Human FGF basic/FGF2/bFGF Quantikine ELISA Kit Summary
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human FGF basic in this assay. No medical histories were available for the donors used in this study.Sample Type | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
Serum | ND | 0 | ND |
EDTA plasma | 13.5 | 10 | ND-14.6 |
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 28.8 | 104 | 234 | 32.8 | 108 | 229 |
Standard Deviation | 2.8 | 3.2 | 7.1 | 3 | 8 | 17.3 |
CV% | 9.7 | 3.1 | 3 | 9.1 | 7.4 | 7.6 |
Recovery
The recovery of FGF basic spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 108 | 94-119 |
EDTA Plasma (n=5) | 103 | 90-124 |
Serum (n=5) | 106 | 86-124 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: FGF basic/FGF2/bFGF
FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human FGF basic/FGF2/bFGF Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Amelioration of Photoreceptor Degeneration by Intravitreal Transplantation of Retinal Progenitor Cells in Rats
Authors: Yang, J;Lewis, GP;Hsiang, CH;Menges, S;Luna, G;Cho, W;Turovets, N;Fisher, SK;Klassen, H;
International journal of molecular sciences
Species: Human
Sample Types: Cell Culture Supernates
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Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction
Authors: Zhang, J;Li, J;Qu, X;Liu, Y;Sun, L;Harada, A;Hua, Y;Sougawa, N;Tabata, A;Liu, L;Miyagawa, S;
Bioactive materials
Species: Human
Sample Types: Cell Culture Supernates
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L-PRF Secretome from Both Smokers/Nonsmokers Stimulates Angiogenesis and Osteoblast Differentiation In Vitro
Authors: Ríos, S;González, LG;Saez, CG;Smith, PC;Escobar, LM;Martínez, CE;
Biomedicines
Species: Human
Sample Types: Serum
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A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and Subvariants
Authors: Scovino, AM;Dahab, EC;Diniz-Lima, I;de Senna Silveira, E;Barroso, SPC;Cardoso, KM;Nico, D;Makhoul, GJ;da Silva-Junior, EB;Freire-de-Lima, CG;Freire-de-Lima, L;Fonseca, LMD;Valente, N;Nacife, V;Machado, A;Araújo, M;Vieira, GF;Pauvolid-Corrêa, A;Siqueira, M;Morrot, A;
Microorganisms
Species: Human
Sample Types: Cell Culture Supernates
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Fluoride-Incorporated Apatite Coating on Collagen Sponge as a Carrier for Basic Fibroblast Growth Factor
Authors: Pal, A;Oyane, A;Nakamura, M;Koga, K;Nishida, E;Miyaji, H;
International journal of molecular sciences
Species: Human
Sample Types: Plasma
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Fibroblasts are the most suitable cell source for regenerative medicine due to their high intracellular fibroblast growth factor 2 content
Authors: Yanagihara, M;Matsuno, Y;Ueno, K;Kurazumi, H;Suzuki, R;Tanaka, T;Hamano, K;
Biochemistry and biophysics reports
Species: Human
Sample Types: Cell Culture Supernates
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Angiogenic and Microvascular Status Alterations after Endovascular Revascularization of Lower Limb Arteries among Patients with Diabetic Foot Syndrome: A Prospective 12-Month Follow-Up Study
Authors: Schönborn, M;Gregorczyk-Maga, I;Batko, K;Maga, M;Bogucka, K;Gawlik, K;Pawlica-Gosiewska, D;Maga, P;
Journal of clinical medicine
Species: Human
Sample Types: Plasma
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rAAV TGF-beta and FGF-2 Overexpression via pNaSS-Grafted PCL Films Stimulates the Reparative Activities of Human ACL Fibroblasts
Authors: Amini, M;Venkatesan, JK;Nguyen, TN;Liu, W;Leroux, A;Madry, H;Migonney, V;Cucchiarini, M;
International journal of molecular sciences
Species: Human
Sample Types: Cell Culture Supernates
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Circulating Angiogenic Factors and Ischemic Diabetic Foot Syndrome Advancement-A Pilot Study
Authors: Schönborn, M;Gregorczyk-Maga, I;Batko, K;Bogucka, K;Maga, M;P?otek, A;Pasieka, P;S?owi?ska-Solnica, K;Maga, P;
Biomedicines
Species: Human
Sample Types: Plasma
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Stromal Vascular Fraction Cells from Individuals Who Have Previously Undergone Radiotherapy Retain Their Pro-Wound Healing Properties
Authors: LV Trevor, K Riches-Sum, AL Mahajan, MJ Thornton
Journal of Clinical Medicine, 2023-03-04;12(5):.
Species: Human
Sample Types: Cell Culture Supernates
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Abeta42 treatment of the brain side reduced the level of flotillin from endothelial cells on the blood side via FGF-2 signaling in a blood-brain barrier model
Authors: T Nakamura, T Hashita, Y Chen, Y Gao, Y Sun, S Islam, H Sato, Y Shibuya, K Zou, T Matsunaga, M Michikawa
Molecular Brain, 2023-01-26;16(1):15.
