen, Xi
PLoS ONE, 2014-08-20;9(8):e105570.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

3D niche microarrays for systems-level analyses of cell fate.
Authors: Ranga A, Gobaa S, Okawa Y, Mosiewicz K, Negro A, Lutolf M
Nat Commun, 2014-07-14;5(0):4324.
Applications: Bioassay

Tivantinib (ARQ197) displays cytotoxic activity that is independent of its ability to bind MET.
Authors: Basilico C, Pennacchietti S, Vigna E, Chiriaco C, Arena S, Bardelli A, Valdembri D, Serini G, Michieli P
Clin Cancer Res, 2013-03-26;19(9):2381-92.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
Authors: Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
Int. J. Cancer, 2010-07-15;127(2):332-44.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Dithiolethione compounds inhibit Akt signaling in human breast and lung cancer cells by increasing PP2A activity.
Authors: Switzer CH, Ridnour LA, Cheng RY, Sparatore A, Del Soldato P, Moody TW, Vitek MP, Roberts DD, Wink DA
Oncogene, 2009-08-24;28(43):3837-46.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Inhibition of endothelial progenitor cell differentiation by VEGI.
Authors: Tian F, Liang PH, Li LY
Blood, 2009-03-27;113(21):5352-60.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Lrp6 is required for convergent extension during Xenopus gastrulation.
Authors: Tahinci E, Thorne CA, Franklin JL, Salic A, Christian KM, Lee LA, Coffey RJ, Lee E
Development, 2007-11-01;134(22):4095-106.
Species: Xenopus
Sample Types: Whole Cells
Applications: Bioassay

FAQs

What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?
- Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application.
What is the molecular weight of Human Fibronectin Protein, CF (Catalog # 1918-FN)?
- This Human Fibronectin Protein is purified from human plasma. There are multiple isoforms of fibronectin that vary in molecular weight. Due to the varied weight of the different isoforms, the MW of purified fibronectin can run between 180-285 kDa in an SDS-PAGE gel under reducing conditions.
The storage recommendation listed on the CoA and datasheet for Human Fibronectin Protein, CF (catalog # 1918-FN) says to store in a manual defrost freezer, yet store at 2 to 8 °C. Can 1918-FN be frozen?
- Catalog #1918-FN should be stored exclusively at 2 to 8 °C and never frozen. We are working to correct the language about the manual defrost freezer.