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Human FGFR2 (IIIb) Antibody

Catalog #: MAB665
4 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB665
MAB665-SP
FGF R2 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGFR2 (IIIb) Antibody Summary

Species Reactivity
Human
Specificity
Reacts with multiple isoforms of human FGF R2 although it shows a marked preference for human FGF R2 (IIIb) isoforms in some assays as described below. In direct ELISAs, displays a three-fold preference for FGF R2 (IIIb) isoforms over (IIIc) isoforms. Shows approximately 50% cross-reactivity with recombinant mouse (rm) FGF R2 isoforms and no cross-reactivity with any isoforms of recombinant human (rh) FGF R1 or rhFGF R3. In Western blots, shows approximately 25% cross-reactivity with rhFGF R2 (IIIc) isoforms, 100% cross-reactivity with all isoforms of rmFGF R2 and no cross-reactivity with any isoforms of rhFGF RI, rhFGF R3, or rhFGF R4. Neutralizes the bioactivity of rhFGF R2 (IIIb) isoforms.
Source
Monoclonal Mouse IgG1 Clone # 98707
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human FGF R2 isoforms and S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF R2 isoforms
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human FGF R2 beta (IIIb) Fc Chimera (Catalog # 665-FR)
Neutralization
Measured by its ability to neutralize FGF R2 alpha -mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 6 ng/mL Recombinant Human FGF R2 alpha (IIIb) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization FGF R2 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody. View Larger

FGF R2 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody. Recombinant Human FGF R2a (IIIb) Fc Chimera (Catalog # 663-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R2a (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R2 (IIIb) Monoclonal Antibody (Catalog # MAB665). The ND50 is typically 2-8 µg/mL in the presence of heparin (10 µg/mL).

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR2

FGF R2 is a transmembrane tyrosine kinase that functions as a receptor for several FGF family proteins. The extracellular domain includes three ( alpha isoforms) or two ( beta isoforms) Ig-like domains. Alternative exon usage results in IIIb or IIIc isoforms that differ in their membrane proximal Ig-like domain.

Long Name
Fibroblast Growth Factor Receptor 2
Entrez Gene IDs
2263 (Human); 14183 (Mouse)
Alternate Names
BBDS; BEK; BFR-1; CD332 antigen; CD332; CEK3; CFD1; craniofacial dysostosis 1; EC 2.7.10; EC 2.7.10.1; ECT1; FGF R2; FGFR2; fibroblast growth factor receptor 2; FLJ98662; Jackson-Weiss syndrome; JWS; Keratinocyte growth factor receptorreceptor like 14; KGFR; KSAM; K-sam; soluble FGFR4 variant 4; TK14; TK25

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Citations for Human FGFR2 (IIIb) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. FGFR-mediated ERK1/2 signaling contributes to mesendoderm and definitive endoderm formation in vitro
    Authors: Hwee Hui Lau, Nur Shabrina Amirruddin, Larry Sai Weng Loo, Jun Wei Chan, Elhadi Iich, Vidhya Gomathi Krishnan et al.
    iScience
  2. Effect of tumour-cell-derived or recombinant keratinocyte growth factor (KGF) on proliferation and radioresponse of human epithelial tumour cells (HNSCC) and normal keratinocytes in vitro
    Authors: Andrea Hille, Susanne Grüger, Hans Christiansen, Hendrik A. Wolff, Beate Volkmer, Jörg Lehmann et al.
    Radiation and Environmental Biophysics
  3. Alveolar cell fate selection and lifelong maintenance of AT2 cells by FGF signaling
    Authors: DG Brownfield, AD de Arce, E Ghelfi, A Gillich, TJ Desai, MA Krasnow
    Nature Communications, 2022-11-21;13(1):7137.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Translocation of fibroblast growth factor-10 and its receptor into nuclei of human urothelial cells.
    Authors: Kosman J, Carmean N, Leaf EM, Dyamenahalli K, Bassuk JA
    J. Cell. Biochem., 2007-10-15;102(3):769-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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