Species: Human
Sample Types: Cell Culture Supernates
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A comparative study of pathogen inactivation technologies in human platelet lysate and its optimal efficiency in human placenta-derived stem cells culture
Authors: E Razani, HR Aghayan, S Alavi-Mogh, F Yari, A Gharehbagh, Z Sharifi
Journal of virological methods, 2022-01-29;0(0):114478.
Species: Human
Sample Types: Cell Lysates
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Matrix stiffness modulates hepatic stellate cell activation into tumor-promoting myofibroblasts via E2F3-dependent signaling and regulates malignant progression
Authors: Z Liu, H Mo, R Liu, Y Niu, T Chen, Q Xu, K Tu, N Yang
Cell Death & Disease, 2021-12-06;12(12):1134.
Species: Human
Sample Types: Cell Culture Supernates
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Diphenylarsinic acid induced activation of MAP kinases, transcription factors, and oxidative stress-responsive factors and hypersecretion of cytokines in cultured normal human cerebellar astrocytes
Authors: S Sasaki, T Negishi, T Tsuzuki, K Yukawa
Neurotoxicology, 2021-12-06;88(0):196-207.
Species: Human
Sample Types: Cell Culture Supernates
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Circulating fibroblast growth factor-2 precipitates HIV nephropathy in mice
Authors: JR Das, M Jerebtsova, P Tang, J Li, J Yu, PE Ray
Disease Models & Mechanisms, 2021-07-26;14(7):.
Species: Mouse
Sample Types: Plasma
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Defined MSC exosome with high yield and purity to improve regenerative activity
Authors: JY Kim, WK Rhim, YI Yoo, DS Kim, KW Ko, Y Heo, CG Park, DK Han
Journal of tissue engineering, 2021-04-20;12(0):2041731421100.
Species: Human
Sample Types: Cell Lysates
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In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel
Authors: N Casagrande, C Borghese, F Agostini, C Durante, M Mazzucato, A Colombatti, D Aldinucci
International Journal of Molecular Sciences, 2021-03-16;22(6):.
Species: Human
Sample Types: Plasma
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Circulating Fibroblast Growth Factor-2 precipitates HIV-nephropathy in mice
Authors: JR Das, M Jerebtsova, P Tang, J Li, J Yu, PE Ray
Disease Models & Mechanisms, 2021-01-01;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Therapeutic paradigm of dual targeting VEGF and PDGF for effectively treating FGF-2 off-target tumors
Authors: K Hosaka, Y Yang, T Seki, Q Du, X Jing, X He, J Wu, Y Zhang, H Morikawa, M Nakamura, M Scherzer, X Sun, Y Xu, T Cheng, X Li, X Liu, Q Li, Y Liu, A Hong, Y Chen, Y Cao
Nat Commun, 2020-07-24;11(1):3704.
Species: Mouse
Sample Types: Cell Culture Supernates
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Repeated Freezing Procedures Preserve Structural and Functional Properties of Amniotic Membrane for Application in Ophthalmology
Authors: O Pogozhykh, N Hofmann, O Gryshkov, C von Kaisen, M Mueller, B Glasmacher, D Pogozhykh, M Börgel, R Blasczyk, C Figueiredo
Int J Mol Sci, 2020-06-04;21(11):.
Species: Human
Sample Types: Tissue Homogenates
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PEG grafted chitosan scaffold for dual growth factor delivery for enhanced wound healing
Authors: A Vijayan, S A, GSV Kumar
Sci Rep, 2019-12-16;9(1):19165.
Species: Human
Sample Types: Hydrogel Supernates
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Circulating pro- and anti-angiogenic factors in multi-stage liver disease and hepatocellular carcinoma progression
Authors: YY Joo, JW Jang, SW Lee, SH Yoo, JH Kwon, SW Nam, SH Bae, JY Choi, SK Yoon
Sci Rep, 2019-06-24;9(1):9137.
Species: Human
Sample Types: Serum
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Impact of HOXB7 overexpression on human adipose-derived mesenchymal progenitors
Authors: EM Foppiani, O Candini, I Mastrolia, A Murgia, G Grisendi, AV Samarelli, G Boscaini, L Pacchioni, M Pinelli, G De Santis, EM Horwitz, E Veronesi, M Dominici
Stem Cell Res Ther, 2019-03-19;10(1):101.
Species: Human
Sample Types: Cell Culture Supernates
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FGF signal is not required for hepatoblast differentiation of human iPS cells
Authors: Y Toba, A Kiso, S Nakamae, F Sakurai, K Takayama, H Mizuguchi
Sci Rep, 2019-03-06;9(1):3713.
Species: Human
Sample Types: Cell Culture Supernates
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Collagen/Gelatin Sponges (CGSs) Provide Both Protection and Release of bFGF: An In Vitro Study
Authors: MC Munisso, N Morimoto, SC Notodihard, T Mitsui, N Kakudo, K Kusumoto
Biomed Res Int, 2019-02-19;2019(0):4016351.
Species: Human
Sample Types: Sponge Fluid
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Stromal fibroblast growth factor 2 reduces the efficacy of bromodomain inhibitors in uveal melanoma
Authors: V Chua, M Orloff, JL Teh, T Sugase, C Liao, TJ Purwin, BQ Lam, M Terai, G Ambrosini, RD Carvajal, G Schwartz, T Sato, AE Aplin
EMBO Mol Med, 2019-02-01;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Ginsenoside Rg1 Accelerates Paracrine Activity and Adipogenic Differentiation of Human Breast Adipose-Derived Stem Cells in a Dose-Dependent Manner In Vitro
Authors: ZJ Liang, X Lu, DD Zhu, XL Yi, FX Wu, N He, C Tang, CY Wei, HM Li
Cell Transplant, 2019-01-24;0(0):9636897198256.
Species: Human
Sample Types: Cell Culture Supernates
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1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures
Authors: F Agostini, M Ruzza, D Corpillo, L Biondi, E Acquadro, B Canepa, A Viale, M Battiston, F Serra, S Aime, M Mazzucato
PLoS ONE, 2018-09-06;13(9):e0203048.
Species: Human
Sample Types: Cell Culture Supernates
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NF-?B activation is an early event of changes in gene regulation for acquiring drug resistance in human adenocarcinoma PC-9 cells
Authors: M Fukuoka, K Yoshioka, H Hohjoh
PLoS ONE, 2018-08-03;13(8):e0201796.
Species: Human
Sample Types: Cell Culture Supernates
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Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches
Authors: C Saury, A Lardenois, C Schleder, I Leroux, B Lieubeau, L David, M Charrier, L Guével, S Viau, B Delorme, K Rouger
Stem Cell Res Ther, 2018-05-02;9(1):128.
Species: Human
Sample Types: Cell Culture Supernates
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Kaempferol targeting on the fibroblast growth factor receptor 3-ribosomal S6 kinase 2 signaling axis prevents the development of rheumatoid arthritis
Authors: CJ Lee, SJ Moon, JH Jeong, S Lee, MH Lee, SM Yoo, HS Lee, HC Kang, JY Lee, WS Lee, HJ Lee, EK Kim, JY Jhun, ML Cho, JK Min, YY Cho
Cell Death Dis, 2018-03-14;9(3):401.
Species: Human
Sample Types: Synovial Fluid
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Enhanced rapamycin delivery to hemangiomas by lipid polymer nanoparticles coupled with anti-VEGFR antibody
Authors: H Li, Y Teng, X Xu, J Liu
Int. J. Mol. Med., 2018-02-28;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Comparative analyses of industrial-scale human platelet lysate preparations.
Authors: Jan Pierce, Eric Benedetti, Amber Preslar, Pam Jacobson, Ping Jin, David F Stroncek, Jo-Anna Reems
Transfusion, 2017-10-08;0(0):1537-2995.
Species: Human
Sample Types: Plasma
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Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy
Authors: K Nagaishi, Y Mizue, T Chikenji, M Otani, M Nakano, Y Saijo, H Tsuchida, S Ishioka, A Nishikawa, T Saito, M Fujimiya
Sci Rep, 2017-08-16;7(1):8484.
Species: Human
Sample Types: Cell Culture Supernates
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Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
Authors: Y Zhang, BJ Morgan, R Smith, CR Fellows, C Thornton, M Snow, LW Francis, IM Khan
Sci Rep, 2017-06-16;7(1):3699.
Species: Human
Sample Types: Serum
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Prognostic impact of blood and urinary angiogenic factor levels at diagnosis and during treatment in patients with osteosarcoma: a prospective study
Authors: MD Tabone, L Brugières, S Piperno-Ne, MA Selva, P Marec-Béra, H Pacquement, C Lervat, N Corradini, JC Gentet, R Couderc, A Chevance, C Mahier-Ait, N Entz-Werle, JY Blay, MC Le Deley
BMC Cancer, 2017-06-15;17(1):419.
Species: Human
Sample Types: Serum
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The inhibition of FGF receptor 1 activity mediates sorafenib antiproliferative effects in human malignant pleural mesothelioma tumor-initiating cells
Authors: A Pattarozzi, E Carra, RE Favoni, R Würth, D Marubbi, RA Filiberti, L Mutti, T Florio, F Barbieri, A Daga
Stem Cell Res Ther, 2017-05-25;8(1):119.
Species: Human
Sample Types: Cell Culture Supernates
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The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro
Authors: M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori
J Transl Med, 2017-05-01;15(1):90.
Species: Human
Sample Types: Cell Lysates
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Evaluation of autologous skeletal muscle-derived factors for regenerative medicine applications
Authors: M Yoshikawa, T Nakasa, M Ishikawa, N Adachi, M Ochi
Bone Joint Res, 2017-05-01;6(5):277-283.
Species: Human
Sample Types: Tissue Homogenates
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Comparison of fibrin glue and Vicryl sutures in conjunctival autografting for pterygium surgery
Authors: X Wang, Y Zhang, L Zhou, R Wei, L Dong
Mol. Vis., 2017-04-19;23(0):275-285.
Species: Human
Sample Types: Tears
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PH20 is not expressed in murine CNS and oligodendrocyte precursor cells
Authors: M Marella, J Ouyang, J Zombeck, C Zhao, L Huang, RJ Connor, KB Phan, MC Jorge, MA Printz, RD Paladini, AB Gelb, Z Huang, GI Frost, BJ Sugarman, L Steinman, G Wei, HM Shepard, DC Maneval, PJ Lapinskas
Ann Clin Transl Neurol, 2017-02-23;4(3):191-211.
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Adipogenic impairment of adipose tissue-derived mesenchymal stem cells in subjects with metabolic syndrome: possible protective role of FGF2
Authors: Wilfredo Oliva-Oliv
J. Clin. Endocrinol. Metab, 2017-02-01;0(0):jc20162256.
Species: Human
Sample Types: Cell Culture Supernates
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Impact of Hyperglycemia and Low Oxygen Tension on Adipose-Derived Stem Cells Compared with Dermal Fibroblasts and Keratinocytes: Importance for Wound Healing in Type 2 Diabetes
PLoS ONE, 2016-12-19;11(12):e0168058.
Species: Human
Sample Types: Cell Culture Supernates
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Overcoming the bottleneck of platelet lysate supply in large-scale clinical expansion of adipose-derived stem cells: A comparison of fresh versus three types of platelet lysates from outdated buffy coat-derived platelet concentrates
Authors: Anne Fischer-Ni
Cytotherapy, 2016-11-22;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
Sci Rep, 2016-07-04;6(0):29029.
Species: Human
Sample Types: Cell Culture Supernates
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Expression of Heat Shock Protein 27 in Melanoma Metastases Is Associated with Overall Response to Bevacizumab Monotherapy: Analyses of Predictive Markers in a Clinical Phase II Study
PLoS ONE, 2016-05-11;11(5):e0155242.
Species: Human
Sample Types: Serum
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Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved
Authors: D Dauwe, B Pelacho, A Wibowo, AS Walravens, K Verdonck, H Gillijns, E Caluwe, P Pokreisz, N van Gastel, G Carmeliet, M Depypere, F Maes, N Vanden Dri, W Droogne, J Van Cleemp, J Vanhaecke, F Prosper, C Verfaillie, A Luttun, S Janssens
J Am Heart Assoc, 2016-04-18;5(4):.
Species: Human
Sample Types: Cell Culture Supernates
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A promising scaffold with excellent cytocompatibility and pro-angiogenesis action for dental tissue engineering: Strontium-doped calcium polyphosphate
Authors: H Qin, Z Yang, L Li, X Yang, J Liu, X Chen, X Yu
Dent Mater J, 2016-01-01;35(2):241-9.
Species: Human
Sample Types: Cell Culture Supernates
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Breeding Strategy Determines Rupture Incidence in Post-Infarct Healing WARPing Cardiovascular Research.
Authors: Deckx S, Carai P, Bateman J, Heymans S, Papageorgiou A
PLoS ONE, 2015-09-25;10(9):e0139199.
Species: Rat
Sample Types: Cell Culture Supernates
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Mesenchymal progenitors aging highlights a miR-196 switch targeting HOXB7 as master regulator of proliferation and osteogenesis.
Authors: Candini O, Spano C, Murgia A, Grisendi G, Veronesi E, Piccinno M, Ferracin M, Negrini M, Giacobbi F, Bambi F, Horwitz E, Conte P, Paolucci P, Dominici M
Stem Cells, 2015-03-01;33(3):939-50.
Species: Human
Sample Types: Cell Culture Supernates
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In situ normoxia enhances survival and proliferation rate of human adipose tissue-derived stromal cells without increasing the risk of tumourigenesis.
Authors: Choi, Jane Ru, Pingguan-Murphy, Belinda, Wan Abas, Wan Abu, Yong, Kar Wey, Poon, Chi Tat, Noor Azmi, Mat Aden, Omar, Siti Zaw, Chua, Kien Hui, Xu, Feng, Wan Safwani, Wan Kama
PLoS ONE, 2015-01-23;10(1):e0115034.
Species: Human
Sample Types: Cell Culture Supernates
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Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate.
Authors: Chou M, Burnouf T, Wang T
PLoS ONE, 2014-06-19;9(6):e99145.
Species: Human
Sample Types: Cell Culture Supernates
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A novel decoy receptor fusion protein for FGF-2 potently inhibits tumour growth.
Authors: Li D, Wei X, Xie K, Chen K, Li J, Fang J
Br J Cancer, 2014-05-29;111(1):68-77.
Species: Human
Sample Types: Recombinant Protein
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Malondialdehyde mediates oxidized LDL-induced coronary toxicity through the Akt-FGF2 pathway via DNA methylation.
Authors: Yang T, Chen Y, Chang S, Chen C, Chang P, Lu S
J Biomed Sci, 2014-02-03;21(0):11.
Species: Human
Sample Types: Cell Culture Supernates
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Increased pericyte coverage mediated by endothelial-derived fibroblast growth factor-2 and interleukin-6 is a source of smooth muscle-like cells in pulmonary hypertension.
Authors: Ricard N, Tu L, Le Hiress M, Huertas A, Phan C, Thuillet R, Sattler C, Fadel E, Seferian A, Montani D, Dorfmuller P, Humbert M, Guignabert C
Circulation, 2014-01-30;129(15):1586-97.
Species: Human
Sample Types: Cell Culture Supernates
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Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells.
Authors: Iudicone, Paola, Fioravanti, Daniela, Bonanno, Giuseppi, Miceli, Michelin, Lavorino, Claudio, Totta, Pierange, Frati, Luigi, Nuti, Marianna, Pierelli, Luca
J Transl Med, 2014-01-27;12(0):28.
Species: Human
Sample Types: Cell Culture Supernates
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The evolution of cellular deficiency in GATA2 mutation.
Authors: Dickinson R, Milne P, Jardine L, Zandi S, Swierczek S, McGovern N, Cookson S, Ferozepurwalla Z, Langridge A, Pagan S, Gennery A, Heiskanen-Kosma T, Hamalainen S, Seppanen M, Helbert M, Tholouli E, Gambineri E, Reykdal S, Gottfreethsson M, Thaventhiran J, Morris E, Hirschfield G, Richter A, Jolles S, Bacon C, Hambleton S, Haniffa M, Bryceson Y, Allen C, Prchal J, Dick J, Bigley V, Collin M
Blood, 2013-12-17;123(6):863-74.
Species: Human
Sample Types: Serum
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The role of SDF-1-CXCR4/CXCR7 axis in biological behaviors of adipose tissue-derived mesenchymal stem cells in vitro.
Authors: Li Q, Zhang A, Tao C, Li X, Jin P
Biochem Biophys Res Commun, 2013-10-30;441(3):675-80.
Species: Human
Sample Types: Cell Culture Supernates
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Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
BMC Cell Biol, 2013-10-30;14(0):48.
Species: Human
Sample Types: Cell Culture Supernates
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A novel splice variant of calcium and integrin-binding protein 1 mediates protein kinase D2-stimulated tumour growth by regulating angiogenesis.
Authors: Armacki, M, Joodi, G, Nimmagadda, S C, de Kimpe, L, Pusapati, G V, Vandoninck, S, Van Lint, J, Illing, A, Seufferlein, T
Oncogene, 2013-03-18;33(9):1167-80.
Species: Human
Sample Types: Cell Culture Supernates
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Study of the quantitative, functional, cytogenetic, and immunoregulatory properties of bone marrow mesenchymal stem cells in patients with B-cell chronic lymphocytic leukemia.
Authors: Pontikoglou C, Kastrinaki M, Klaus M, Kalpadakis C, Katonis P, Alpantaki K, Pangalis G, Papadaki H
Stem Cells Dev, 2013-02-01;22(9):1329-41.
Species: Human
Sample Types: Cell Culture Supernates
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Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis.
Authors: Ho I, Toh H, Ng W, Teo Y, Guo C, Hui K, Lam P
Stem Cells, 2013-01-01;31(1):146-55.
Species: Human
Sample Types: Tissue Homogenates
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Cytomegalovirus impairs cytotrophoblast-induced lymphangiogenesis and vascular remodeling in an in vivo human placentation model.
Authors: Tabata T, Petitt M, Fang-Hoover J, Rivera J, Nozawa N, Shiboski S, Inoue N, Pereira L
Am J Pathol, 2012-09-07;181(5):1540-59.
Species: Human
Sample Types: Cell Culture Supernates
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Differential expression of cancer associated proteins in breast milk based on age at first full term pregnancy.
Authors: Qin W, Zhang K, Kliethermes B, Ruhlen RL, Browne EP, Arcaro KF, Sauter ER
BMC Cancer, 2012-03-21;12(0):100.
Species: Human
Sample Types: Milk
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Biomarkers for antitumor activity of bevacizumab in gastric cancer models.
Authors: Yamashita-Kashima Y, Fujimoto-Ouchi K, Yorozu K, Kurasawa M, Yanagisawa M, Yasuno H, Mori K
BMC Cancer, 2012-01-25;12(0):37.
Species: Human
Sample Types: Tissue Homogenates
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In vitro vascular tube formation testing as a tool for treatment individualisation in patients with cervical cancer.
Authors: Landt S, Heidecke H, Korlach S, Reuter C, Schwidde I, Barinoff J, Thill M, Sehouli J, Kummel S
Anticancer Res., 2011-08-01;31(8):2609-15.
Species: Human
Sample Types: Serum
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Fibroblast growth factor receptors are components of autocrine signaling networks in head and neck squamous cell carcinoma cells.
Authors: Marshall ME, Hinz TK, Kono SA, Singleton KR, Bichon B, Ware KE, Marek L, Frederick BA, Raben D, Heasley LE
Clin. Cancer Res., 2011-06-14;17(15):5016-25.
Species: Human
Sample Types: Cell Lysates
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Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
ALTEX, 2011-01-01;28(4):305-16.
Species: Human
Sample Types: Cell Lysates
-
Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
Authors: Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
Angiogenesis, 2010-10-16;13(4):337-47.
Species: Human
Sample Types: Cell Culture Supernates
-
Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
Cardiovasc. Res., 2010-07-29;89(1):157-65.
Species: Human
Sample Types: Cell Culture Supernates
-
Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch.
Authors: Ding W, Knox TR, Tschumper RC
Blood, 2010-07-06;116(16):2984-93.
Species: Human
Sample Types: Platelet Poor Plasma
-
Lenalidomide plus prednisone results in durable clinical, histopathologic, and molecular responses in patients with myelofibrosis.
Authors: Quintas-Cardama A, Kantarjian HM, Manshouri T, Thomas D, Cortes J, Ravandi F, Garcia-Manero G, Ferrajoli A, Bueso-Ramos C, Verstovsek S
J. Clin. Oncol., 2009-08-31;27(28):4760-6.
Species: Human
Sample Types: Plasma
-
Tumor-derived pancreatic stellate cells promote pancreatic cancer cell invasion through release of thrombospondin-2.
Authors: Farrow B, Berger DH, Rowley D
J. Surg. Res., 2009-05-03;156(1):155-60.
Species: Human
Sample Types: Cell Culture Supernates
-
A urinary biomarker profile for children with HIV-associated renal diseases.
Authors: Soler-Garcia AA, Rakhmanina NY, Mattison PC, Ray PE
Kidney Int., 2009-04-08;76(2):207-14.
Species: Human
Sample Types: Urine
-
Inhibitory effect of atractylenolide I on angiogenesis in chronic inflammation in vivo and in vitro.
Authors: Wang C, Duan H, He L
Eur. J. Pharmacol., 2009-04-06;612(1):143-52.
Species: Mouse
Sample Types: Air Pouch Exudate
-
Elevated fibroblast growth factor-2 increases tumor necrosis factor-alpha induced endothelial cell death in high glucose.
Authors: Clyne AM, Zhu H, Edelman ER
J. Cell. Physiol., 2008-10-01;217(1):86-92.
Species: Porcine
Sample Types: Whole Cells
-
Observations on the microvasculature of bone defects filled with biodegradable nanoparticulate hydroxyapatite.
Authors: Kilian O, Wenisch S, Karnati S, Baumgart-Vogt E, Hild A, Fuhrmann R, Jonuleit T, Dingeldein E, Schnettler R, Franke RP
Biomaterials, 2008-05-23;29(24):3429-37.
Species: Porcine
Sample Types: Plasma
-
Clinical correlations and prognostic relevance of HGF, VEGF AND FGF expression in Brazilian patients with non-Hodgkin lymphoma.
Authors: Etto L, Lacerda E, Baiocchi O, Silva V, Dalboni M, Alves A, Silva M, Vettore A, Colleoni G
Leuk. Lymphoma, 2008-02-01;49(2):257-64.
Species: Human
Sample Types: Serum
-
Connexin43 knockdown accelerates wound healing but inhibits mesenchymal transition after corneal endothelial injury in vivo.
Authors: Nakano Y, Oyamada M, Dai P, Nakagami T, Kinoshita S, Takamatsu T
Invest. Ophthalmol. Vis. Sci., 2008-01-01;49(1):93-104.
Species: Rat
Sample Types: Aqueous Humor
-
Electronegative LDL impairs vascular endothelial cell integrity in diabetes by disrupting fibroblast growth factor 2 (FGF2) autoregulation.
Authors: Lu J, Jiang W, Yang JH, Chang PY, Walterscheid JP, Chen HH, Marcelli M, Tang D, Lee YT, Liao WS, Yang CY, Chen CH
Diabetes, 2007-10-24;57(1):158-66.
Species: Bovine
Sample Types: Cell Culture Supernates
-
Sca-1(+) Hematopoietic Cell-based Gene Therapy with a Modified FGF-2 Increased Endosteal/Trabecular Bone Formation in Mice.
Authors: Hall SL, Lau KH, Chen ST, Wergedal JE, Srivastava A, Klamut H, Sheng MH, Gridley DS, Mohan S, Baylink DJ
Mol. Ther., 2007-07-17;15(10):1881-9.
Species: Mouse
Sample Types: Serum
-
Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma.
Authors: Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K
Int. J. Cancer, 2007-02-01;120(3):686-93.
Species: Human
Sample Types: Cell Culture Supernates
-
Fibroblast growth factor-2 expression is altered in lambs with increased pulmonary blood flow and pulmonary hypertension.
Authors: Wedgwood S, Devol JM, Grobe A, Benavidez E, Azakie A, Fineman JR, Black SM
Pediatr. Res., 2007-01-01;61(1):32-6.
Species: Ovine
Sample Types: Cell Culture Supernates
-
Angiogenic growth factors in maternal and fetal serum in pregnancies complicated by intrauterine growth restriction.
Authors: Wallner W, Sengenberger R, Strick R, Strissel PL, Meurer B, Beckmann MW, Schlembach D
Clin. Sci., 2007-01-01;112(1):51-7.
Species: Human
Sample Types: Serum
-
A phase I biological and pharmacologic study of the heparanase inhibitor PI-88 in patients with advanced solid tumors.
