Human FGF basic/FGF2/bFGF Biotinylated Antibody

Catalog #: BAM233
1 Citation 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
BAM233

All FGF basic/FGF2/bFGF Antibodies

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF basic/FGF2/bFGF Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human FGF basic/FGF2/bFGF in ELISAs. In ELISAs, this antibody also recognizes bovine FGF basic but not bovine FGF acidic, recombinant human (rh) FGF acidic, rhFGF‑4, rhFGF‑6, or rhFGF‑7.

Source

Monoclonal Mouse IgG_2A Clone # 10043

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FGF basic/FGF2/bFGF

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Human FGF basic/FGF2/bFGF Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human FGF basic/FGF2/bFGF Antibody (Catalog # MAB233)

Capture Reagent: Human FGF basic/FGF2/bFGF Antibody (Catalog # MAB233R)

Standard: Recombinant Human FGF basic/FGF2/bFGF (146 aa) Protein (Catalog # 233-FB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF basic/FGF2/bFGF

FGF basic, also known as FGF-2 and HBGF-2, is a member of the FGF family of mitogenic proteins. FGF basic is widely expressed and affects diverse aspects of embryonic development by regulating cell proliferation, differentiation, and survival. Various N-terminal extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. Mature human FGF basic shares greater than 96% aa sequence identity with bovine, mouse, and rat FGF basic.

Long Name

Fibroblast Growth Factor basic

Entrez Gene IDs

2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)

Alternate Names

basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin

Product Datasheets

Product Datasheet

Related Research Areas

Citation for Human FGF basic/FGF2/bFGF Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development