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Human FGF basic/FGF2/bFGF Antibody

Catalog #: AF-233-NA
27 Citations Datasheet
Catalog # Availability Size / Price Qty
AF-233-NA
AF-233-SP
FGF basic/FGF2/bFGF in Human Placenta.
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Citations (27)
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Human FGF basic/FGF2/bFGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human and bovine FGF basic/FGF2/bFGF in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant mouse FGF basic/FGF2/bFGF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Bovine brain-derived FGF basic/FGF2/bFGF
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FGF basic/FGF2/bFGF (146 aa) (Catalog # 233-FB)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize FGF basic/FGF2/bFGF-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 0.08-0.4 µg/mL in the presence of 0.5 ng/mL Bovine FGF basic/FGF2/bFGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry FGF basic/FGF2/bFGF antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

FGF basic/FGF2/bFGF in Human Placenta. FGF basic/FGF2/bFGF was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human FGF basic/FGF2/bFGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-233-NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Proliferation Induced by FGF basic/FGF2/bFGF and Neutralization by Human FGF basic/FGF2/bFGF Antibody. View Larger

Cell Proliferation Induced by FGF basic/FGF2/bFGF and Neutralization by Human FGF basic/FGF2/bFGF Antibody. Bovine FGF basic/FGF2/bFGF (Catalog # 133-FB) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF basic/FGF2/bFGF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF basic/FGF2/bFGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-233-NA). The ND50 is typically 0.08-0.4 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF basic/FGF2/bFGF

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35‑60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. A variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron differentiation, survival, and regeneration. FGF basic also modulates embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.

References
  1. Coulier, F. et al. (1997) J. Mol. Evol. 44:43.
  2. Chen, C.H. et al. (2004) Curr. Vasc. Pharmacol. 2:33.
  3. Mohammadi, M. et al. (2005) Curr. Opin. Struct. Biol. 15:506.
  4. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.
Long Name
Fibroblast Growth Factor basic
Entrez Gene IDs
2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin

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Citations for Human FGF basic/FGF2/bFGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. XBP1‐elicited environment by chemotherapy potentiates repopulation of tongue cancer cells by enhancing miR‐22/lncRNA/KAT6B‐dependent NF‐ kappa B signalling
    Authors: Xiaoting Jia, Ge Wang, Lihong Wu, Hao Pan, Li Ling, Jianlei Zhang et al.
    Clinical and Translational Medicine
  2. Fibroblast growth factor-2 bound to specific dermal fibroblast-derived extracellular vesicles is protected from degradation
    Authors: I Petit, A Levy, S Estrach, CC Féral, AG Trentin, F Dingli, D Loew, J Qu, H Zhou, C Théry, C Prunier, D Aberdam, O Ferrigno
    Scientific Reports, 2022-12-22;12(1):22131.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Bioassay, Flow Cytometry, Western Blot
  3. Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion
    Authors: GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva
    Redox Biology, 2021-12-05;48(0):102205.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Dot Blot
  4. Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms.
    Authors: Haichuan H, Zofia P, Patricia H et al.
    Cancer Cell.
  5. A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapy
    Authors: J Li, A Wuethrich, AAI Sina, HH Cheng, Y Wang, A Behren, PN Mainwaring, M Trau
    Nature Communications, 2021-02-17;12(1):1087.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  6. Inhibition of protein glycosylation is a novel pro-angiogenic strategy that acts via activation of stress pathways
    Authors: C Zhong, P Li, S Argade, L Liu, A Chilla', W Liang, H Xin, B Eliceiri, B Choudhury, N Ferrara
    Nature Communications, 2020-12-10;11(1):6330.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Metastatic Brain Tumors Disrupt the Blood-Brain Barrier and Alter Lipid Metabolism by Inhibiting Expression of the Endothelial Cell Fatty Acid Transporter Mfsd2a
    Authors: S Tiwary, JE Morales, SC Kwiatkowsk, FF Lang, G Rao, JH McCarty
    Sci Rep, 2018-05-29;8(1):8267.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Tumor-associated B-cells induce tumor heterogeneity and therapy resistance
    Authors: R Somasundar, G Zhang, M Fukunaga-K, M Perego, C Krepler, X Xu, C Wagner, D Hristova, J Zhang, T Tian, Z Wei, Q Liu, K Garg, J Griss, R Hards, M Maurer, C Hafner, M Mayerhöfer, G Karanikas, A Jalili, V Bauer-Pohl, F Weihsengru, K Rappersber, J Koller, R Lang, C Hudgens, G Chen, M Tetzlaff, L Wu, DT Frederick, RA Scolyer, GV Long, M Damle, C Ellingswor, L Grinman, H Choi, BJ Gavin, M Dunagin, A Raj, N Scholler, L Gross, M Beqiri, K Bennett, I Watson, H Schaider, MA Davies, J Wargo, BJ Czerniecki, L Schuchter, D Herlyn, K Flaherty, M Herlyn, SN Wagner
    Nat Commun, 2017-09-19;8(1):607.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Pericytes impair capillary blood flow and motor function after chronic spinal cord injury
    Authors: Y Li, AM Lucas-Osma, S Black, MV Bandet, MJ Stephens, R Vavrek, L Sanelli, KK Fenrich, AF Di Narzo, S Dracheva, IR Winship, K Fouad, DJ Bennett
    Nat. Med., 2017-05-01;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  11. Discovery of pan-VEGF inhibitory peptides directed to the extracellular ligand-binding domains of the VEGF receptors
    Authors: Jussara S. Michaloski, Alexandre R. Redondo, Leila S. Magalhães, Caio C. Cambui, Ricardo J. Giordano
    Science Advances
  12. Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts.
    Authors: Angelucci C, Maulucci G, Colabianchi A, Iacopino F, D'Alessio A, Maiorana A, Palmieri V, Papi M, De Spirito M, Di Leone A, Masetti R, Sica G
    Br J Cancer, 2015-04-16;112(10):1675-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Peroxynitrite upregulates angiogenic factors VEGF-A, BFGF, and HIF-1alpha in human corneal limbal epithelial cells.
    Authors: Ashki N, Chan A, Qin Y, Wang W, Kiyohara M, Lin L, Braun J, Wadehra M, Gordon L
    Invest Ophthalmol Vis Sci, 2014-03-19;55(3):1637-46.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Endothelial heparan sulfate 6-o-sulfation levels regulate angiogenic responses of endothelial cells to fibroblast growth factor 2 and vascular endothelial growth factor.
    Authors: Ferreras, Cristina, Rushton, Graham, Cole, Claire L, Babur, Muhammad, Telfer, Brian A, van Kuppevelt, Toin H, Gardiner, John M, Williams, Kaye J, Jayson, Gordon C, Avizienyte, Egle
    J Biol Chem, 2012-08-27;287(43):36132-46.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Inhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration via production of angiocrine factors in mice.
    Blood, 2012-05-09;119(26):6382-93.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  16. Bone marrow stroma-secreted cytokines protect JAK2(V617F)-mutated cells from the effects of a JAK2 inhibitor
    Authors: Taghi Manshouri, Zeev Estrov, Alfonso Quintás-Cardama, Jan Burger, Ying Zhang, Ana Livun et al.
    Cancer Research
  17. The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin.
    Authors: Choi W, Wolber R, Gerwat W
    J. Cell. Sci., 2010-08-24;123(0):3102-11.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. IFATS Series: FGF-2-induced HGF Secretion By Adipose-Derived Stromal Cells Inhibits Post-Injury Fibrogenesis Through A JNK-Dependent Mechanism.
    Authors: Suga H, Eto H, Shigeura T, Inoue K, Aoi N, Kato H, Nishimura S, Manabe I, Gonda K, Yoshimura K
    Stem Cells, 2009-01-01;0(0):.
    Species: Mouse
    Sample Types: In Vivo, Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Neutralization, Western Blot
  19. A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells.
    Authors: Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC
    Exp. Cell Res., 2008-04-25;314(11):2176-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Temporally expressed PDGF and FGF-2 regulate embryonic coronary artery formation and growth.
    Authors: Tomanek RJ, Hansen HK, Christensen LP
    Arterioscler. Thromb. Vasc. Biol., 2008-04-17;28(7):1237-43.
    Species: Avian
    Sample Types: Whole Tissue
    Applications: Neutralization
  21. Pancreatic stellate cells: partners in crime with pancreatic cancer cells.
    Authors: Vonlaufen A, Joshi S, Qu C, Phillips PA, Xu Z, Parker NR, Toi CS, Pirola RC, Wilson JS, Goldstein D, Apte MV
    Cancer Res., 2008-04-01;68(7):2085-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Agrin, a novel basement membrane component in human and rat liver, accumulates in cirrhosis and hepatocellular carcinoma.
    Authors: Tatrai P, Dudas J, Batmunkh E, Máthé M, Zalatnai A, Schaff Z, Ramadori G, Kovalszky I
    Lab. Invest., 2006-09-18;86(11):1149-60.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  23. Positive correlation between estradiol and vascular endothelial growth factor but not fibroblast growth factor-2 in normal human breast tissue in vivo.
    Authors: Dabrosin C
    Clin. Cancer Res., 2005-11-15;11(22):8036-41.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  24. FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis.
    Authors: Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP
    Development, 2005-02-16;132(6):1223-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Differential role of platelet granular mediators in angiogenesis.
    Authors: Brill A, Elinav H, Varon D
    Cardiovasc. Res., 2004-08-01;63(2):226-35.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
    Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V
    Blood, 2003-03-20;102(2):638-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. The anti-HIV cytokine midkine binds the cell surface-expressed nucleolin as a low affinity receptor.
    Authors: Said EA, Krust B, Nisole S, Svab J, Briand JP, Hovanessian AG
    J. Biol. Chem., 2002-07-29;277(40):37492-502.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: ICC

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