Authors: Basche M, Gustafson DL, Holden SN, O'Bryant CL, Gore L, Witta S, Schultz MK, Morrow M, Levin A, Creese BR, Kangas M, Roberts K, Nguyen T, Davis K, Addison RS, Moore JC, Eckhardt SG
Clin. Cancer Res., 2006-09-15;12(18):5471-80.
Species: Human
Sample Types: Urine
-
Targeted deletion of CCR2 impairs deep vein thombosis resolution in a mouse model.
Authors: Henke PK, Pearce CG, Moaveni DM, Moore AJ, Lynch EM, Longo C, Varma M, Dewyer NA, Deatrick KB, Upchurch GR, Wakefield TW, Hogaboam C, Kunkel SL
J. Immunol., 2006-09-01;177(5):3388-97.
Species: Mouse
Sample Types: Tissue Homogenates
-
Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
Authors: Herrmann S, Green J, Weber T, Scharfenberger A
Bone, 2006-07-25;39(5):1156-63.
Species: Human
Sample Types: Tissue Homogenates
-
Serum levels of syndecan-1 in B-cell chronic lymphocytic leukemia: correlation with the extent of angiogenesis and disease-progression risk in early disease.
Authors: Molica S, Vitelli G, Mirabelli R, Digiesu G, Giannarelli D, Cuneo A, Ribatti D, Vacca A
Leuk. Lymphoma, 2006-06-01;47(6):1034-40.
Species: Human
Sample Types: Serum
-
Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells.
Authors: Moreno MJ, Ball M, Andrade MF, McDermid A, Stanimirovic DB
Glia, 2006-06-01;53(8):845-57.
Species: Human
Sample Types: Cell Culture Supernates
-
Relation of various plasma growth factor levels in patients with stable angina pectoris and total occlusion of a coronary artery to the degree of coronary collaterals.
Authors: Briguori C, Testa U, Colombo A, Petrucci E, Condorelli G, Airoldi F, Peschle C, Condorelli G
Am. J. Cardiol., 2005-12-20;97(4):472-6.
Species: Human
Sample Types: Serum
-
Repair of articular cartilage defect by intraarticular administration of basic fibroblast growth factor gene, using adeno-associated virus vector.
Authors: Hiraide A, Yokoo N, Xin KQ, Okuda K, Mizukami H, Ozawa K, Saito T
Hum. Gene Ther., 2005-12-01;16(12):1413-21.
Species: Rat
Sample Types: Cell Culture Supernates
-
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
J. Immunol., 2005-11-01;175(9):6177-89.
Species: Human
Sample Types: Cell Culture Supernates
-
Functional and structural remodeling of the myocardial microvasculature in early experimental hypertension.
Authors: Rodriguez-Porcel M, Zhu XY, Chade AR, Amores-Arriaga B, Caplice NM, Ritman EL, Lerman A, Lerman LO
Am. J. Physiol. Heart Circ. Physiol., 2005-10-07;290(3):H978-84.
Species: Porcine
Sample Types: Tissue Homogenates
-
Tumor necrosis factor alpha and adiponectin in bone marrow interstitial fluid from patients with acute myeloid leukemia inhibit normal hematopoiesis.
Authors: Iversen PO, Wiig H
Clin. Cancer Res., 2005-10-01;11(19):6793-9.
Species: Human
Sample Types: Plasma
-
Alpha-tocopheryl succinate inhibits malignant mesothelioma by disrupting the fibroblast growth factor autocrine loop: mechanism and the role of oxidative stress.
Authors: Stapelberg M, Gellert N, Swettenham E, Tomasetti M, Witting PK, Procopio A, Neuzil J
J. Biol. Chem., 2005-05-05;280(27):25369-76.
Species: Human
Sample Types: Cell Culture Supernates
-
A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells.
Authors: Nishishita T, Ouchi K, Zhang X, Inoue M, Inazawa T, Yoshiura K, Kuwabara K, Nakaoka T, Watanabe N, Igura K, Takahashi TA, Yamashita N
Biochem. Biophys. Res. Commun., 2004-12-03;325(1):24-31.
Species: Human
Sample Types: Cell Lysates
-
Transplantation of transduced retinal pigment epithelium in rats.
Authors: Saigo Y, Abe T, Hojo M, Tomita H, Sugano E, Tamai M
Invest. Ophthalmol. Vis. Sci., 2004-06-01;45(6):1996-2004.
Species: Rat
Sample Types: Cell Culture Supernates
-
Intravenous administration of marrow stromal cells (MSCs) increases the expression of growth factors in rat brain after traumatic brain injury.
Authors: Mahmood A, Lu D, Chopp M
J. Neurotrauma, 2004-01-01;21(1):33-9.
Species: Rat
Sample Types: Tissue Homogenates
-
Have urinary levels of the angiogenic factors, basic fibroblast growth factor and vascular endothelial growth factor, a prognostic value in childhood acute lymphoblastic leukaemia?
Authors: Schneider P, Vasse M, Legrand E, Callat MP, Vannier JP
Br. J. Haematol., 2003-07-01;122(1):163-4.
Species: Human
Sample Types: Urine
-
beta-Catenin regulates vascular endothelial growth factor expression in colon cancer.
Authors: Easwaran V, Lee SH, Inge L, Guo L, Goldbeck C, Garrett E, Wiesmann M, Garcia PD, Fuller JH, Chan V, Randazzo F, Gundel R, Warren RS, Escobedo J, Aukerman SL, Taylor RN, Fantl WJ
Cancer Res., 2003-06-15;63(12):3145-53.
Species: Mouse
Sample Types: Serum
-
Low-density lipoprotein in hypercholesterolemic human plasma induces vascular endothelial cell apoptosis by inhibiting fibroblast growth factor 2 transcription.
Authors: Chen CH, Jiang T, Yang JH, Jiang W, Lu J, Marathe GK, Pownall HJ, Ballantyne CM, McIntyre TM, Henry PD, Yang CY
Circulation, 2003-04-14;107(16):2102-8.
Species: Bovine
Sample Types: Cell Lysates
-
Diabetes can alter the signal transduction pathways in the lens of rats.
Authors: Zatechka DS, Kador PF, Garcia-Castineiras S, Lou MF
Diabetes, 2003-04-01;52(4):1014-22.
Species: Rat
Sample Types: Vitreous Humor
-
Sugar-induced modification of fibroblast growth factor 2 reduces its angiogenic activity in vivo.
Authors: Facchiano F, Lentini A, Fogliano V, Mancarella S, Rossi C, Facchiano A, Capogrossi MC
Am. J. Pathol., 2002-08-01;161(2):531-41.
Species: Bovine
Sample Types: Cell Culture Supernates
-
Increase in gap junctional intercellular communication by high molecular weight hyaluronic acid associated with fibroblast growth factor 2 and keratinocyte growth factor production in normal human dermal fibroblasts.
Authors: Park JU, Tsuchiya T
Tissue Eng., 2002-07-01;8(3):419-27.
Species: Human
Sample Types: Cell Culture Supernates
-
High basic fibroblast growth factor levels in nipple aspirate fluid are correlated with breast cancer.
Authors: Hsiung R, Zhu W, Klein G, Qin W, Rosenberg A, Park P, Rosato E, Sauter E
Cancer J, 2002-07-01;8(4):303-10.
Species: Human
Sample Types: Tissue Secretion
-
Retinoscleral control of scleral remodelling in refractive development: a role for endogenous FGF-2?
Authors: Gentle A, { –!áà§S|†, McBrien NA
2002-06-21;18(6):344-8.
Species: Tree Shrew
Sample Types: Tissue Homogenates
-
AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
Authors: Truckenmiller ME, Vawter MP, Zhang P, Conejero-Goldberg C, Dillon-Carter O, Morales N, Cheadle C, Becker KG, Freed WJ
Exp. Neurol., 2002-06-01;175(0):318.
Species: Rat
Sample Types: Cell Culture Supernates
-
Basic fibroblast growth factor in human saliva decreases with aging.
Authors: Westermark A, Pyykko I, Magnusson M, Ishizaki H, Jantti P, van Setten G
Laryngoscope, 2002-05-01;112(5):887-9.
Species: Human
Sample Types: Saliva
-
Circadian variations of transforming growth factor-beta2 and basic fibroblast growth factor in the rabbit aqueous humor.
Authors: Liu JH
Curr. Eye Res., 2002-01-01;24(1):75-80.
Species: Rabbit
Sample Types: Aqueous Humor
-
Cyclic mechanical stretching modulates secretion pattern of growth factors in human tendon fibroblasts.
Authors: Skutek M, van Griensven M, Zeichen J, Brauer N, Bosch U
Eur. J. Appl. Physiol., 2001-11-01;86(1):48-52.
Species: Human
Sample Types: Cell Culture Supernates
-
Angiogenic balance in human melanoma: expression of VEGF, bFGF, IL-8, PDGF and angiostatin in relation to vascular density of xenografts in vivo.
Authors: Westphal JR, Van't Hullenaar R, Peek R, Willems RW, Crickard K, Crickard U, Askaa J, Clemmensen I, Ruiter DJ, De Waal RM
Int. J. Cancer, 2000-06-15;86(6):768-76.
Species: Human
Sample Types: Cell Culture Supernates
-
Intestinal mucosal secretion of basic fibroblast growth factor in patients with ulcerative colitis.
Authors: Thorn M, Raab Y, Larsson A, Gerdin B, Hallgren R
Scand. J. Gastroenterol., 2000-04-01;35(4):408-12.
Species: Human
Sample Types: Tissue Perfusion Fluid
-
Growth factors and proinflammatory cytokines in the renal involvement of POEMS syndrome.
Authors: Soubrier M, Sauron C, Souweine B, Larroche C, Wechsler B, Guillevin L, Piette JC, Rousset H, Deteix P
Am. J. Kidney Dis., 1999-10-01;34(4):633-8.
Species: Human
Sample Types: Serum
-
Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells.
Authors: Gu JW, Adair TH
Am. J. Physiol., 1997-08-01;273(2):H628-33.
Species: Canine
Sample Types: Cell Culture Supernates
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