Human Fibronectin APC-conjugated Antibody

Catalog #: IC1918A Datasheet

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IC1918A

Detection of Fibronectin in WS‑1 Human Cell Line by Flow Cytometry.

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All Fibronectin Antibodies

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Human Fibronectin APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fibronectin in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # P1H11

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human plasma-derived Fibronectin

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Fibronectin antibody in WS‑1 Human Cell Line antibody by Flow Cytometry.

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Detection of Fibronectin in WS‑1 Human Cell Line by Flow Cytometry. WS-1 human fetal skin fibroblast cell line was stained with Mouse Anti-Human Fibronectin APC-conjugated Monoclonal Antibody (Catalog # IC1918A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fibronectin

Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.

Entrez Gene IDs

2335 (Human); 280794 (Bovine)

Alternate Names

CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF